Q04D35 (CAPPA_OENOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppcA
Ordered Locus Names:	OEOE_1798

Organism

Oenococcus oeni (strain ATCC BAA-331 / PSU-1) [Complete proteome] [HAMAP]

Taxonomic identifier

203123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Leuconostocaceae › Oenococcus › Oenococcus oeni

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_01904
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_01904
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the PEPCase type 2 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904		PRO_0000309597

Sequences

	Sequence		Length	Mass (Da)
	Q04D35 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 67CFCE84756E8994	FASTA	507	58,114
	10 20 30 40 50 60 MHKIDRKIPN IMGTQHPDNA GVPFFKGDQS KNPFITAYKE VDEAFNNFAV LDTDEYMWDW 70 80 90 100 110 120 EGKHADAAVI DRLYSEHYEY FKGHQLGRDK FLTFRLPNIW EEKGYSLMQA MTTMLSAEDF 130 140 150 160 170 180 ARDLSFEQRP LFEAILPMAQ TSEQLIEIED KFSKLAHFKS DEFTSGSNNT DHIEMIPLFE 190 200 210 220 230 240 GFKTQLHSPE ILKEYLQLYE QRTGQSLPYL RVFLAGSDSA LSNGFMNSII GNKLALTRLH 250 260 270 280 290 300 EFSDQKDLPI FPIAGTGSTI FRGGLSPKWI KRYLQEFPGL KTATVQSAFR YDYPLKQVQS 310 320 330 340 350 360 AIKELRSGLQ NNQAVSMTDR EQNVLINVAK KSADCYHETL DQLINDLQKI FDAFPKRRDR 370 380 390 400 410 420 RQHVGILGYS RQVDGYKMPR AITFTGSLYS VGVPPEFIGF GRALMSLNAE ELAIFTRHYP 430 440 450 460 470 480 NLRNDFTFLA HYISVDALES LIAKNPAWLE AKKDIQDLQS ILQFEIGPSN EKEEEHSRLA 490 500 SDLVKISDPT TRTLLIQKQA VLRNFLG

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-331 / PSU-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000411 Genomic DNA. Translation: ABJ57637.1.
RefSeq	YP_811302.1. NC_008528.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	203123.OEOE_1798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ57637; ABJ57637; OEOE_1798.
GeneID	4416456.
KEGG	ooe:OEOE_1798.
PATRIC	22801868. VBIOenOen113004_1838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1892.
HOGENOM	HOG000038601.
KO	K01595.
OMA	MCTQHPD.
ProtClustDB	PRK13655.
Enzyme and pathway databases
BioCyc	OOEN203123:GHNL-1802-MONOMER.
Family and domain databases
HAMAP	MF_01904. PEPcase_type2.
InterPro	IPR007566. PEP_COase_arc-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR02751. PEPCase_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPPA_OENOB

Accession

Primary (citable) accession number: Q04D35

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program