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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
Binding sitei600 – 6001ATPUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLDEL321956:GI15-679-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LBUL_0681
OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Taxonomic identifieri321956 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000665: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligasePRO_1000022084Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi321956.LBUL_0681.

Structurei

3D structure databases

ProteinModelPortaliQ04B65.
SMRiQ04B65. Positions 1-919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi597 – 6015"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04B65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKDTLNLG KTKFPMRGNL PKREAEWEKN WEDQKFYERR LKLNEGHERF
60 70 80 90 100
DLHDGPPFAN GNIHMGHALN KITKDIIVRS KNMEGYYAPY VPGWDTHGLP
110 120 130 140 150
IEQQLTKQGV DRKTMDRAAY RELCRKFAME QVEKQRTDFK RLGVMGDWDH
160 170 180 190 200
PYITLLPEFE AAEIRVFGKM YENGYIYQGK KPVYWSWSSE STLAEAEVEY
210 220 230 240 250
HDVESPSIYI SFPVKDGKGK LSEENTYFLI WTTTPWTIPS NQGIAVNPKF
260 270 280 290 300
DYSVVEVGDR RYVVGTDRLS AVAEILGWDS YKTVQHLKGT DMEYMVAKHP
310 320 330 340 350
YIEGRDSLLM EAVYVTDDDG TGLVHTASGF GEDDYNTAMR YGFDVLSPMD
360 370 380 390 400
NKGCFTEEIP DPDLVGKFYT DTNEIVKDKL SAAGNLLHYS TFVHSAAHDW
410 420 430 440 450
RTKKPVVYRA TTQWFASISK FRDQILDQIE KTTFYPAWGK TRLYNMIKDR
460 470 480 490 500
GDWVISRQRA WGVPLPIFYA EDGTAIVTHE TIEHVADLFA KEGSNAWFTH
510 520 530 540 550
PVEELLPEGF TSEHSPNGKF TKETDILDVW FDSGSSWSGV QALGRAVHYP
560 570 580 590 600
TSMYLEGSDQ YRGWFNSSLI TSVATNGVAP YKSVLSQGFT LDGQGRKMSK
610 620 630 640 650
SLGNTIAPND VIKQMGAEII RLWVASVDAS GDVGVSMDIL RQVSEGYRKI
660 670 680 690 700
RNTFRYMLAN TADFDPEKDR VAYKDLRKID QYLEVKLNDL VAESIVNYDK
710 720 730 740 750
YDFADVYKLV FKFITNDLSA FYLDFAKDVL YIEGKDSHAR RSMQTVIYDA
760 770 780 790 800
AVKLAKILAP ILPHTMGEVW GYLKEKEEDV YLSNFPEIED YADADDLKES
810 820 830 840 850
WGEFMKLRDD VLKALEEARD QKLIGKSFEA SVTVYPGEAA KAALDKLAGE
860 870 880 890 900
DFREILIVSN LVMGQGEVPA EAKQFDQASV LVRRAEGEVC PRCRMYRTDL
910 920 930
GADSRLPQLC GRCASIVAGD HPEILEEGLE D
Length:931
Mass (Da):106,004
Last modified:November 14, 2006 - v1
Checksum:iC46C1DD7511A2F97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000412 Genomic DNA. Translation: ABJ58307.1.
RefSeqiYP_812745.1. NC_008529.1.

Genome annotation databases

EnsemblBacteriaiABJ58307; ABJ58307; LBUL_0681.
GeneIDi4435874.
KEGGilbu:LBUL_0681.
PATRICi22221209. VBILacDel70259_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000412 Genomic DNA. Translation: ABJ58307.1.
RefSeqiYP_812745.1. NC_008529.1.

3D structure databases

ProteinModelPortaliQ04B65.
SMRiQ04B65. Positions 1-919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi321956.LBUL_0681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ58307; ABJ58307; LBUL_0681.
GeneIDi4435874.
KEGGilbu:LBUL_0681.
PATRICi22221209. VBILacDel70259_0682.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciLDEL321956:GI15-679-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-365.

Entry informationi

Entry nameiSYI_LACDB
AccessioniPrimary (citable) accession number: Q04B65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.