Q04BW7 (Q04BW7_LACDB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase RuleBase RU003812 HAMAP MF_00193
EC=6.3.1.5 RuleBase RU003812 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	LBUL_0405

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365) [Complete proteome] [HAMAP]

Taxonomic identifier

321956 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. RuleBase RU003812 HAMAP MF_00193 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 EMBL ABJ58055.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	49	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	Q04BW7 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 49D1D0EE5438228E	FASTA	276	30,875
	10 20 30 40 50 60 MRELQKKIVE YEHVLPEIDP QEEIRKTVDF LKDYLKANPF LKSYVLGISG GQDSTLTGKL 70 80 90 100 110 120 TQMAISEMRE ETGDDSYKFI AVRLPYGVQA DAADAADAVA FIQPDVDLIE NIKPATDAMV 130 140 150 160 170 180 ATLKETGVEM TDFNKGNIKA RQRMVMQYAI AGANQGAVVG TDHAAENFSG FYTKYGDGAA 190 200 210 220 230 240 DLTPLFRLDK RQGKQLLAAL GCPKHLYLKA PTADLEEEKP SLPDEVALGV TYEEIDDYLE 250 260 270 GREVSEKAAE RIEELWTKSE HKRHLPVTIF DDYYKK

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000412 Genomic DNA. Translation: ABJ58055.1.
RefSeq	YP_812493.1. NC_008529.1.
3D structure databases
ProteinModelPortal	Q04BW7.
SMR	Q04BW7. Positions 4-276.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04BW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4435555.
GenomeReviews	Gene locus LBUL_0405 in contig CP000412_GR.
KEGG	lbu:LBUL_0405.
PATRIC	22220641. VBILacDel70259_0403.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q04BW7_LACDB

Accession

Primary (citable) accession number: Q04BW7

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)