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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationSAAS annotationNote: Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation
  • Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281ZincUniRule annotation
Metal bindingi211 – 2111ZincUniRule annotation
Metal bindingi236 – 2361ZincUniRule annotation
Metal bindingi240 – 2401ZincUniRule annotation
Binding sitei271 – 2711ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciSPNE373153:GIX6-515-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.16UniRule annotationSAAS annotation)
Alternative name(s):
Cysteinyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:cysSUniRule annotationImported
Ordered Locus Names:SPD_0515Imported
OrganismiStreptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)Imported
Taxonomic identifieri373153 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001452 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi373153.SPD_0515.

Structurei

3D structure databases

ProteinModelPortaliQ04LT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi30 – 4011"HIGH" regionUniRule annotationAdd
BLAST
Motifi268 – 2725"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiSMYVCGP.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR001943. UVR_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
PROSITEiPS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04LT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIYDTMSR DLREFVPIED GKIKMYVCGP TVYNYIHVGN ARSTVAFDTI
60 70 80 90 100
RRYFEYRGYK VAYISNFTDV DDKIINRARE EGITPQEVAD KYIAAFREDV
110 120 130 140 150
TALGVKPATR HPRVVEFMAD IIRFVEDLIE RGFAYESQGD VYFRVEKSHN
160 170 180 190 200
YAKLANKTLE DLELGASGRT DEETARKENP VDFALWKSSK PGEISWDSPW
210 220 230 240 250
GPGRPGWHIE CSVMSTEILG DTIDIHGGGA DLEFPHHTNE IAQSEAKTGK
260 270 280 290 300
AFANYWMHNG FVNIDNVKMS KSLGNFITVH DALKTLDGQV LRFFFATQHY
310 320 330 340 350
RKPINFTEKA VRDAETNLKY LKNTYEQPFT GNVDAQELQN FKDKFVAAMD
360 370 380 390 400
EDFNAANGIT VVFEMAKWIN SGNYDASVKQ ALADMLEIFG IVFVEEVLDA
410 420 430 440
EIEDLIQKRQ EARANRDFET ADQIRDQLVT QGIKLLDTKD GVRWTRD
Length:447
Mass (Da):51,081
Last modified:November 14, 2006 - v1
Checksum:iD8D0ED3F63D7958E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000410 Genomic DNA. Translation: ABJ55485.1.
RefSeqiYP_816017.1. NC_008533.1.

Genome annotation databases

EnsemblBacteriaiABJ55485; ABJ55485; SPD_0515.
GeneIDi4441826.
KEGGispd:SPD_0515.
PATRICi19682078. VBIStrPne27904_0579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000410 Genomic DNA. Translation: ABJ55485.1.
RefSeqiYP_816017.1. NC_008533.1.

3D structure databases

ProteinModelPortaliQ04LT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi373153.SPD_0515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ55485; ABJ55485; SPD_0515.
GeneIDi4441826.
KEGGispd:SPD_0515.
PATRICi19682078. VBIStrPne27904_0579.

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiSMYVCGP.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciSPNE373153:GIX6-515-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR001943. UVR_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
PROSITEiPS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6."
    Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., Winkler M.E.
    J. Bacteriol. 189:38-51(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D39 / NCTC 7466Imported.

Entry informationi

Entry nameiQ04LT1_STRP2
AccessioniPrimary (citable) accession number: Q04LT1
Entry historyi
Integrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: April 29, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.