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Q04EH7 (TRMB_OENOB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:OEOE_1272
OrganismOenococcus oeni (strain ATCC BAA-331 / PSU-1) [Complete proteome] [HAMAP]
Taxonomic identifier203123 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288193

Regions

Region128 – 1336Interaction with RNA Potential
Region199 – 2024Substrate binding By similarity

Sites

Active site1221 By similarity
Binding site461S-adenosyl-L-methionine By similarity
Binding site711S-adenosyl-L-methionine By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1221S-adenosyl-L-methionine By similarity
Binding site1261Substrate By similarity
Binding site1581Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04EH7 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 2BBD8BD0D72C25AB

FASTA21925,272
        10         20         30         40         50         60 
MRLRSKKWAV PFIDEHPDLI IKDQRAKQLK GSWSSVFGNN HPLYLEIGSG KGQFILDNAI 

        70         80         90        100        110        120 
KYPENNFIGL ELQPTAVAIA GKKAFKQEPQ LANLKLILGD GGDVSDYFAD NEISKIFLNH 

       130        140        150        160        170        180 
SDPWPKAKHE KRRLTAENFL KSYSKILLPN GSLELKTDNL GLFEYSLSSF KKFGLSWPEE 

       190        200        210 
QISYDLHHEL KKNPVNIETE YEKKFAEMNQ PEYWIRVNF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000411 Genomic DNA. Translation: ABJ57145.1.
RefSeqYP_810810.1. NC_008528.1.

3D structure databases

ProteinModelPortalQ04EH7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203123.OEOE_1272.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ57145; ABJ57145; OEOE_1272.
GeneID4415463.
KEGGooe:OEOE_1272.
PATRIC22800746. VBIOenOen113004_1284.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAINYLEAT.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycOOEN203123:GHNL-1272-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_OENOB
AccessionPrimary (citable) accession number: Q04EH7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 14, 2006
Last modified: July 9, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways