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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei256 – 2561ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLBOR355277:GHYM-2780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LBJ_2815
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
Taxonomic identifieri355277 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
ProteomesiUP000000656 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Glutamate--tRNA ligasePRO_1000001918Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ04PF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 2311"HIGH" regionAdd
BLAST
Motifi253 – 2575"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiEDHITNT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04PF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQNREVRTR FAPSPSGFLH VGGARTALFN YLYAKSQGGK FILRIEDTDQ
60 70 80 90 100
NRSTEDSFKI ILESLKWLGV RWDEGPEIGG EYGPYIQSQR LDIYKEYTEK
110 120 130 140 150
LLKEKKAYRC FCTQEELEAK KKQAEAMGVP YVYDGLHANM SDEEVEEKLK
160 170 180 190 200
QKIPYSIRFK TPSKTLIVDD IIQGKVKFET KLIGDFIIVK SDGFPSYNYA
210 220 230 240 250
VVVDDALMKI THVIRGVGHL SNTPRQILLY EALDYEIPEF AHASEIVGMD
260 270 280 290 300
GKKLSKRAGA TSILAFRDLG YLPETFRNYM ALLGWTSSDG REFLPEGELE
310 320 330 340 350
KIFDVHRCSK SPSTFDVFKK PKGGEDEVVT NFSDLTQIAE AMNPKSKLNW
360 370 380 390 400
LSNRTIRDLK ISKVLESLLP FLKDRKDIPE EVKNINSPAL TSIIESIRVY
410 420 430 440 450
LDNLAQAPDY IAEFFVTDLK IQSQEATGFM KDGDGPKVVK EFYGILKNSD
460 470 480 490 500
PKTDENYKDL MSKVGEVTGQ KGKTLYMPIR VATTGKSVGL ELPILFPLLG
510 520
KEKLLQRIEK TAKEAGIPLS T
Length:521
Mass (Da):58,931
Last modified:November 14, 2006 - v1
Checksum:iE19F802C89CAA148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA. Translation: ABJ77220.1.
RefSeqiWP_011669313.1. NC_008510.1.

Genome annotation databases

EnsemblBacteriaiABJ77220; ABJ77220; LBJ_2815.
GeneIDi4407455.
KEGGilbj:LBJ_2815.
PATRICi22361852. VBILepBor13265_3612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000350 Genomic DNA. Translation: ABJ77220.1.
RefSeqiWP_011669313.1. NC_008510.1.

3D structure databases

ProteinModelPortaliQ04PF0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ77220; ABJ77220; LBJ_2815.
GeneIDi4407455.
KEGGilbj:LBJ_2815.
PATRICi22361852. VBILepBor13265_3612.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiEDHITNT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciLBOR355277:GHYM-2780-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JB197.

Entry informationi

Entry nameiSYE_LEPBJ
AccessioniPrimary (citable) accession number: Q04PF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: July 22, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.