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Q04T63

- LIPA_LEPBJ

UniProt

Q04T63 - LIPA_LEPBJ

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Protein

Lipoyl synthase

Gene
lipA, LBJ_1322
Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi80 – 801Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi84 – 841Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLBOR355277:GHYM-1307-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:LBJ_1322
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000000656: Chromosome 1

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Lipoyl synthaseUniRule annotationPRO_0000325271Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi355277.LBJ_1322.

Structurei

3D structure databases

ProteinModelPortaliQ04T63.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPNIAECF.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04T63-1 [UniParc]FASTAAdd to Basket

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MINPLKKKPR THFLQKAPEK PDWLKVKLTF PDPKNNPVAI VRNSLEKKKL    50
NTVCESASCP NLNHCWSRKT ATYMLGGDIC TRRCSYCDVA SGKPSALDRD 100
EPKRVAESAI ALGLKHVVIT AVNRDDLEDG GAAHFAETVE VVREGLPDCK 150
IELLVPDFKV RPESLEIIFQ CKPDIFNHNV ETIKRLFPEV APQKKYERSL 200
DVLKIASEKG FLTKSGLILG MGETVEEVKE CMRDLIGVGV SLLTLGQYLQ 250
PTPTHLPVKS YVLPEVFQEL RIYGKSIGFK GVFSGPLVRS SYHADEQVSW 300
NP 302
Length:302
Mass (Da):33,783
Last modified:November 14, 2006 - v1
Checksum:i578BA357D48634CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000350 Genomic DNA. Translation: ABJ75907.1.
RefSeqiYP_800665.1. NC_008510.1.

Genome annotation databases

EnsemblBacteriaiABJ75907; ABJ75907; LBJ_1322.
GeneIDi4410289.
KEGGilbj:LBJ_1322.
PATRICi22357968. VBILepBor13265_1697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000350 Genomic DNA. Translation: ABJ75907.1 .
RefSeqi YP_800665.1. NC_008510.1.

3D structure databases

ProteinModelPortali Q04T63.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 355277.LBJ_1322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ75907 ; ABJ75907 ; LBJ_1322 .
GeneIDi 4410289.
KEGGi lbj:LBJ_1322.
PATRICi 22357968. VBILepBor13265_1697.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PNIAECF.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci LBOR355277:GHYM-1307-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JB197.

Entry informationi

Entry nameiLIPA_LEPBJ
AccessioniPrimary (citable) accession number: Q04T63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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