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Q04F24

- BGAL_OENOB

UniProt

Q04F24 - BGAL_OENOB

Protein

Beta-galactosidase

Gene

lacZ

Organism
Oenococcus oeni (strain ATCC BAA-331 / PSU-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei98 – 981SubstrateUniRule annotation
    Metal bindingi197 – 1971SodiumUniRule annotation
    Binding sitei197 – 1971SubstrateUniRule annotation
    Sitei353 – 3531Transition state stabilizerUniRule annotation
    Sitei387 – 3871Transition state stabilizerUniRule annotation
    Metal bindingi412 – 4121Magnesium 1UniRule annotation
    Metal bindingi414 – 4141Magnesium 1UniRule annotation
    Active sitei457 – 4571Proton donorUniRule annotation
    Metal bindingi457 – 4571Magnesium 1UniRule annotation
    Binding sitei457 – 4571SubstrateUniRule annotation
    Active sitei533 – 5331NucleophileUniRule annotation
    Metal bindingi593 – 5931Magnesium 2UniRule annotation
    Metal bindingi597 – 5971Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi600 – 6001SodiumUniRule annotation
    Binding sitei600 – 6001SubstrateUniRule annotation
    Binding sitei1005 – 10051SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciOOEN203123:GHNL-1044-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:OEOE_1044
    OrganismiOenococcus oeni (strain ATCC BAA-331 / PSU-1)
    Taxonomic identifieri203123 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus
    ProteomesiUP000000774: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10311031Beta-galactosidasePRO_0000367004Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203123.OEOE_1044.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04F24.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni533 – 5364Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiCHGFESI.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04F24-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNKISFRDI INRKDWENPV ITNWHRLPIH TEMNYSKSLN EDKQKTIQSL     50
    NGNWCFSYFS KVTDVPENWA DRDLTKSNIM PVPSNWQLHG YDQPIYSNVA 100
    YPFPANPPYL PEENPTACYS RIFQLNDDWL QSGQNHVIFN GVGSAFHLWL 150
    NGQWIGYSED SRLPAEFDLT KYLKSGKNRI SVMVLRWSKG SYFEDQDMWR 200
    MSGIFRDVEV KHLPATYLQD YQLQTDLDDD LDQAKITIKA QVAGKNFSQN 250
    KLRTRLYFAN EKVADQSSRL STRAVDERGP LDNQFIAELN LKDPYLWSAE 300
    LPYLYQLVIE LLTDDGDILQ VEKVNIGVRK VEIKNGLLKL NGKPLLIRGT 350
    NKHEFDSKKG YAVDEETMIQ DIKAMKRNNF NAVRCSHYPN NRRWYELCDQ 400
    YGLYVVDEAN IETHGMVPMN RLTNDPVYLP LMSDRVTRMV TRDRNHPSII 450
    IWSLGNESGY GRNHAALYNW IKQSDLSRPV QYEGGGANTA VTDIIVPMYA 500
    RVEQDQIESV NSKWSLKKWI GLPGETRPLI LCEYAHDMGN SLGGFGKYWQ 550
    AFHKYPRLQG GFIWDWVDQG LLKKDVNGND FYAYGGDFKD QPNDRQFCLD 600
    GLLFPDRTPK PAMHEVKYWQ QYYLFNLQRN PLGQAESFTV TNDYSFKKSS 650
    NERLHYQIKS ENEIVIDKYI DLVLNPGESL LIKLPKGRSS TSSLLDIDIS 700
    LIKGNSWAPS GFKIASEQYV LAKKFGPTNA VTAATNKISL IENKDTNTFE 750
    IKLDDQKWQF AKNSGLLVSW SKSGNENLLD ALRDQFTRAP LDNDIGVSKV 800
    DHIDPNAWYE RWKSAGMYNL KTNLVSIDAE QLERAVLIRT EHSYSNHFQI 850
    LFKSSKIYRI DANGTMTVTV DVSLAQGIPF PARIGLTCHL ADQITDVSYT 900
    GLGPFENYPD RQSAAQYGHW QMELDDLYTP YIFPSENGSR GQVSQLEFGK 950
    QKISAYHEQN FSFNLSRFSK QQLARISHRN LLQAENGVWL SIDGYRMGVG 1000
    GDDSWSPSVA PEYLLSNNYY HYAFQWCRKD I 1031
    Length:1,031
    Mass (Da):118,758
    Last modified:November 14, 2006 - v1
    Checksum:iABBC52BF9A20F31B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000411 Genomic DNA. Translation: ABJ56948.1.
    RefSeqiYP_810613.1. NC_008528.1.

    Genome annotation databases

    EnsemblBacteriaiABJ56948; ABJ56948; OEOE_1044.
    GeneIDi4415716.
    KEGGiooe:OEOE_1044.
    PATRICi22800265. VBIOenOen113004_1059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000411 Genomic DNA. Translation: ABJ56948.1 .
    RefSeqi YP_810613.1. NC_008528.1.

    3D structure databases

    ProteinModelPortali Q04F24.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203123.OEOE_1044.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ56948 ; ABJ56948 ; OEOE_1044 .
    GeneIDi 4415716.
    KEGGi ooe:OEOE_1044.
    PATRICi 22800265. VBIOenOen113004_1059.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi CHGFESI.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci OOEN203123:GHNL-1044-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-331 / PSU-1.

    Entry informationi

    Entry nameiBGAL_OENOB
    AccessioniPrimary (citable) accession number: Q04F24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3