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Q04RS9

- BIOB_LEPBJ

UniProt

Q04RS9 - BIOB_LEPBJ

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Protein
Biotin synthase
Gene
bioB, LBJ_1870
Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi146 – 1461Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi206 – 2061Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi276 – 2761Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLBOR355277:GHYM-1846-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:LBJ_1870
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000000656: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Biotin synthaseUniRule annotation
PRO_0000381443Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi355277.LBJ_1870.

Structurei

3D structure databases

ProteinModelPortaliQ04RS9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiFEIANEC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04RS9-1 [UniParc]FASTAAdd to Basket

« Hide

MSEKMYATLK TAEKIFSEIS SVITKQEGLE ILNGSIPLTT CLDKAFQERN    50
RYFYNKVRIH ILDNIKNGYC PEDCGYCAQR KNANSGIKEY PMKSEAEIYE 100
DAVQAKKNGA YRFCMVTSGT GPNRLTTERL ASTIRRITNE LGMKVCLSAG 150
LLDEEKAQVL KSAGLDRYNH NLNTSENHYP EICDTHTYAQ RTQTLDSVSK 200
AGIGMCSGVI VGMGESFQDI VDMAFELKSF RVISIPVNFF IPVKGHAIKN 250
PGILTPELCV RILCLFRLVN PDSEIRIAAG REGHLRSLSA MALFAANSLF 300
SSGYLNVKGS EIIETVTMIR DAGFVPELVD GGILPEESGT EMLYSEKNFP 350
ELYKFKKS 358
Length:358
Mass (Da):39,844
Last modified:November 14, 2006 - v1
Checksum:i77B9930EF3CF4746
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000350 Genomic DNA. Translation: ABJ76391.1.
RefSeqiYP_801149.1. NC_008510.1.

Genome annotation databases

EnsemblBacteriaiABJ76391; ABJ76391; LBJ_1870.
GeneIDi4410110.
KEGGilbj:LBJ_1870.
PATRICi22359390. VBILepBor13265_2402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000350 Genomic DNA. Translation: ABJ76391.1 .
RefSeqi YP_801149.1. NC_008510.1.

3D structure databases

ProteinModelPortali Q04RS9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 355277.LBJ_1870.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ76391 ; ABJ76391 ; LBJ_1870 .
GeneIDi 4410110.
KEGGi lbj:LBJ_1870.
PATRICi 22359390. VBILepBor13265_2402.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi FEIANEC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci LBOR355277:GHYM-1846-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JB197.

Entry informationi

Entry nameiBIOB_LEPBJ
AccessioniPrimary (citable) accession number: Q04RS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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