Q04FS6 (FTHS_OENOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	OEOE_0769

Organism

Oenococcus oeni (strain ATCC BAA-331 / PSU-1) [Complete proteome] [HAMAP]

Taxonomic identifier

203123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Leuconostocaceae › Oenococcus › Oenococcus oeni

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000300532
Regions
	Nucleotide binding	63 – 70	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04FS6 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 5CDF80AAA4DA3EEF	FASTA	553	59,483
	10 20 30 40 50 60 MKSDIEIDHS IKALPITEIG KQIGLSDSQL IPYGHDKAKI DAYSIANMPR QGKLVLVTSI 70 80 90 100 110 120 NPTPAGEGKT TVTIGLVDAI NRLGKSAIGA LREPSMGPVF GLKGGATGGG YAQVIPMEDI 130 140 150 160 170 180 NLHFTGDIHA VSAAHNLLAA VIDNHLHQGN ELKIDPENIY WRRVLDMNDR ALRQITLGKG 190 200 210 220 230 240 RVNGPERNSG FDITASSEIM AVLTLSKNLF DLKKRLSRIV VALDVQGKPV TVADLKVAGA 250 260 270 280 290 300 LTAILKDAIN PNLVQSLEHS PFIIHGGPFA NIAQGTNSVV ATDAALKLAD FAVTEAGFGS 310 320 330 340 350 360 DLGGEKFMDV KVPVLGKEPD AVVIVATVKA LKFHGGVALD HLSDKNVDAV RNGLDNLNRH 370 380 390 400 410 420 LEAMTHYGKP VVVALNKFLD DDMEEIQLIK NFVEGEKKLQ FEIVTSFVDG FEGSLDLAKK 430 440 450 460 470 480 VIEATDNQRF FMPLYQADDS IENKIQTIVE KIYGGKDFEL SDRAKKDLAE VKENGWGKLP 490 500 510 520 530 540 VVIAKTPNSL TDDSKIHGAP TGFTIHIRRF IPKIGAGFIV AMAGKVLMMP GLGKNPAAEK 550 IDVDENGKIS GLS

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-331 / PSU-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000411 Genomic DNA. Translation: ABJ56696.1.
RefSeq	YP_810361.1. NC_008528.1.
3D structure databases
ProteinModelPortal	Q04FS6.
SMR	Q04FS6. Positions 4-551.
ModBase	Search...
Protein-protein interaction databases
STRING	203123.OEOE_0769.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ56696; ABJ56696; OEOE_0769.
GeneID	4416536.
KEGG	ooe:OEOE_0769.
PATRIC	22799697. VBIOenOen113004_0783.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
Enzyme and pathway databases
BioCyc	OOEN203123:GHNL-804-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_OENOB

Accession

Primary (citable) accession number: Q04FS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program