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Protein

Cyclohexadienyl dehydrogenase

Gene

tyrC

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can function as either prephenate dehydrogenase or as arogenate dehydrogenase in the biosynthesis of L-tyrosine. Catalyzes two analogous reactions: converts prephenate to 4-hydroxyphenylpyruvate and transforms L-arogenate to L-tyrosine. Is not able to utilize NADP+ instead of NAD+ as cosubstrate.1 Publication

Catalytic activityi

L-arogenate + NAD+ = L-tyrosine + NADH + CO2.1 Publication
Prephenate + NAD+ = 4-hydroxyphenylpyruvate + CO2 + NADH.1 Publication

Enzyme regulationi

Insensitive to feedback inhibition by L-tyrosine.1 Publication

Kineticsi

The KM value obtained for NAD+ is the same regardless of whether the enzyme is assayed as arogenate dehydrogenase or as prephenate dehydrogenase. Has a greater Vmax with L-arogenate as substrate (about 3-fold higher than with prephenate).1 Publication
  1. KM=0.25 mM for L-arogenate (at pH 6.6 and 37 degrees Celsius)1 Publication
  2. KM=0.18 mM for prephenate (at pH 6.6 and 37 degrees Celsius)1 Publication
  3. KM=0.09 mM for NAD+ (at pH 6.6 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.6 for both activities.1 Publication

    Pathwayi: L-tyrosine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes (4-hydroxyphenyl)pyruvate from prephenate (NAD(+) route).1 Publication
    Proteins known to be involved in this subpathway in this organism are:
    1. Cyclohexadienyl dehydrogenase (tyrC)
    This subpathway is part of the pathway L-tyrosine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (4-hydroxyphenyl)pyruvate from prephenate (NAD(+) route), the pathway L-tyrosine biosynthesis and in Amino-acid biosynthesis.

    Pathwayi: L-tyrosine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-tyrosine from L-arogenate (NAD(+) route).1 Publication
    Proteins known to be involved in this subpathway in this organism are:
    1. Cyclohexadienyl dehydrogenase (tyrC)
    This subpathway is part of the pathway L-tyrosine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tyrosine from L-arogenate (NAD(+) route), the pathway L-tyrosine biosynthesis and in Amino-acid biosynthesis.

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi6 – 30NADSequence analysisAdd BLAST25

    GO - Molecular functioni

    • cyclohexadienyl dehydrogenase activity Source: UniProtKB
    • NAD+ binding Source: UniProtKB
    • prephenate dehydrogenase (NAD+) activity Source: UniProtKB
    • prephenate dehydrogenase (NADP+) activity Source: InterPro
    • protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    • tyrosine biosynthetic process Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tyrosine biosynthesis
    LigandNAD

    Enzyme and pathway databases

    UniPathwayiUPA00122; UER00959.
    UPA00122; UER00961.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclohexadienyl dehydrogenase1 Publication
    Alternative name(s):
    Arogenate dehydrogenase1 Publication (EC:1.3.1.431 Publication)
    Short name:
    ADH1 Publication
    Prephenate dehydrogenase1 Publication (EC:1.3.1.121 Publication)
    Short name:
    PDH1 Publication
    Gene namesi
    Name:tyrC1 Publication
    Ordered Locus Names:ZMO0420
    OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
    Taxonomic identifieri264203 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
    Proteomesi
    • UP000001173 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001192091 – 293Cyclohexadienyl dehydrogenaseAdd BLAST293

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    GO - Molecular functioni

    • protein homodimerization activity Source: UniProtKB

    Structurei

    3D structure databases

    ProteinModelPortaliQ04983.
    SMRiQ04983.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 293Prephenate/arogenate dehydrogenasePROSITE-ProRule annotationAdd BLAST289

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000043493.
    KOiK00220.
    OMAiMWRDICL.

    Family and domain databases

    InterProiView protein in InterPro
    IPR008927. 6-PGluconate_DH_C-like.
    IPR036291. NAD(P)-bd_dom_sf.
    IPR003099. Prephen_DH.
    PfamiView protein in Pfam
    PF02153. PDH. 1 hit.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF51735. SSF51735. 1 hit.
    PROSITEiView protein in PROSITE
    PS51176. PDH_ADH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q04983-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVFKHIAII GLGLIGSSAA RATKAYCPDV TVSLYDKSEF VRDRARALNL
    60 70 80 90 100
    GDNVTDDIQD AVREADLVLL CVPVRAMGIV AAAMAPALKK DVIICDTGSV
    110 120 130 140 150
    KVSVIKTLQD NLPNHIIVPS HPLAGTENNG PDAGFAELFQ DHPVILTPDA
    160 170 180 190 200
    HTPAQAIAYI ADYWEEIGGR INLMSAEHHD HVLALTSHLP HVIAYQLIGM
    210 220 230 240 250
    VSGYEKKSRT PIMRYSAGSF RDATRVAASE PRLWQDIMLE NAPALLPVLD
    260 270 280 290
    HFIADLKKLR TAIASQDGDY LLEHFKESQK ARLALKTDHD IRP
    Length:293
    Mass (Da):32,051
    Last modified:February 15, 2005 - v2
    Checksum:i027683380D81146C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti42R → C in AAA27684 (PubMed:7916685).Curated1
    Sequence conflicti42R → C in CAA47647 (PubMed:7916685).Curated1
    Sequence conflicti268G → E in AAA27684 (PubMed:7916685).Curated1
    Sequence conflicti268G → E in CAA47647 (PubMed:7916685).Curated1
    Sequence conflicti292R → H in AAA27684 (PubMed:7916685).Curated1
    Sequence conflicti292R → H in CAA47647 (PubMed:7916685).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M75891 Genomic DNA. Translation: AAA27684.1.
    X67208 Genomic DNA. Translation: CAA47647.1.
    AE008692 Genomic DNA. Translation: AAV89044.1.
    PIRiS29384.
    RefSeqiWP_011240335.1. NC_006526.2.

    Genome annotation databases

    EnsemblBacteriaiAAV89044; AAV89044; ZMO0420.
    KEGGizmo:ZMO0420.

    Similar proteinsi

    Entry informationi

    Entry nameiTYRC_ZYMMO
    AccessioniPrimary (citable) accession number: Q04983
    Secondary accession number(s): Q5NQG0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 15, 2005
    Last modified: October 25, 2017
    This is version 119 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families