Q04V49 (THIM_LEPBJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	LBJ_0526

Organism

Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) [Complete proteome] [HAMAP]

Taxonomic identifier

355277 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021516
Sites
	Binding site	55	1	Substrate; via amide nitrogen By similarity
	Binding site	130	1	ATP By similarity
	Binding site	176	1	ATP By similarity
	Binding site	203	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04V49 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: FB4EEF5732113255	FASTA	264	27,495
	10 20 30 40 50 60 MPKHSALERA WPAKEIVEDL LELRKRSPLT HIMTNIVVTN WTANVLLAVG ASPAMVIAEE 70 80 90 100 110 120 EAGEFVKIAN GLLINVGTIT SNDAKAMKIA ATVAHQTNTP WVLDPVAVGA LGFRTETTKK 130 140 150 160 170 180 LLDLKPTVIR GNASEILTLA GIAGKGKGVD STVNSKDALP YAQELSGKTG AVVAVSGEVD 190 200 210 220 230 240 YVTNGKETIE IYGGDPIMTK VTGVGCSLGA LIASFLGIQK DPLRASASAS AVFAIAGSRS 250 260 AKKSNGPGSF AINFIDQLSQ LSIE

References

[1]

"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential." Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B. Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed: 16973745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JB197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000350 Genomic DNA. Translation: ABJ75221.1.
RefSeq	YP_799979.1. NC_008510.1.
3D structure databases
ProteinModelPortal	Q04V49.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04V49.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4409425.
GenomeReviews	Gene locus LBJ_0526 in contig CP000350_GR.
KEGG	lbj:LBJ_0526.
PATRIC	22355905. VBILepBor13265_0675.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HBG351126.
OMA	AIRGNAG.
PhylomeDB	Q04V49.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	LBOR355277:LBJ_0526-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. ThiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_LEPBJ

Accession

Primary (citable) accession number: Q04V49

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program