Q049L7 (PURL_LACDB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	LBUL_1335

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365) [Complete proteome] [HAMAP]

Taxonomic identifier

321956 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus delbrueckii › Lactobacillus delbrueckii subsp. bulgaricus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_1000050314
Regions
	Nucleotide binding	110 – 121	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q049L7 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 0660928C36F4333F	FASTA	740	80,011
	10 20 30 40 50 60 MMQEMTPEEI KEKKPYLDWS LSEEEYDYIC DKLLHRLPNY TETGLFAAMW SEHCSYKKSK 70 80 90 100 110 120 SVLRLFPTKG PRILQGPGEG AGVVDIGDGQ AVVFKAESHN HPTAVEPYQG AATGVGGILR 130 140 150 160 170 180 DVFSMGARPV ATLDSLHFGE LDDAHTRWLL NETVAGIGGY GNCMGIPTVG GELTFDDIYK 190 200 210 220 230 240 GNPVMNAMSV GLLDVKDMQK GLAQGEGNAV MYVGAKTGRD GIHGATFASH SFDSEHESQR 250 260 270 280 290 300 SAVQVGDPFM EKLLLEACLE LTQKHADWLV GIQDMGAAGI VSSSSEMASE GKSGMELNLD 310 320 330 340 350 360 LVPQRESGMS AYEIMLSESQ ERMLLCVKKG HEEDVKKIFD FYDLDAVVIG RITSGHNYVL 370 380 390 400 410 420 RHHGEVVCDI PVTSLTEDVL EEPSEEKQPQ HMIDDAAKPA WEPEITDLAK TYKQMLAQPT 430 440 450 460 470 480 IASKGMFTQT YDSMVRTSTV VGPGEDSGVL RVRGTHKGIA MTTDGNGRFI YLDPEIGGKR 490 500 510 520 530 540 AVVEAAGNII ASGAEPLAIT DCLNFGNPNE PEVFWELHHS VMGIAKACEV LETPVVSGNV 550 560 570 580 590 600 SLYNETDGKS IYPTPMVGMV GLVKNLDHLV RDSFQEEGDD LYLVGQTGAD YAGSELQKML 610 620 630 640 650 660 TGEIFGSLND LDLDHIKDYQ KRLLAQMEAG HVASAHDLSE GGLAVSLAES SFGHGIGAEV 670 680 690 700 710 720 ACDLTSAELF SETPGRFLVS VPSEYSAEFA AALAADAVKI GQTAGDSLKL TLKDQAADLP 730 740 VAELKQIWEE ALPCLMKSKD

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000412 Genomic DNA. Translation: ABJ58855.1.
RefSeq	YP_813293.1. NC_008529.1.
3D structure databases
ProteinModelPortal	Q049L7.
ModBase	Search...
Protein-protein interaction databases
STRING	321956.LBUL_1335.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ58855; ABJ58855; LBUL_1335.
GeneID	4435743.
KEGG	lbu:LBUL_1335.
PATRIC	22222527. VBILacDel70259_1313.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	LDEL321956:GI15-1408-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_LACDB

Accession

Primary (citable) accession number: Q049L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program