Q04KZ2 (PROA_STRP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	SPD_0823

Organism

Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) [Complete proteome] [HAMAP]

Taxonomic identifier

373153 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049995

Sequences

	Sequence		Length	Mass (Da)
	Q04KZ2 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: C502599D319F7D94	FASTA	420	45,225
	10 20 30 40 50 60 MVSRQEQFEQ VQAVKKSINT ASEEVKNQAL LAMADHLVAA TEEILAANAL DMAAAKGKIS 70 80 90 100 110 120 DVMLDRLYLD ADRIEAMARG IREVVALPDP IGEVLETSQL ENGLVITKKR VAMGVIGIIY 130 140 150 160 170 180 ESRPNVTSDA AALTLKSGNA VVLRSGKDAY QTTHAIVTAL KKGLETTTIH PNVIQLVEDT 190 200 210 220 230 240 SRESSYAMMK AKGYLDLLIP RGGAGLINAV VENAIVPVIE TGTGIVHVYV DKDADEDKAL 250 260 270 280 290 300 SIINNAKTSR PSVCNAMEVL LVHENKAASI LPRLDQMLVA ERKEAGLEPI QFRLDSKASQ 310 320 330 340 350 360 FVSGQAAETQ DFDTEFLDYV LAVKVVSSLE EAVAHIESHS THHSDAIVTE NAEAAAYFTD 370 380 390 400 410 420 QVDSAAVYVN ASTRFTDGGQ FGLGCEMGIS TQKLHARGPM GLKELTSYKY VVAGDGQIRE

References

[1]

"Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6." Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., Winkler M.E. J. Bacteriol. 189:38-51(2007) [PubMed: 17041037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D39 / NCTC 7466.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000410 Genomic DNA. Translation: ABJ54450.1.
RefSeq	YP_816306.1. NC_008533.1.
3D structure databases
ProteinModelPortal	Q04KZ2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04KZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000018820; EBSTRP00000018060; EBSTRG00000018820.
GeneID	4442516.
GenomeReviews	Gene locus SPD_0823 in contig CP000410_GR.
KEGG	spd:SPD_0823.
PATRIC	19682782. VBIStrPne27904_0928.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
GeneTree	EBGT00050000027638.
HOGENOM	HBG318080.
OMA	HRIEAMA.
ProtClustDB	PRK00197.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_STRP2

Accession

Primary (citable) accession number: Q04KZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program