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Q04DS4

- GLMU_OENOB

UniProt

Q04DS4 - GLMU_OENOB

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Oenococcus oeni (strain ATCC BAA-331 / PSU-1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231UDP-GlcNAcUniRule annotation
Binding sitei69 – 691UDP-GlcNAcUniRule annotation
Metal bindingi97 – 971MagnesiumUniRule annotation
Binding sitei132 – 1321UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei148 – 1481UDP-GlcNAcUniRule annotation
Binding sitei163 – 1631UDP-GlcNAcUniRule annotation
Metal bindingi214 – 2141MagnesiumUniRule annotation
Binding sitei214 – 2141UDP-GlcNAcUniRule annotation
Binding sitei286 – 2861Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei304 – 3041Acetyl-CoAUniRule annotation
Active sitei316 – 3161Proton acceptorUniRule annotation
Binding sitei319 – 3191Acetyl-CoAUniRule annotation
Binding sitei330 – 3301Acetyl-CoAUniRule annotation
Binding sitei358 – 3581Acetyl-CoAUniRule annotation
Binding sitei376 – 3761Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei393 – 3931Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciOOEN203123:GHNL-1542-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:OEOE_1542
OrganismiOenococcus oeni (strain ATCC BAA-331 / PSU-1)
Taxonomic identifieri203123 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus
ProteomesiUP000000774: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Bifunctional protein GlmUPRO_1000069734Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi203123.OEOE_1542.

Structurei

3D structure databases

ProteinModelPortaliQ04DS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 216216PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni9 – 124UDP-GlcNAc bindingUniRule annotation
Regioni217 – 23721LinkerUniRule annotationAdd
BLAST
Regioni238 – 426189N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni339 – 3402Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiCNIAAGT.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04DS4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEVDVVILA AGKGSRMKDD LSKPLHKVAG LPMLEWICRA VRKFNPKNII
60 70 80 90 100
AVQGADEDFS SYVDETVVQK EQLGSADALR CAFPKIDAEK LIVINADMPL
110 120 130 140 150
MTENDLVDLV EKGEGFDAAL LTADLKKPFG YGRVIPVGER NVVEQIVEER
160 170 180 190 200
DATADQKKLH LVNAGVYLFR ADYIKRAINN VTTDNSQSEY YLTDALPGAK
210 220 230 240 250
IVQVADWHDI LGVNTQQQLA AVSKIARKRI NDQIMANGVT MIDPLTTYID
260 270 280 290 300
ANVLVGTGTI IKPGTVIEHD SVIGAENEIG PYAHLREKTV TGIDVHIGNF
310 320 330 340 350
VETKNAKIGD HTHIGHLTYV GDAEVGQAVN IGAGTIFVNY DGKNKHMTKV
360 370 380 390 400
GDRAFIGSNS KLVAPVEIAS EAITAAGSTI TDNVDQHAMG IARQRQTNKS
410 420
DFWQRMPHED FATEYDAKHD QRDDQP
Length:426
Mass (Da):46,641
Last modified:November 14, 2006 - v1
Checksum:iCCDF7323AEBC1E01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ57398.1.
RefSeqiYP_811063.1. NC_008528.1.

Genome annotation databases

EnsemblBacteriaiABJ57398; ABJ57398; OEOE_1542.
GeneIDi4415811.
KEGGiooe:OEOE_1542.
PATRICi22801324. VBIOenOen113004_1568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ57398.1 .
RefSeqi YP_811063.1. NC_008528.1.

3D structure databases

ProteinModelPortali Q04DS4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203123.OEOE_1542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ57398 ; ABJ57398 ; OEOE_1542 .
GeneIDi 4415811.
KEGGi ooe:OEOE_1542.
PATRICi 22801324. VBIOenOen113004_1568.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi CNIAAGT.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci OOEN203123:GHNL-1542-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-331 / PSU-1.

Entry informationi

Entry nameiGLMU_OENOB
AccessioniPrimary (citable) accession number: Q04DS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 14, 2006
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3