Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q04FL2 (SYL_OENOB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:OEOE_0839
OrganismOenococcus oeni (strain ATCC BAA-331 / PSU-1) [Complete proteome] [HAMAP]
Taxonomic identifier203123 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesOenococcus

Protein attributes

Sequence length810 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABJ56760.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 810810Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334785

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif582 – 5865"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5851ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04FL2 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 69B5134C35CB569A

FASTA81093,102
        10         20         30         40         50         60 
MSYDHKAIEK RWQTYWDEHK TFKTAPETSG KPKYYVMNMF PYPSGQGLHV GHPESYTATD 

        70         80         90        100        110        120 
IMARFNRMRG FNVLQPMGWD AFGLPAEQYA IKTGHNPADF TNENIKHFKK QVKSLGFSYD 

       130        140        150        160        170        180 
WDREINTTDP EYYKWTQWIF EQLYKKGLAY EDEIMVNWAP DFPGGGIVVA NEEVIDGKTE 

       190        200        210        220        230        240 
RGSYPVYRVP MKQWVLRITK YAERLLSDLD DLDWPEAIKE QQRNWIGKST GAAVFFKVDG 

       250        260        270        280        290        300 
KSDYQVEVYT TRPDTLFGAS YMVLAPEHDL VDKITTSECR ADVDAYKAKI ASKSDLERTD 

       310        320        330        340        350        360 
LNREKTGAFT GAYGINPVNG EKIPIWIADY VLSSYGTGAI MAVPAHDTRD YEFAKKFDLP 

       370        380        390        400        410        420 
IRQVIKGGDI SKEAFTGDGT HINSAFLDVL DKKQAIEKII DWLEEHDAGH KQVNYKLRDW 

       430        440        450        460        470        480 
IFSRQRYWGE PIPVIHWEDG KQTLVPESEL PLRLPHLNQD QMKPSGTGES PLANAKSWLE 

       490        500        510        520        530        540 
VTRQDGVKGR RETNTMPQWA GSSWYFLRYI DPHNDKVLAD PEKLKYWMNV DLYVGGAEHA 

       550        560        570        580        590        600 
VLHLLYARFW HKFLYDLGIV PTKEPFQKLV NQGMILGTNH EKMSKSKGNV VNPDEIVDSY 

       610        620        630        640        650        660 
GADALRVYEM FMGPLTQSKP WSTESLAGIR RYLDRVWRIF TSDGDGINPI IVSENDHKLD 

       670        680        690        700        710        720 
KIFNQTVKKV TEDYAAMRFN TAISQMMVFI NETFKAEKLP KKYMNAFLQL LNPVAPHITE 

       730        740        750        760        770        780 
ELWQRMGHQE TIAYATWPTY DESQIIEKQI EMAVQINGKV RAKINTPVDI SRDELAKMAT 

       790        800        810 
DNPDVKKQVA DKNIVKIIAI PGKIINIVVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000411 Genomic DNA. Translation: ABJ56760.1. Different initiation.
RefSeqYP_810425.1. NC_008528.1.

3D structure databases

ProteinModelPortalQ04FL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203123.OEOE_0839.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ56760; ABJ56760; OEOE_0839.
GeneID4416877.
KEGGooe:OEOE_0839.
PATRIC22799851. VBIOenOen113004_0858.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycOOEN203123:GHNL-839-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_OENOB
AccessionPrimary (citable) accession number: Q04FL2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries