Q04K52 (ISPD_STRP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	SPD_1127

Organism

Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) [Complete proteome] [HAMAP]

Taxonomic identifier

373153 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000022951
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	161	1	Positions MEP for the nucleophilic attack By similarity
	Site	218	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04K52 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 0CD17F5952786D97	FASTA	235	26,242
	10 20 30 40 50 60 MIYAGILAGG TGTRMGISNL PKQFLELGDR PILIHTIEKF VLEPSIEKIV VGVHGDWVSH 70 80 90 100 110 120 AEDLVDKYLP LYKERIIITK GGADRNTSIK NIIEAIDAYR PLTPEDIVVT HDSVRPFITL 130 140 150 160 170 180 RMIQDNIQLA QNHDAVDTVV EAVDTIVEST NGQFITDIPN RAHLYQGQTP QTFRCKDFMD 190 200 210 220 230 LYGSLSDEEK EILTDACKIF VIKGKDVALA KGEYSNLKIT TVTDLKIAKS MIEKD

References

[1]

"Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6." Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., Winkler M.E. J. Bacteriol. 189:38-51(2007) [PubMed: 17041037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D39 / NCTC 7466.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000410 Genomic DNA. Translation: ABJ55399.1.
RefSeq	YP_816596.1. NC_008533.1.
3D structure databases
ProteinModelPortal	Q04K52.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04K52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000019995; EBSTRP00000019235; EBSTRG00000019995.
GeneID	4441914.
GenomeReviews	Gene locus SPD_1127 in contig CP000410_GR.
KEGG	spd:SPD_1127.
PATRIC	19683438. VBIStrPne27904_1253.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
GeneTree	EBGT00050000027822.
HOGENOM	HBG672839.
OMA	GELFNIK.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_STRP2

Accession

Primary (citable) accession number: Q04K52

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program