Protein FMP42 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q04991 (FMP42_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein FMP42

Gene names

Name:	FMP42
Ordered Locus Names:	YMR221C
ORF Names:	YM9959.03C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.3.
Sequence similarities	Belongs to the SLC43A transporter (TC 2.A.1.44) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	fungal-type vacuole Inferred from direct assay Ref.3. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ATG27	P46989	1	EBI-27478,EBI-26164
COT1	P32798	1	EBI-27478,EBI-5006
FTH1	P38310	1	EBI-27478,EBI-20959
LSB3	P43603	1	EBI-27478,EBI-22980
PMC1	P38929	1	EBI-27478,EBI-3097
PMP2	P40975	1	EBI-27478,EBI-2043041
PMP3	P87284	1	EBI-27478,EBI-13555
VMA13	P41807	1	EBI-27478,EBI-20281
YHR146W	P38845	1	EBI-27478,EBI-24770

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

Protein FMP42

PRO_0000218642

Regions

Topological domain

1 – 11

Vacuolar Potential

Transmembrane

12 – 32

Helical; Potential

Topological domain

33 – 64

Cytoplasmic Potential

Transmembrane

65 – 85

Helical; Potential

Topological domain

86 – 91

Vacuolar Potential

Transmembrane

92 – 112

Helical; Potential

Topological domain

113 – 119

Cytoplasmic Potential

Transmembrane

120 – 140

Helical; Potential

Topological domain

141 – 150

Vacuolar Potential

Transmembrane

151 – 171

Helical; Potential

Topological domain

172 – 186

Cytoplasmic Potential

Transmembrane

187 – 207

Helical; Potential

Topological domain

208 – 302

Vacuolar Potential

Transmembrane

303 – 323

Helical; Potential

Topological domain

324 – 344

Cytoplasmic Potential

Transmembrane

345 – 365

Helical; Potential

Topological domain

366 – 385

Vacuolar Potential

Transmembrane

386 – 406

Helical; Potential

Topological domain

407 – 421

Cytoplasmic Potential

Transmembrane

422 – 442

Helical; Potential

Topological domain

443 – 462

Vacuolar Potential

Transmembrane

463 – 483

Helical; Potential

Topological domain

484 – 504

Cytoplasmic Potential

Amino acid modifications

Modified residue

236

Phosphothreonine Ref.7

Modified residue

238

Phosphoserine Ref.5 Ref.7

Modified residue

249

Phosphoserine Ref.5 Ref.7

Modified residue

269

Phosphoserine Ref.5 Ref.7

Modified residue

283

Phosphoserine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

Q04991 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 38C5E75DFB874767
Show »

FASTA

504

56,178

        10         20         30         40         50         60 
MTSTRTLRYA QVACACIWCL FSAGIIFGFA ALKPILISEG VYHELCDPKD GDRLLCTAQD 

        70         80         90        100        110        120 
LKLNFIFALS ATVTNIMALP VGKILDMYGP RVCGIIGSCL LFLASGNFIS AKHLVSLWDP 

       130        140        150        160        170        180 
YLVGYTLLAV AGPFVFISCF QLANSFPQRS GTVLALLTGS FDSSSALFLL YRLLYQNWFP 

       190        200        210        220        230        240 
TLNVSRFFTL YLIVPVFILA CQLTIMPHSS YKTVNHIAKI AVEGLDENGR LIEGDTGSGI 

       250        260        270        280        290        300 
IPDEQERQSL IAIEREEDSI PSRPQRRKSV LETYVEDKLQ KKSGGIFGVL HGKSAYEQIK 

       310        320        330        340        350        360 
SPWFYLMLLF ALVAMLRINY FIATVRTQEE YLLNDPDLAL KLNSIFDMLL PLGGAVSIPF 

       370        380        390        400        410        420 
IGLLLDHTDT LSTLTILFTT STAIGVFGLI PNSFTWNLIG IVLLVVYRPF YYTVVSDYSS 

       430        440        450        460        470        480 
KVFGFDTFGT VYGLLSCICG IFNMSQNLLD KWTHTTFNMN PFPINLTLVI LTVVFSLTLT 

       490        500 
FYIRSQILPK PVNERGLSSN YQTI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238; SER-249 AND SER-269, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-236; SER-238; SER-249 AND SER-269, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49939 Genomic DNA. Translation: CAA90192.1. BK006946 Genomic DNA. Translation: DAA10120.1.
PIR	S57589.
RefSeq	NP_013948.1. NM_001182728.1.
3D structure databases
ProteinModelPortal	Q04991.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2606N.
IntAct	Q04991. 6 interactions.
MINT	MINT-426718.
STRING	4932.YMR221C.
Proteomic databases
PaxDb	Q04991.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR221C; YMR221C; YMR221C.
GeneID	855261.
KEGG	sce:YMR221C.
Organism-specific databases
CYGD	YMR221c.
SGD	S000004834. FMP42.
Phylogenomic databases
eggNOG	NOG71084.
GeneTree	ENSGT00530000063043.
HOGENOM	HOG000171019.
OMA	INYFIAT.
OrthoDB	EOG4V9XZW.
Gene expression databases
Genevestigator	Q04991.
GermOnline	YMR221C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
ProtoNet	Search...
Other
NextBio	978855.

Entry information

Entry name

FMP42_YEAST

Accession

Primary (citable) accession number: Q04991
Secondary accession number(s): D6W046

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents