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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LBJ_2815
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000000656 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019181 – 521Glutamate--tRNA ligaseAdd BLAST521

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ04PF0
SMRiQ04PF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 23"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q04PF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQNREVRTR FAPSPSGFLH VGGARTALFN YLYAKSQGGK FILRIEDTDQ
60 70 80 90 100
NRSTEDSFKI ILESLKWLGV RWDEGPEIGG EYGPYIQSQR LDIYKEYTEK
110 120 130 140 150
LLKEKKAYRC FCTQEELEAK KKQAEAMGVP YVYDGLHANM SDEEVEEKLK
160 170 180 190 200
QKIPYSIRFK TPSKTLIVDD IIQGKVKFET KLIGDFIIVK SDGFPSYNYA
210 220 230 240 250
VVVDDALMKI THVIRGVGHL SNTPRQILLY EALDYEIPEF AHASEIVGMD
260 270 280 290 300
GKKLSKRAGA TSILAFRDLG YLPETFRNYM ALLGWTSSDG REFLPEGELE
310 320 330 340 350
KIFDVHRCSK SPSTFDVFKK PKGGEDEVVT NFSDLTQIAE AMNPKSKLNW
360 370 380 390 400
LSNRTIRDLK ISKVLESLLP FLKDRKDIPE EVKNINSPAL TSIIESIRVY
410 420 430 440 450
LDNLAQAPDY IAEFFVTDLK IQSQEATGFM KDGDGPKVVK EFYGILKNSD
460 470 480 490 500
PKTDENYKDL MSKVGEVTGQ KGKTLYMPIR VATTGKSVGL ELPILFPLLG
510 520
KEKLLQRIEK TAKEAGIPLS T
Length:521
Mass (Da):58,931
Last modified:November 14, 2006 - v1
Checksum:iE19F802C89CAA148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA Translation: ABJ77220.1
RefSeqiWP_011669313.1, NC_008510.1

Genome annotation databases

EnsemblBacteriaiABJ77220; ABJ77220; LBJ_2815
GeneIDi4407455
KEGGilbj:LBJ_2815

Similar proteinsi

Entry informationi

Entry nameiSYE_LEPBJ
AccessioniPrimary (citable) accession number: Q04PF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health