Reviewed, UniProtKB/Swiss-Prot Q04CP9 (LUXS1_LACDB)

Names and origin

Protein names

Recommended name:
    S-ribosylhomocysteine lyase 1
    EC=4.4.1.21
Alternative name(s):
    Autoinducer-2 production protein luxS 1
    AI-2 synthesis protein 1

Gene names

Name:	luxS1
Ordered Locus Names:	LBUL_0088

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365) [Complete proteome] [HAMAP]

Taxonomic identifier

321956 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity.
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the luxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	S-ribosylhomocysteine lyase 1 HAMAP MF_00091		PRO_0000298007
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	124	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04CP9-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 3821FCA8F57568AD	FASTA	159	17,859
	10 20 30 40 50 60 MAKVESFELD HTKVKAPYVR LITVEEGPKG DKISNFDLRL VQPNENAIPT GGLHTIEHLL 70 80 90 100 110 120 ASLLRDRLDG VIDCSPFGCR TGFHLITWGE HSTTEVAKAL RDSLKAIRDD ITWEDVPGTT 130 140 150 IESCGNYRDH SLFSAQEWCN DILKKGISDD PFDRHVVED

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000412 Genomic DNA. Translation: ABJ57773.1.
RefSeq	YP_812211.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q04CP9.
Genome annotation databases
GeneID	4436394.
GenomeReviews	Gene locus LBUL_0088 in contig CP000412_GR.
KEGG	lbu:LBUL_0088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YFGPMGC.
Family and domain databases
HAMAP	MF_00091. [Tree]
InterPro	IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. LuxS. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

LUXS1_LACDB

Accession

Primary (citable) accession number: Q04CP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	November 14, 2006
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)