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Protein

UvrABC system protein B

Gene

uvrB

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:LBJ_2353
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000000656 Componenti: Chromosome 1

Subcellular locationi

Q04QJ9:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000779021 – 666UvrABC system protein BAdd BLAST666

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ04QJ9.
SMRiQ04QJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 183Helicase ATP-bindingUniRule annotationAdd BLAST158
Domaini429 – 591Helicase C-terminalUniRule annotationAdd BLAST163
Domaini625 – 660UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 115Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04QJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIFKIHSA YKPAGDQIKA IENIADSFQK GAEKVTLVGV TGSGKTFTMA
60 70 80 90 100
QVIQNLGLPT LVLSHNKTLA AQLFREFKEF FPENAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPSSDTFIE KDSSINEEID KLRLRATSSL LEREDVVIVS SVSCIYGLGS
160 170 180 190 200
PEEYTNSVVA LKTGDTIERD AVIRKLLHIQ YNRNDIDFSR GNFRVRGDSI
210 220 230 240 250
EIYPAYHTDG IRIEFFGDEI DSISRINPIT AQTILKLEKT YIYPAKHFIT
260 270 280 290 300
SGPKVKVAIE NIKAELEAQA LFFRKNDKLL EAERIISRTN YDMEMLQEMG
310 320 330 340 350
YCNGIENYSR HLTGRKAGER PACLIDYFQG EFLLIVDESH VTIPQIGGMF
360 370 380 390 400
AGDKARKQTL VDFGFRLPSA LDNRPLNFEE FETLTPKTLY VSATPADYEM
410 420 430 440 450
EKSSKVVEQI IRPTGLLDPI VEVRSTKNQI EDLLVEIRKR IDVGERILIT
460 470 480 490 500
TLTKKMSEDL TDYYKEIGLQ VAYLHSEVET LDRVAIIRDL RKGIYDVLIG
510 520 530 540 550
INLLREGLDI PEVSLVAILD ADKEGFLRNY KSLIQTVGRA ARNVNGTAIL
560 570 580 590 600
YADKITDSMA KAIDETKRRR KIQEDHNLKF GITPLTIRKE VNDIIEREEK
610 620 630 640 650
KRTSEDLVLE DVEKKFNSKK FPNKEVLKDK LREEMMKAAK ELDFERAAIL
660
RDKMLSIQIN DPSTEN
Length:666
Mass (Da):75,950
Last modified:November 14, 2006 - v1
Checksum:i21067FF2E38BBC79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA. Translation: ABJ76821.1.
RefSeqiWP_011669628.1. NC_008510.1.

Genome annotation databases

EnsemblBacteriaiABJ76821; ABJ76821; LBJ_2353.
GeneIDi4408448.
KEGGilbj:LBJ_2353.

Similar proteinsi

Entry informationi

Entry nameiUVRB_LEPBJ
AccessioniPrimary (citable) accession number: Q04QJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 14, 2006
Last modified: October 25, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families