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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55Substrate; via amide nitrogenUniRule annotation1
Binding sitei130ATPUniRule annotation1
Binding sitei176ATPUniRule annotation1
Binding sitei203Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:LBJ_0526
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000000656 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000215161 – 264Hydroxyethylthiazole kinaseAdd BLAST264

Proteomic databases

PRIDEiQ04V49

Structurei

3D structure databases

ProteinModelPortaliQ04V49
SMRiQ04V49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG091H004S

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

Q04V49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKHSALERA WPAKEIVEDL LELRKRSPLT HIMTNIVVTN WTANVLLAVG
60 70 80 90 100
ASPAMVIAEE EAGEFVKIAN GLLINVGTIT SNDAKAMKIA ATVAHQTNTP
110 120 130 140 150
WVLDPVAVGA LGFRTETTKK LLDLKPTVIR GNASEILTLA GIAGKGKGVD
160 170 180 190 200
STVNSKDALP YAQELSGKTG AVVAVSGEVD YVTNGKETIE IYGGDPIMTK
210 220 230 240 250
VTGVGCSLGA LIASFLGIQK DPLRASASAS AVFAIAGSRS AKKSNGPGSF
260
AINFIDQLSQ LSIE
Length:264
Mass (Da):27,495
Last modified:November 14, 2006 - v1
Checksum:iFB4EEF5732113255
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA Translation: ABJ75221.1
RefSeqiWP_002738770.1, NC_008510.1

Genome annotation databases

EnsemblBacteriaiABJ75221; ABJ75221; LBJ_0526
GeneIDi4407884
KEGGilbj:LBJ_0526

Similar proteinsi

Entry informationi

Entry nameiTHIM_LEPBJ
AccessioniPrimary (citable) accession number: Q04V49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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