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Q04F24 (BGAL_OENOB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:OEOE_1044
OrganismOenococcus oeni (strain ATCC BAA-331 / PSU-1) [Complete proteome] [HAMAP]
Taxonomic identifier203123 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus

Protein attributes

Sequence length1031 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10311031Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367004

Regions

Region533 – 5364Substrate binding By similarity

Sites

Active site4571Proton donor By similarity
Active site5331Nucleophile By similarity
Metal binding1971Sodium By similarity
Metal binding4121Magnesium 1 By similarity
Metal binding4141Magnesium 1 By similarity
Metal binding4571Magnesium 1 By similarity
Metal binding5931Magnesium 2 By similarity
Metal binding5971Sodium; via carbonyl oxygen By similarity
Metal binding6001Sodium By similarity
Binding site981Substrate By similarity
Binding site1971Substrate By similarity
Binding site4571Substrate By similarity
Binding site6001Substrate By similarity
Binding site10051Substrate By similarity
Site3531Transition state stabilizer By similarity
Site3871Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04F24 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: ABBC52BF9A20F31B

FASTA1,031118,758
        10         20         30         40         50         60 
MTNKISFRDI INRKDWENPV ITNWHRLPIH TEMNYSKSLN EDKQKTIQSL NGNWCFSYFS 

        70         80         90        100        110        120 
KVTDVPENWA DRDLTKSNIM PVPSNWQLHG YDQPIYSNVA YPFPANPPYL PEENPTACYS 

       130        140        150        160        170        180 
RIFQLNDDWL QSGQNHVIFN GVGSAFHLWL NGQWIGYSED SRLPAEFDLT KYLKSGKNRI 

       190        200        210        220        230        240 
SVMVLRWSKG SYFEDQDMWR MSGIFRDVEV KHLPATYLQD YQLQTDLDDD LDQAKITIKA 

       250        260        270        280        290        300 
QVAGKNFSQN KLRTRLYFAN EKVADQSSRL STRAVDERGP LDNQFIAELN LKDPYLWSAE 

       310        320        330        340        350        360 
LPYLYQLVIE LLTDDGDILQ VEKVNIGVRK VEIKNGLLKL NGKPLLIRGT NKHEFDSKKG 

       370        380        390        400        410        420 
YAVDEETMIQ DIKAMKRNNF NAVRCSHYPN NRRWYELCDQ YGLYVVDEAN IETHGMVPMN 

       430        440        450        460        470        480 
RLTNDPVYLP LMSDRVTRMV TRDRNHPSII IWSLGNESGY GRNHAALYNW IKQSDLSRPV 

       490        500        510        520        530        540 
QYEGGGANTA VTDIIVPMYA RVEQDQIESV NSKWSLKKWI GLPGETRPLI LCEYAHDMGN 

       550        560        570        580        590        600 
SLGGFGKYWQ AFHKYPRLQG GFIWDWVDQG LLKKDVNGND FYAYGGDFKD QPNDRQFCLD 

       610        620        630        640        650        660 
GLLFPDRTPK PAMHEVKYWQ QYYLFNLQRN PLGQAESFTV TNDYSFKKSS NERLHYQIKS 

       670        680        690        700        710        720 
ENEIVIDKYI DLVLNPGESL LIKLPKGRSS TSSLLDIDIS LIKGNSWAPS GFKIASEQYV 

       730        740        750        760        770        780 
LAKKFGPTNA VTAATNKISL IENKDTNTFE IKLDDQKWQF AKNSGLLVSW SKSGNENLLD 

       790        800        810        820        830        840 
ALRDQFTRAP LDNDIGVSKV DHIDPNAWYE RWKSAGMYNL KTNLVSIDAE QLERAVLIRT 

       850        860        870        880        890        900 
EHSYSNHFQI LFKSSKIYRI DANGTMTVTV DVSLAQGIPF PARIGLTCHL ADQITDVSYT 

       910        920        930        940        950        960 
GLGPFENYPD RQSAAQYGHW QMELDDLYTP YIFPSENGSR GQVSQLEFGK QKISAYHEQN 

       970        980        990       1000       1010       1020 
FSFNLSRFSK QQLARISHRN LLQAENGVWL SIDGYRMGVG GDDSWSPSVA PEYLLSNNYY 

      1030 
HYAFQWCRKD I 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000411 Genomic DNA. Translation: ABJ56948.1.
RefSeqYP_810613.1. NC_008528.1.

3D structure databases

ProteinModelPortalQ04F24.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203123.OEOE_1044.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ56948; ABJ56948; OEOE_1044.
GeneID4415716.
KEGGooe:OEOE_1044.
PATRIC22800265. VBIOenOen113004_1059.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMACHGFESI.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycOOEN203123:GHNL-1044-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_OENOB
AccessionPrimary (citable) accession number: Q04F24
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: July 9, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries