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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451S-adenosyl-L-methionineUniRule annotation
Binding sitei70 – 701S-adenosyl-L-methionineUniRule annotation
Binding sitei97 – 971S-adenosyl-L-methionineUniRule annotation
Active sitei119 – 1191By similarity
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Binding sitei123 – 1231SubstrateUniRule annotation
Binding sitei155 – 1551SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLDEL321956:GI15-1411-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:LBUL_1416
OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Taxonomic identifieri321956 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218tRNA (guanine-N(7)-)-methyltransferasePRO_0000288164Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ049E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1306Interaction with RNAUniRule annotation
Regioni195 – 1984Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q049E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLKNKPWAN ELVEEHPESA LDRPNPAEKI DWAARFGNDK PIEIEVGSGK
60 70 80 90 100
GQFITTLAKQ HPDRNFVAME IQKTAAGIIL KKKLDEGLDN LQILCADAAN
110 120 130 140 150
LVAYFGENST SKIYLNFSDP WPKSRHEKRR LTYKDFLAKY QAVLTGDGLI
160 170 180 190 200
EFKTDNSGLF AYSVKSMNNY GMHFDFMSVD LHHESEEIVE KNVETEYEHK
210
FASKGQPIYC LHAGFLAK
Length:218
Mass (Da):24,814
Last modified:November 14, 2006 - v1
Checksum:iA841A875F9C25895
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000412 Genomic DNA. Translation: ABJ58926.1.
RefSeqiWP_003618358.1. NC_008529.1.
YP_813364.1. NC_008529.1.

Genome annotation databases

EnsemblBacteriaiABJ58926; ABJ58926; LBUL_1416.
KEGGilbu:LBUL_1416.
PATRICi22222691. VBILacDel70259_1394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000412 Genomic DNA. Translation: ABJ58926.1.
RefSeqiWP_003618358.1. NC_008529.1.
YP_813364.1. NC_008529.1.

3D structure databases

ProteinModelPortaliQ049E6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ58926; ABJ58926; LBUL_1416.
KEGGilbu:LBUL_1416.
PATRICi22222691. VBILacDel70259_1394.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciLDEL321956:GI15-1411-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-365.

Entry informationi

Entry nameiTRMB_LACDB
AccessioniPrimary (citable) accession number: Q049E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 14, 2006
Last modified: June 24, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.