Q04BL3 (PPNK_LACDB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	LBUL_0520

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365) [Complete proteome] [HAMAP]

Taxonomic identifier

321956 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP MF_00361
Subcellular location	Cytoplasm By similarity HAMAP MF_00361.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_1000059872

Sequences

	Sequence		Length	Mass (Da)
	Q04BL3 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 6D8C4FD756B24626	FASTA	265	29,730
	10 20 30 40 50 60 MKVAIVHNDR VTTQVAVRHL QVLLAEKGIL QDQQHPDLVI SVGGDGTLIS AFHKYKQQLD 70 80 90 100 110 120 KVCFAGIHTG HLGFYTDWRN YDMEKLVDAL ASHPVEENEV GYPLLDMKVT TSCGEKRFLA 130 140 150 160 170 180 LNEASIKRIS KTMEAEVWLG GERFENFRGD GLCVSTPTGS TAYSKSLGGA VIHPRLKTLQ 190 200 210 220 230 240 LTEIASINNL VFRTVGSPIV IAPDEWITIV PKISDRVVVI VDGERISLTD VQKVDYKIAA 250 260 EEIRFYQYGH HHFWERVNDA FIGDR

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000412 Genomic DNA. Translation: ABJ58159.1.
RefSeq	YP_812597.1. NC_008529.1.
3D structure databases
ProteinModelPortal	Q04BL3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04BL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4436624.
GenomeReviews	Gene locus LBUL_0520 in contig CP000412_GR.
KEGG	lbu:LBUL_0520.
PATRIC	22220875. VBILacDel70259_0518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HBG314020.
OMA	TISFANC.
ProtClustDB	PRK04885.
Enzyme and pathway databases
BioCyc	LDEL321956:LBUL_0520-MONOMER.
Family and domain databases
HAMAP	MF_00361. NAD_kinase. [Tree]
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. polyP/ATP_NADK_prd. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
KO	K00858.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_LACDB

Accession

Primary (citable) accession number: Q04BL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program