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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Oenococcus oeni (strain ATCC BAA-331 / PSU-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461S-adenosyl-L-methionineUniRule annotation
Binding sitei71 – 711S-adenosyl-L-methionineUniRule annotation
Binding sitei100 – 1001S-adenosyl-L-methionineUniRule annotation
Active sitei122 – 1221By similarity
Binding sitei122 – 1221S-adenosyl-L-methionineUniRule annotation
Binding sitei126 – 1261SubstrateUniRule annotation
Binding sitei158 – 1581SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciOOEN203123:GHNL-1272-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:OEOE_1272
OrganismiOenococcus oeni (strain ATCC BAA-331 / PSU-1)
Taxonomic identifieri203123 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus
ProteomesiUP000000774 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219tRNA (guanine-N(7)-)-methyltransferasePRO_0000288193Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203123.OEOE_1272.

Structurei

3D structure databases

ProteinModelPortaliQ04EH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 1336Interaction with RNAUniRule annotation
Regioni199 – 2024Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04EH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRSKKWAV PFIDEHPDLI IKDQRAKQLK GSWSSVFGNN HPLYLEIGSG
60 70 80 90 100
KGQFILDNAI KYPENNFIGL ELQPTAVAIA GKKAFKQEPQ LANLKLILGD
110 120 130 140 150
GGDVSDYFAD NEISKIFLNH SDPWPKAKHE KRRLTAENFL KSYSKILLPN
160 170 180 190 200
GSLELKTDNL GLFEYSLSSF KKFGLSWPEE QISYDLHHEL KKNPVNIETE
210
YEKKFAEMNQ PEYWIRVNF
Length:219
Mass (Da):25,272
Last modified:November 14, 2006 - v1
Checksum:i2BBD8BD0D72C25AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ57145.1.
RefSeqiWP_002820317.1. NC_008528.1.
YP_810810.1. NC_008528.1.

Genome annotation databases

EnsemblBacteriaiABJ57145; ABJ57145; OEOE_1272.
GeneIDi4415463.
KEGGiooe:OEOE_1272.
PATRICi22800746. VBIOenOen113004_1284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ57145.1.
RefSeqiWP_002820317.1. NC_008528.1.
YP_810810.1. NC_008528.1.

3D structure databases

ProteinModelPortaliQ04EH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203123.OEOE_1272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ57145; ABJ57145; OEOE_1272.
GeneIDi4415463.
KEGGiooe:OEOE_1272.
PATRICi22800746. VBIOenOen113004_1284.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciOOEN203123:GHNL-1272-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-331 / PSU-1.

Entry informationi

Entry nameiTRMB_OENOB
AccessioniPrimary (citable) accession number: Q04EH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 14, 2006
Last modified: May 27, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.