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Q04GX5 (SYE_OENOB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:OEOE_0321
OrganismOenococcus oeni (strain ATCC BAA-331 / PSU-1) [Complete proteome] [HAMAP]
Taxonomic identifier203123 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesOenococcus

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_1000057197

Regions

Motif11 – 2111"HIGH" region HAMAP-Rule MF_00022
Motif261 – 2655"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2641ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04GX5 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 7B16BE9CFBC634B4

FASTA49857,050
        10         20         30         40         50         60 
MTEKIRVRYA PSPTGHLHIG NARTAIFNWL FARHYNGQFI IRIEDTDLAR NVKDGEKSQL 

        70         80         90        100        110        120 
DNLQWLGIDW DEGPDKANSK YAPYRQTERA AQGVYQKYID ELLASGKAYK SYKTEATLKE 

       130        140        150        160        170        180 
EREAQAQAHQ APHYVYEYAG MTKEQIKDAQ AKDEAAGLKS VVRFRVPENH DYQWQDLVKG 

       190        200        210        220        230        240 
EVKINSKEIG GDWVIQKADG MPTYNFAVVI DDHLMEITHV LRGDDHVSNT PKQLMVYEAF 

       250        260        270        280        290        300 
GWKAPAFGHM ALIIKAETGK KLSKRDEDTL QFIEQYRELG YLPEAMFNFI GLLGWSPVGE 

       310        320        330        340        350        360 
NEIFTREQFK EMFDENRFTK ANAKFDAKKL AWVNNQWMRS EKEKVMPQLI HELVKAGLIN 

       370        380        390        400        410        420 
DQQAKNDADH LAKIIEIAGV DGIKATSEIA PLAEYPFFKL HDISKEDRQS WLETDDGQKV 

       430        440        450        460        470        480 
AAAFTEKIKA LAEDEFKAGH ILQIIRDLQH DLQIKGRPLW NPIRLITTHE VQGPNLPEIL 

       490 
AVMGKDWTIK NIQLTVAV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000411 Genomic DNA. Translation: ABJ56297.1.
RefSeqYP_809962.1. NC_008528.1.

3D structure databases

ProteinModelPortalQ04GX5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203123.OEOE_0321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ56297; ABJ56297; OEOE_0321.
GeneID4416247.
KEGGooe:OEOE_0321.
PATRIC22798773. VBIOenOen113004_0333.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK09698.
OMALMLFGRD.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycOOEN203123:GHNL-321-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_OENOB
AccessionPrimary (citable) accession number: Q04GX5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 14, 2006
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries