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Q04KG8 (GLGB_STRP2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:SPD_1005
OrganismStreptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) [Complete proteome] [HAMAP]
Taxonomic identifier373153 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length642 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6426421,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000045002

Sites

Active site3041Nucleophile By similarity
Active site3551Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04KG8 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 1806CF2A59D7AEC0

FASTA64275,542
        10         20         30         40         50         60 
MDNREALKTF MTGENFYLQH YLGAHREELN GEHGYTFRVW APNAQAVHLV GDFTNWIENQ 

        70         80         90        100        110        120 
IPMVRNDFGV WEVFTNMAQE GHIYKYHVTR QNGHQLMKID PFAVRYEARP GTGAIVTELP 

       130        140        150        160        170        180 
EKKWKDGLWL ARRKRWGFAE RPVNIYEVHA GSWKRNPDGS PYSFAQLKDE LIPYLVEMNY 

       190        200        210        220        230        240 
THIEFMPLMS HPLGLSWGYQ LMGYFALEHA YGRPEEFQDF VEECHTHNIG VIVDWVPGHF 

       250        260        270        280        290        300 
TINDDALAYY DGTPTFEYQD HNKAHNHGWG ALNFDLGKNE VQSFLISCIK HWIDVYHLDG 

       310        320        330        340        350        360 
IRVDAVSNML YLDYDDAPWT PNKDGGNLNY EGYYFLQRLN EVIKLEYPDV MMIAEESSSA 

       370        380        390        400        410        420 
TKITGMKEIG GLGFDYKWNM GWMNDILRFY EEDPIYRKYD FNLVTFSFMY VFKENYLLPF 

       430        440        450        460        470        480 
SHDEVVHGKK SMMHKMWGDR YNQFAGLRNL YTYQICHPGK KLLFMGSEYG QFLEWKSEEQ 

       490        500        510        520        530        540 
LEWSNLEDPM NAKMKYFTSQ LNQFYKDHRC LWEIDTSYDG IEIIDADNRD QSVLSFIRKG 

       550        560        570        580        590        600 
KKGEMLVCIF NMVPVERKDF TIGLPVAGIY EEVWNTELEE WGGVWKEHNQ TVQTQEGLWK 

       610        620        630        640 
DYEQTLTFTL PAMGASVWKI KRRLKSTKTV TNKNPKGVEN EK 

« Hide

References

[1]"Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6."
Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., Winkler M.E.
J. Bacteriol. 189:38-51(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: D39 / NCTC 7466.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000410 Genomic DNA. Translation: ABJ55433.1.
RefSeqYP_816480.1. NC_008533.1.

3D structure databases

ProteinModelPortalQ04KG8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING373153.SPD_1005.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ55433; ABJ55433; SPD_1005.
GeneID4442375.
KEGGspd:SPD_1005.
PATRIC19683176. VBIStrPne27904_1123.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSPNE373153:GIX6-1005-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_STRP2
AccessionPrimary (citable) accession number: Q04KG8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries