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Q04QS6

- SYI_LEPBJ

UniProt

Q04QS6 - SYI_LEPBJ

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LBJ_2270
Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei557 – 5571Aminoacyl-adenylate By similarity
Binding sitei601 – 6011ATP By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi892 – 8921Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLBOR355277:GHYM-2241-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LBJ_2270
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000000656: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022088Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi355277.LBJ_2270.

Structurei

3D structure databases

ProteinModelPortaliQ04QS6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionUniRule annotation
Add
BLAST
Motifi598 – 6025"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04QS6-1 [UniParc]FASTAAdd to Basket

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MSETQKENPY SSTVLLPKTD FPMKADLAKR EPEQIRSWKQ NRIFRKMREQ    50
RSGKKEFVLH DGPPYANGNF HLGHALNKIL KDTIIKSKSL AGFYADMIPG 100
WDCHGLPIEV QVLKNLGKKV RETGPEELRQ LCRKYAEEFV GKQGDDLSRF 150
LCFWDEGRIY KTMSPDFEAK IVEVFGELFK KGYVYRGKKP VYWSIDLATA 200
HAEAEIEYYP HISPSIYVKF PIIGEKKRFC LIWTTTPWTL PANLAICFNR 250
KIEYSIFKTE SSEELILADA LAENVTITTG VALTKLKPIT SEELAALKFQ 300
HPFVDRISVS LFGDHVTLEA GTGCVHTAPG HGQDDYKVGL AAGLEPFSPV 350
DDYGRYTDEF PLMQGKKVFD ANPEIIQLLR DKGLLLYHGE LEHSYPHSWR 400
SKKPLIFRAT PQWFFKIDFQ DLREKSLSAI DGVRWIPSWG ITRIRSMVET 450
RPDWCLSRQR NWGVPIPAFT CESCGQTHID DASIQFFTKM VREKGIEIWY 500
SEETKDLLPP KTKCGKCGND SFKKGNDILD VWFDSGVSNF SVLGERKDEP 550
PADLYLEGSD QHRGWFQSSL WPSMALRGIP PYKAVLTHGY VLDEKGRAMS 600
KSLGNGIDPT ADIIQVYGAD ILRLWVSSLD FRDDIKVGKE SLKIVSEQYR 650
KIRNTFRYLL GNLDGHTPEQ NLPFEELEEL DRFYLSKLAG FVEDAVASYE 700
TYQFHQIYQK LILFCTVTLS QDYFDMIRDR MYCDLRDSKS RRSSSTALQY 750
ILDSLCILVA PILSFTAEEV WTSNGKKDSV FLQTFPDLKS WKNQSLEDKF 800
ESALQAREVV QKALEIARQE GKLGKSLEAA LEIVSKSGLS FGELLPKETL 850
ELLFVVSQIH EENPGMEVLS SHENEKFSVK VLKPLQGECP RCWRHTEDIS 900
KEGDLCGRCK SVVA 914
Length:914
Mass (Da):104,458
Last modified:November 14, 2006 - v1
Checksum:i32F8806EE48D446C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000350 Genomic DNA. Translation: ABJ76744.1.
RefSeqiYP_801502.1. NC_008510.1.

Genome annotation databases

EnsemblBacteriaiABJ76744; ABJ76744; LBJ_2270.
GeneIDi4410993.
KEGGilbj:LBJ_2270.
PATRICi22360424. VBILepBor13265_2915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000350 Genomic DNA. Translation: ABJ76744.1 .
RefSeqi YP_801502.1. NC_008510.1.

3D structure databases

ProteinModelPortali Q04QS6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 355277.LBJ_2270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ76744 ; ABJ76744 ; LBJ_2270 .
GeneIDi 4410993.
KEGGi lbj:LBJ_2270.
PATRICi 22360424. VBILepBor13265_2915.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LBOR355277:GHYM-2241-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JB197.

Entry informationi

Entry nameiSYI_LEPBJ
AccessioniPrimary (citable) accession number: Q04QS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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