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Protein

Beta-galactosidase

Gene

lacZ

Organism
Oenococcus oeni (strain ATCC BAA-331 / PSU-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98SubstrateUniRule annotation1
Metal bindingi197SodiumUniRule annotation1
Binding sitei197SubstrateUniRule annotation1
Sitei353Transition state stabilizerUniRule annotation1
Sitei387Transition state stabilizerUniRule annotation1
Metal bindingi412Magnesium 1UniRule annotation1
Metal bindingi414Magnesium 1UniRule annotation1
Active sitei457Proton donorUniRule annotation1
Metal bindingi457Magnesium 1UniRule annotation1
Binding sitei457SubstrateUniRule annotation1
Active sitei533NucleophileUniRule annotation1
Metal bindingi593Magnesium 2UniRule annotation1
Metal bindingi597Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi600SodiumUniRule annotation1
Binding sitei600SubstrateUniRule annotation1
Binding sitei1005SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:OEOE_1044
OrganismiOenococcus oeni (strain ATCC BAA-331 / PSU-1)
Taxonomic identifieri203123 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus
Proteomesi
  • UP000000774 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670041 – 1031Beta-galactosidaseAdd BLAST1031

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi203123.OEOE_1044.

Structurei

3D structure databases

ProteinModelPortaliQ04F24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni533 – 536Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCLDGLLF.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04F24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNKISFRDI INRKDWENPV ITNWHRLPIH TEMNYSKSLN EDKQKTIQSL
60 70 80 90 100
NGNWCFSYFS KVTDVPENWA DRDLTKSNIM PVPSNWQLHG YDQPIYSNVA
110 120 130 140 150
YPFPANPPYL PEENPTACYS RIFQLNDDWL QSGQNHVIFN GVGSAFHLWL
160 170 180 190 200
NGQWIGYSED SRLPAEFDLT KYLKSGKNRI SVMVLRWSKG SYFEDQDMWR
210 220 230 240 250
MSGIFRDVEV KHLPATYLQD YQLQTDLDDD LDQAKITIKA QVAGKNFSQN
260 270 280 290 300
KLRTRLYFAN EKVADQSSRL STRAVDERGP LDNQFIAELN LKDPYLWSAE
310 320 330 340 350
LPYLYQLVIE LLTDDGDILQ VEKVNIGVRK VEIKNGLLKL NGKPLLIRGT
360 370 380 390 400
NKHEFDSKKG YAVDEETMIQ DIKAMKRNNF NAVRCSHYPN NRRWYELCDQ
410 420 430 440 450
YGLYVVDEAN IETHGMVPMN RLTNDPVYLP LMSDRVTRMV TRDRNHPSII
460 470 480 490 500
IWSLGNESGY GRNHAALYNW IKQSDLSRPV QYEGGGANTA VTDIIVPMYA
510 520 530 540 550
RVEQDQIESV NSKWSLKKWI GLPGETRPLI LCEYAHDMGN SLGGFGKYWQ
560 570 580 590 600
AFHKYPRLQG GFIWDWVDQG LLKKDVNGND FYAYGGDFKD QPNDRQFCLD
610 620 630 640 650
GLLFPDRTPK PAMHEVKYWQ QYYLFNLQRN PLGQAESFTV TNDYSFKKSS
660 670 680 690 700
NERLHYQIKS ENEIVIDKYI DLVLNPGESL LIKLPKGRSS TSSLLDIDIS
710 720 730 740 750
LIKGNSWAPS GFKIASEQYV LAKKFGPTNA VTAATNKISL IENKDTNTFE
760 770 780 790 800
IKLDDQKWQF AKNSGLLVSW SKSGNENLLD ALRDQFTRAP LDNDIGVSKV
810 820 830 840 850
DHIDPNAWYE RWKSAGMYNL KTNLVSIDAE QLERAVLIRT EHSYSNHFQI
860 870 880 890 900
LFKSSKIYRI DANGTMTVTV DVSLAQGIPF PARIGLTCHL ADQITDVSYT
910 920 930 940 950
GLGPFENYPD RQSAAQYGHW QMELDDLYTP YIFPSENGSR GQVSQLEFGK
960 970 980 990 1000
QKISAYHEQN FSFNLSRFSK QQLARISHRN LLQAENGVWL SIDGYRMGVG
1010 1020 1030
GDDSWSPSVA PEYLLSNNYY HYAFQWCRKD I
Length:1,031
Mass (Da):118,758
Last modified:November 14, 2006 - v1
Checksum:iABBC52BF9A20F31B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ56948.1.
RefSeqiWP_002821094.1. NC_008528.1.

Genome annotation databases

EnsemblBacteriaiABJ56948; ABJ56948; OEOE_1044.
GeneIDi4415716.
KEGGiooe:OEOE_1044.
PATRICi22800265. VBIOenOen113004_1059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ56948.1.
RefSeqiWP_002821094.1. NC_008528.1.

3D structure databases

ProteinModelPortaliQ04F24.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203123.OEOE_1044.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ56948; ABJ56948; OEOE_1044.
GeneIDi4415716.
KEGGiooe:OEOE_1044.
PATRICi22800265. VBIOenOen113004_1059.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCLDGLLF.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_OENOB
AccessioniPrimary (citable) accession number: Q04F24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.