Reviewed, UniProtKB/Swiss-Prot Q04GD9 (GUAC_OENOB)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	OEOE_0535

Organism

Oenococcus oeni (strain BAA-331 / PSU-1) [Complete proteome] [HAMAP]

Taxonomic identifier

203123 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Oenococcus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	GMP reductase		PRO_0000294279
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04GD9-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: D9ABC3A38DF9D89C	FASTA	323	35,343
	10 20 30 40 50 60 MEVFDYENVQ LIPNKCLISS RSEADTSVEF GGHRFKLPVV PANMASVIDD KLAIWLAENG 70 80 90 100 110 120 YFYIMHRFEP GKRFNFVTDM KQRGLISSIS VGVKEEEYRL IDELVDAGLT PDYITIDIAH 130 140 150 160 170 180 GYANTVIDMI HYIKKHLPKA FVVAGNIATP DAVRELEDAG ADATKVGIGP GRACITKLKT 190 200 210 220 230 240 GFGTAGWQLA AVRLCAKAAR KPIIADGGIR HNGDIAKSVR FGASMVMIGS LFAGHKQSPG 250 260 270 280 290 300 SDLVIDHRHY KQYYGSASAK QKGVYKNVEG KDLLVPYRGD IANTLNEMAQ DLQSSISYAG 310 320 GNNLQALRTV NYVIVQNTIM NGD

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000411 Genomic DNA. Translation: ABJ56483.1.
RefSeq	YP_810148.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4416658.
GenomeReviews	Gene locus OEOE_0535 in contig CP000411_GR.
KEGG	ooe:OEOE_0535.
NMPDR	fig|203123.1.peg.1452.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

GUAC_OENOB

Accession

Primary (citable) accession number: Q04GD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 14, 2006
Last modified:	July 22, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)