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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLDEL321956:GI15-1446-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LBUL_1451
OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365)
Taxonomic identifieri321956 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_0000334767Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ049B7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi573 – 5775"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q049B7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNHKVVEKK WQKYWLENKT FKTGTDPEKP KYYVLDMFPY PSGKGLHVGH
60 70 80 90 100
PEGYTATDIM ARMKRAQGYN VLHPMGWDAF GLPAEQYALQ TGNDPATFTD
110 120 130 140 150
ENIAHFKKQL QALGFSYDWD REIKTTDPNY YKWTQWIFEQ MYKMGLAYEA
160 170 180 190 200
EVPVNWSPDL GTVVANEEVI DGKTERGGYP VYRRKMRQWM LKITAYADRL
210 220 230 240 250
LDDLDDLDWP EPIKEMQRNW IGRSVGAQVT FKIKDSDKSF AVFTTRPDTL
260 270 280 290 300
FGCSYTVLAP ENELVKEITS PEQKEAVDAY IKSIESKSDL ERTDLNKDKT
310 320 330 340 350
GVFTGAYAIN PVNGEEVPVW ISDYVLATYG TGAVIAVPAH DERDYAFATK
360 370 380 390 400
FDLPIKEVVE GGDISKEAFA GDGVHVNSDF LNGLHNEEAK AKMVDWLTEK
410 420 430 440 450
GVGEKKVNYK MRDWNFSRQR YWGEPIPVIH WEDGETTLVP EDELPLRLPK
460 470 480 490 500
ESNIKPSGTP ESPLANLTDW VNVVDENGRK GKRETNTMPQ WAGSSWYFLR
510 520 530 540 550
YIDPHNDKAL ADPELLKKWM PVDLYIGGAE HATLHLLYAR FWHKVLYDLG
560 570 580 590 600
VVPTKEPFQK LYNQGLILKN HEKMSKSRGN VVNPDDVVDE YGADSLRTYE
610 620 630 640 650
MFMGPLNASI DWDDNGPSGV KKFLDRVWRT FVNDLDLDPI PSEKITDKND
660 670 680 690 700
GKLDKIYNET VKTVTEHFEE LRFNTAISQM MVFMNACQKV DKIPREYAEG
710 720 730 740 750
FVKLMAPVAP HMMEEIWHVF GHDESVQFAA WPTYDASKLV ESTVEMAVTV
760 770 780 790 800
NGKKRGNFQI AKDASREEAQ AAATALPHVK EFLEGKEIKK VIVVPNKIVN

IVAK
Length:804
Mass (Da):91,939
Last modified:November 14, 2006 - v1
Checksum:iBA9ACA2304EC60F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000412 Genomic DNA. Translation: ABJ58955.1.
RefSeqiWP_011678469.1. NC_008529.1.

Genome annotation databases

EnsemblBacteriaiABJ58955; ABJ58955; LBUL_1451.
KEGGilbu:LBUL_1451.
PATRICi22222775. VBILacDel70259_1435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000412 Genomic DNA. Translation: ABJ58955.1.
RefSeqiWP_011678469.1. NC_008529.1.

3D structure databases

ProteinModelPortaliQ049B7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ58955; ABJ58955; LBUL_1451.
KEGGilbu:LBUL_1451.
PATRICi22222775. VBILacDel70259_1435.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLDEL321956:GI15-1446-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-365.

Entry informationi

Entry nameiSYL_LACDB
AccessioniPrimary (citable) accession number: Q049B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 14, 2006
Last modified: July 22, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.