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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Oenococcus oeni (strain ATCC BAA-331 / PSU-1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23UDP-GlcNAcUniRule annotation1
Binding sitei69UDP-GlcNAcUniRule annotation1
Metal bindingi97MagnesiumUniRule annotation1
Binding sitei132UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei148UDP-GlcNAcUniRule annotation1
Binding sitei163UDP-GlcNAcUniRule annotation1
Metal bindingi214MagnesiumUniRule annotation1
Binding sitei214UDP-GlcNAcUniRule annotation1
Binding sitei286UDP-GlcNAcUniRule annotation1
Binding sitei304UDP-GlcNAcUniRule annotation1
Active sitei316Proton acceptorUniRule annotation1
Binding sitei319UDP-GlcNAcUniRule annotation1
Binding sitei330UDP-GlcNAcUniRule annotation1
Binding sitei333Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei358Acetyl-CoAUniRule annotation1
Binding sitei376Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei393Acetyl-CoAUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:OEOE_1542
OrganismiOenococcus oeni (strain ATCC BAA-331 / PSU-1)
Taxonomic identifieri203123 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeOenococcus
Proteomesi
  • UP000000774 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000697341 – 426Bifunctional protein GlmUAdd BLAST426

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi203123.OEOE_1542.

Structurei

3D structure databases

ProteinModelPortaliQ04DS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 216PyrophosphorylaseUniRule annotationAdd BLAST216
Regioni9 – 12UDP-GlcNAc bindingUniRule annotation4
Regioni217 – 237LinkerUniRule annotationAdd BLAST21
Regioni238 – 426N-acetyltransferaseUniRule annotationAdd BLAST189
Regioni339 – 340Acetyl-CoA bindingUniRule annotation2

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CAJ. Bacteria.
COG1207. LUCA.
HOGENOMiHOG000283476.
KOiK04042.
OMAiFAHARPK.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU. 1 hit.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep.
IPR025877. MobA-like_NTP_Trfase.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04DS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVDVVILA AGKGSRMKDD LSKPLHKVAG LPMLEWICRA VRKFNPKNII
60 70 80 90 100
AVQGADEDFS SYVDETVVQK EQLGSADALR CAFPKIDAEK LIVINADMPL
110 120 130 140 150
MTENDLVDLV EKGEGFDAAL LTADLKKPFG YGRVIPVGER NVVEQIVEER
160 170 180 190 200
DATADQKKLH LVNAGVYLFR ADYIKRAINN VTTDNSQSEY YLTDALPGAK
210 220 230 240 250
IVQVADWHDI LGVNTQQQLA AVSKIARKRI NDQIMANGVT MIDPLTTYID
260 270 280 290 300
ANVLVGTGTI IKPGTVIEHD SVIGAENEIG PYAHLREKTV TGIDVHIGNF
310 320 330 340 350
VETKNAKIGD HTHIGHLTYV GDAEVGQAVN IGAGTIFVNY DGKNKHMTKV
360 370 380 390 400
GDRAFIGSNS KLVAPVEIAS EAITAAGSTI TDNVDQHAMG IARQRQTNKS
410 420
DFWQRMPHED FATEYDAKHD QRDDQP
Length:426
Mass (Da):46,641
Last modified:November 14, 2006 - v1
Checksum:iCCDF7323AEBC1E01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ57398.1.
RefSeqiWP_011677759.1. NC_008528.1.

Genome annotation databases

EnsemblBacteriaiABJ57398; ABJ57398; OEOE_1542.
GeneIDi4415811.
KEGGiooe:OEOE_1542.
PATRICi22801324. VBIOenOen113004_1568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000411 Genomic DNA. Translation: ABJ57398.1.
RefSeqiWP_011677759.1. NC_008528.1.

3D structure databases

ProteinModelPortaliQ04DS4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203123.OEOE_1542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ57398; ABJ57398; OEOE_1542.
GeneIDi4415811.
KEGGiooe:OEOE_1542.
PATRICi22801324. VBIOenOen113004_1568.

Phylogenomic databases

eggNOGiENOG4105CAJ. Bacteria.
COG1207. LUCA.
HOGENOMiHOG000283476.
KOiK04042.
OMAiFAHARPK.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU. 1 hit.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep.
IPR025877. MobA-like_NTP_Trfase.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLMU_OENOB
AccessioniPrimary (citable) accession number: Q04DS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 14, 2006
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.