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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Oenococcus oeni (strain ATCC BAA-331 / PSU-1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei182SubstrateUniRule annotation1
Active sitei183Proton donor/acceptorUniRule annotation1
Active sitei313UniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Sitei326Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:OEOE_0029Imported
OrganismiOenococcus oeni (strain ATCC BAA-331 / PSU-1)Imported
Taxonomic identifieri203123 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus
Proteomesi
  • UP000000774 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi203123.OEOE_0029.

Structurei

3D structure databases

ProteinModelPortaliQ04HN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 337Lyase_1InterPro annotationAdd BLAST324
Domaini404 – 455FumaraseC_CInterPro annotationAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 101Substrate bindingUniRule annotation3
Regioni135 – 137Substrate bindingUniRule annotation3
Regioni319 – 321Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04HN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNEKYRIEK DTLGEVKIPA SALWGPQTER SRQNFKIGQR MPFEIIESLL
60 70 80 90 100
QIKKAAAVVN EKNGEISKEK SNLIVEVIDQ LLVGKYQDDF PLVVYQTGSG
110 120 130 140 150
TQTNMNVNEV IVHLAAEINP KVLLQANDDV NHSQSSNDTF PTAMAIAAYD
160 170 180 190 200
AVKKLLSVLE HLENSLNDKA LEFKNIVKIG RTHLQDATPI TFGQEISGWS
210 220 230 240 250
SQIKHNFDYL QNNKKTLLEL PIGGTAVGTG LNTVKDFDRQ MVEQLSVQKG
260 270 280 290 300
ISYLVAPNKF QGLAAHSAIN FIHGIFKTLA ADLVKIGNDI RFLASGPRSG
310 320 330 340 350
YGEINIPANE PGSSIMPGKI NPTQIEALTM VAARVMGNDT TIDFSSSQGN
360 370 380 390 400
FEMNVYKPII IQSFLESAKL LAEAIGSVDK NLIQGITVNQ KRMKDLVDNS
410 420 430 440 450
LMTVTALSPH IGYEKSAKIA QKALRENTKL IDAALASGYV TEKEFREWVD

PFKMTSHK
Length:458
Mass (Da):50,516
Last modified:November 14, 2006 - v1
Checksum:iBEE4D1FA62AA89A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ56033.1.
RefSeqiWP_011677334.1. NC_008528.1.

Genome annotation databases

EnsemblBacteriaiABJ56033; ABJ56033; OEOE_0029.
GeneIDi4416180.
KEGGiooe:OEOE_0029.
PATRICifig|203123.7.peg.29.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiQ04HN9_OENOB
AccessioniPrimary (citable) accession number: Q04HN9
Entry historyiIntegrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: June 7, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported