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Q04B65

- SYI_LACDB

UniProt

Q04B65 - SYI_LACDB

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LBUL_0681
Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylate By similarity
Binding sitei600 – 6001ATP By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity
Metal bindingi913 – 9131Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLDEL321956:GI15-679-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LBUL_0681
OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Taxonomic identifieri321956 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000665: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022084Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi321956.LBUL_0681.

Structurei

3D structure databases

ProteinModelPortaliQ04B65.
SMRiQ04B65. Positions 1-919.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi597 – 6015"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04B65-1 [UniParc]FASTAAdd to Basket

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MRVKDTLNLG KTKFPMRGNL PKREAEWEKN WEDQKFYERR LKLNEGHERF    50
DLHDGPPFAN GNIHMGHALN KITKDIIVRS KNMEGYYAPY VPGWDTHGLP 100
IEQQLTKQGV DRKTMDRAAY RELCRKFAME QVEKQRTDFK RLGVMGDWDH 150
PYITLLPEFE AAEIRVFGKM YENGYIYQGK KPVYWSWSSE STLAEAEVEY 200
HDVESPSIYI SFPVKDGKGK LSEENTYFLI WTTTPWTIPS NQGIAVNPKF 250
DYSVVEVGDR RYVVGTDRLS AVAEILGWDS YKTVQHLKGT DMEYMVAKHP 300
YIEGRDSLLM EAVYVTDDDG TGLVHTASGF GEDDYNTAMR YGFDVLSPMD 350
NKGCFTEEIP DPDLVGKFYT DTNEIVKDKL SAAGNLLHYS TFVHSAAHDW 400
RTKKPVVYRA TTQWFASISK FRDQILDQIE KTTFYPAWGK TRLYNMIKDR 450
GDWVISRQRA WGVPLPIFYA EDGTAIVTHE TIEHVADLFA KEGSNAWFTH 500
PVEELLPEGF TSEHSPNGKF TKETDILDVW FDSGSSWSGV QALGRAVHYP 550
TSMYLEGSDQ YRGWFNSSLI TSVATNGVAP YKSVLSQGFT LDGQGRKMSK 600
SLGNTIAPND VIKQMGAEII RLWVASVDAS GDVGVSMDIL RQVSEGYRKI 650
RNTFRYMLAN TADFDPEKDR VAYKDLRKID QYLEVKLNDL VAESIVNYDK 700
YDFADVYKLV FKFITNDLSA FYLDFAKDVL YIEGKDSHAR RSMQTVIYDA 750
AVKLAKILAP ILPHTMGEVW GYLKEKEEDV YLSNFPEIED YADADDLKES 800
WGEFMKLRDD VLKALEEARD QKLIGKSFEA SVTVYPGEAA KAALDKLAGE 850
DFREILIVSN LVMGQGEVPA EAKQFDQASV LVRRAEGEVC PRCRMYRTDL 900
GADSRLPQLC GRCASIVAGD HPEILEEGLE D 931
Length:931
Mass (Da):106,004
Last modified:November 14, 2006 - v1
Checksum:iC46C1DD7511A2F97
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000412 Genomic DNA. Translation: ABJ58307.1.
RefSeqiYP_812745.1. NC_008529.1.

Genome annotation databases

EnsemblBacteriaiABJ58307; ABJ58307; LBUL_0681.
GeneIDi4435874.
KEGGilbu:LBUL_0681.
PATRICi22221209. VBILacDel70259_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000412 Genomic DNA. Translation: ABJ58307.1 .
RefSeqi YP_812745.1. NC_008529.1.

3D structure databases

ProteinModelPortali Q04B65.
SMRi Q04B65. Positions 1-919.
ModBasei Search...

Protein-protein interaction databases

STRINGi 321956.LBUL_0681.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ58307 ; ABJ58307 ; LBUL_0681 .
GeneIDi 4435874.
KEGGi lbu:LBUL_0681.
PATRICi 22221209. VBILacDel70259_0682.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LDEL321956:GI15-679-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-365.

Entry informationi

Entry nameiSYI_LACDB
AccessioniPrimary (citable) accession number: Q04B65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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