Q04VR0 (ISPD_LEPBJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	LBJ_0280

Organism

Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) [Complete proteome] [HAMAP]

Taxonomic identifier

355277 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000191061
Sites
	Site	22	1	Transition state stabilizer By similarity
	Site	29	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	214	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04VR0 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: E8E064786C5D2631	FASTA	235	26,639
	10 20 30 40 50 60 MKSLFLSEKI YVLILAGGTG TRMGSEIPKQ FLEFSNEPIL IHTLKKFQSW KKQKQIVLVS 70 80 90 100 110 120 HPEFISETES ICSPFLENQD CIIEGGETRH GSMLRGLSAL TIQSEDILLI HDAARPFVLL 130 140 150 160 170 180 KELDLLCENI RENGISTLAS RTSETVLEES NGKTSSFLDR EHIWFMKTPQ GIRGDILKEL 190 200 210 220 230 LTLPMDPIPT DLCSWALTAG KKSSIVESHP FNLKITRKED LELAEFYSDL FEKLR

References

[1]

"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential." Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B. Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed: 16973745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JB197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000350 Genomic DNA. Translation: ABJ75010.1.
RefSeq	YP_799768.1. NC_008510.1.
3D structure databases
ProteinModelPortal	Q04VR0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04VR0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4409941.
GenomeReviews	Gene locus LBJ_0280 in contig CP000350_GR.
KEGG	lbj:LBJ_0280.
PATRIC	22355240. VBILepBor13265_0347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	REQQDFW.
PhylomeDB	Q04VR0.
ProtClustDB	CLSK573749.
Enzyme and pathway databases
BioCyc	LBOR355277:LBJ_0280-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_LEPBJ

Accession

Primary (citable) accession number: Q04VR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program