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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 9615-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei100 – 10015-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei102 – 10215-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei126 – 1261OrotateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSPNE373153:GIX6-609-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:SPD_0609
OrganismiStreptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)
Taxonomic identifieri373153 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001452 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Orotate phosphoribosyltransferasePRO_1000066306Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi373153.SPD_0609.

Structurei

3D structure databases

ProteinModelPortaliQ04LJ2.
SMRiQ04LJ2. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 13095-phosphoribose 1-diphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.
OrthoDBiEOG6C2WDW.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04LJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLAKDIASH LLKIQAVYLK PEEPFTWASG IKSPIYTDNR VTLAYPETRT
60 70 80 90 100
LIENGFVEAI KEAFPEVEVI AGTATAGIPH GAIIADKMDL PFAYIRSKPK
110 120 130 140 150
DHGAGNQIEG RVAQGQKMVV VEDLISTGGS VLEAVAAAKR EGADVLGVVA
160 170 180 190 200
IFSYQLPKAD KNFADAGVKL VTLSNYSDLI HLAQEEGYIT PEGLYLLKRF
210
KEDQENWQEG
Length:210
Mass (Da):22,875
Last modified:November 14, 2006 - v1
Checksum:iCA6FA07066AE4163
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000410 Genomic DNA. Translation: ABJ53830.1.
RefSeqiWP_000170913.1. NC_008533.1.

Genome annotation databases

EnsemblBacteriaiABJ53830; ABJ53830; SPD_0609.
KEGGispd:SPD_0609.
PATRICi19682300. VBIStrPne27904_0690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000410 Genomic DNA. Translation: ABJ53830.1.
RefSeqiWP_000170913.1. NC_008533.1.

3D structure databases

ProteinModelPortaliQ04LJ2.
SMRiQ04LJ2. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi373153.SPD_0609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ53830; ABJ53830; SPD_0609.
KEGGispd:SPD_0609.
PATRICi19682300. VBIStrPne27904_0690.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.
OrthoDBiEOG6C2WDW.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciSPNE373153:GIX6-609-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6."
    Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., Winkler M.E.
    J. Bacteriol. 189:38-51(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D39 / NCTC 7466.

Entry informationi

Entry nameiPYRE_STRP2
AccessioniPrimary (citable) accession number: Q04LJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 14, 2006
Last modified: November 11, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.