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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481S-adenosyl-L-methionineUniRule annotation
Binding sitei73 – 731S-adenosyl-L-methionineUniRule annotation
Binding sitei100 – 1001S-adenosyl-L-methionineUniRule annotation
Active sitei123 – 1231By similarity
Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation
Binding sitei127 – 1271SubstrateUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLBOR355277:GHYM-120-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:LBJ_0122
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
ProteomesiUP000000656 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferasePRO_0000288169Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ04W54.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000040678.
KOiK03439.
OMAiYICHLAR.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04W54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDLEQKLW SIASGIPFVS DYFLQASPAR KLKKENLFSK VFETYFLELG
60 70 80 90 100
SGWGEVAIAM ALQRRNTGFV LMEKKFDRIR HTIREIEKHS LDNVKILCVN
110 120 130 140 150
FNWFLADVFE ENLFSEILLN FPDPWPKKRH HKKRTINSKF IESLRVLLPE
160 170 180 190 200
KGKFSFATDY GPYARKTIRL FRDSEIFKPE TMEFRLERKE IPVSHFERKK
210
RKEGKRIYYI DQILIRK
Length:217
Mass (Da):26,000
Last modified:November 14, 2006 - v1
Checksum:iCE903272344ADD04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA. Translation: ABJ74866.1.
RefSeqiWP_011671187.1. NC_008510.1.
YP_799624.1. NC_008510.1.

Genome annotation databases

EnsemblBacteriaiABJ74866; ABJ74866; LBJ_0122.
GeneIDi4406027.
KEGGilbj:LBJ_0122.
PATRICi22354822. VBILepBor13265_0141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA. Translation: ABJ74866.1.
RefSeqiWP_011671187.1. NC_008510.1.
YP_799624.1. NC_008510.1.

3D structure databases

ProteinModelPortaliQ04W54.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ74866; ABJ74866; LBJ_0122.
GeneIDi4406027.
KEGGilbj:LBJ_0122.
PATRICi22354822. VBILepBor13265_0141.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000040678.
KOiK03439.
OMAiYICHLAR.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciLBOR355277:GHYM-120-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JB197.

Entry informationi

Entry nameiTRMB_LEPBJ
AccessioniPrimary (citable) accession number: Q04W54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 14, 2006
Last modified: June 24, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.