Q04977 (AMYM_BACLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Maltogenic alpha-amylase
EC=3.2.1.133

Alternative name(s):
Glucan 1,4-alpha-maltohydrolase

Gene names

Name:

blmA

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts starch into maltose. In contrary to other maltogenic alpha-amylases BlmA cannot hydrolyze 1,4-alpha-glucosidic linkage next to 1,6-alpha-glucosidic linkages.
Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive alpha-maltose residues from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan 1,4-alpha-maltohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Maltogenic alpha-amylase		PRO_0000054296

Sequences

	Sequence		Length	Mass (Da)
	Q04977 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F74B65F8672FAEC1	FASTA	578	66,924
	10 20 30 40 50 60 MIELAAIHHQ PFNSDAYSYN GRTLHIKIRT KKDDAEHVAW FGAILTNTPA HMESERAYVA 70 80 90 100 110 120 KIGRNKQPMI TGLPKCGLHS GSAIRIYLTA LMIETLFTEA MVHVRFRYRQ THVLNFRLFM 130 140 150 160 170 180 RQTRLMHRLG QINRLVSNFS GAFRAGGKIC SGKPLPWGRK DPEAHDFFGG HLQGIMTSWT 190 200 210 220 230 240 IWKTWGEAGI YLTPIFAAPS NHKYDTLDYC SIDPHFGDEE LFRTVVSRIH ERGMKIMLDA 250 260 270 280 290 300 VFNHIGTSQE WQDVVKNGET SRYKDWFIFI LSLLKKAAMI HLRLVPRCRS SIAGTRKFRL 310 320 330 340 350 360 ICLILRCTGS ANLISTAGVW MWQMKLIMRF GRNSGKPSPE KPDIFILGEI WHQADPWLRG 370 380 390 400 410 420 DEFHIGHELP VHRTDDSLFF RRIDFSSQIA SRINSQKMSG MKQVKEVMLN LLDSHERILT 430 440 450 460 470 480 RCGGDQRKGA RLFWHSCLLR QGRIYYPRKS GFTAAMIHCA GSAWFGKRKN RIKRCLAFMK 490 500 510 520 530 540 PLIALRKQEN DVLTYGALEW KLVDDQNDFV SFSRTHEGKE LIYFFHQGRE VRRVRLRDLK 550 560 570 IASDKRIYDA WTEEALHDDD VVDIQPGDFS FLGRSKFC

References

[1]	"Catalytic properties of the cloned amylase from Bacillus licheniformis." Kim I.C., Cha J.H., Kim J.R., Jang S.Y., Seo B.C., Cheong T.K., Lee D.S., Choi Y.D., Park K.H. J. Biol. Chem. 267:22108-22114(1992) [PubMed: 1385394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27811 / CCRC 10494 / FERM P-1038.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67133 Genomic DNA. Translation: CAA47612.1.
PIR	A44326. S25010.
3D structure databases
ProteinModelPortal	Q04977.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004185. Glyco_hydro_13_lg-like_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02903. Alpha-amylase_N. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMYM_BACLI

Accession

Primary (citable) accession number: Q04977

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program