Q04IJ8 (ASNA_STRP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	SPD_1768

Organism

Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) [Complete proteome] [HAMAP]

Taxonomic identifier

373153 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017964

Sequences

	Sequence		Length	Mass (Da)
	Q04IJ8 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: BF01477215EC39C5	FASTA	330	37,608
	10 20 30 40 50 60 MKKSFIHQQE EISFVKNTFT QYLKDKLEVV EVQGPILSKV GDGMQDNLSG VENPVSVKVL 70 80 90 100 110 120 QIPDATYEVV HSLAKWKRHT LARFGFGEGE GLFVHMKALR PDEDSLDATH SVYVDQWDWE 130 140 150 160 170 180 KVIPNGKRNI VYLKETVEKI YKAIRLTELA VEARYDIESI LPKQITFIHT EELVERYPDL 190 200 210 220 230 240 TSKERENAIC KEFGAVFLIG IGGELPDGKP HDGRAPDYDD WTSESENGYK GLNGDILVWN 250 260 270 280 290 300 ESLGGAFELS SMGIRVDEET LRRQVEITGD EDRLELEWHK SLLNGLFPLT IGGGIGQSRM 310 320 330 AMFLLRKRHI GEVQTSVWPQ EVRDTYENIL

References

[1]

"Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6." Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., Wayne K.J., Tettelin H., Glass J.I., Winkler M.E. J. Bacteriol. 189:38-51(2007) [PubMed: 17041037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D39 / NCTC 7466.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000410 Genomic DNA. Translation: ABJ54080.1.
RefSeq	YP_817190.1. NC_008533.1.
3D structure databases
ProteinModelPortal	Q04IJ8.
SMR	Q04IJ8. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04IJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000020409; EBSTRP00000019649; EBSTRG00000020409.
GeneID	4441684.
GenomeReviews	Gene locus SPD_1768 in contig CP000410_GR.
KEGG	spd:SPD_1768.
PATRIC	19684846. VBIStrPne27904_1933.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
GeneTree	EBGT00050000027962.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_STRP2

Accession

Primary (citable) accession number: Q04IJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program