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Protein

GTPase NRas

Gene

Nras

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. actin cytoskeleton organization Source: Ensembl
  2. epithelial tube branching involved in lung morphogenesis Source: Ensembl
  3. GTP catabolic process Source: InterPro
  4. negative regulation of cell differentiation Source: Ensembl
  5. negative regulation of neuron apoptotic process Source: Ensembl
  6. negative regulation of skeletal muscle tissue development Source: Ensembl
  7. positive regulation of cell proliferation Source: Ensembl
  8. positive regulation of Rac protein signal transduction Source: Ensembl
  9. regulation of long-term neuronal synaptic plasticity Source: Ensembl
  10. regulation of synaptic transmission, GABAergic Source: Ensembl
  11. small GTPase mediated signal transduction Source: InterPro
  12. striated muscle cell differentiation Source: Ensembl
  13. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_195025. Signaling by SCF-KIT.
REACT_195026. SHC1 events in ERBB4 signaling.
REACT_195029. SHC1 events in ERBB2 signaling.
REACT_195202. FCERI mediated MAPK activation.
REACT_195203. GRB2 events in ERBB2 signaling.
REACT_196848. EGFR Transactivation by Gastrin.
REACT_198665. Signaling by FGFR mutants.
REACT_199246. Activation of RAS in B cells.
REACT_199249. Signaling by constitutively active EGFR.
REACT_204559. SOS-mediated signalling.
REACT_206819. Downstream signal transduction.
REACT_212418. DAP12 signaling.
REACT_239061. Tie2 Signaling.
REACT_240421. FRS2-mediated cascade.
REACT_243035. GRB2 events in EGFR signaling.
REACT_247187. VEGFR2 mediated cell proliferation.
REACT_251180. NCAM signaling for neurite out-growth.
REACT_252691. SHC-mediated signalling.
REACT_254614. SHC1 events in EGFR signaling.
REACT_254788. RAF activation.
REACT_261049. p38MAPK events.
REACT_262476. Signalling to RAS.
REACT_79133. SHC-mediated cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase NRas
Alternative name(s):
Transforming protein N-Ras
Gene namesi
Name:Nras
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi3205. Nras.

Subcellular locationi

Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Golgi apparatus membrane By similarity; Lipid-anchor By similarity
Note: Shuttles between the plasma membrane and the Golgi apparatus.By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi membrane Source: UniProtKB-SubCell
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186GTPase NRasPRO_0000043014Add
BLAST
Propeptidei187 – 1893Removed in mature formBy similarityPRO_0000043015

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi181 – 1811S-palmitoyl cysteineBy similarity
Modified residuei186 – 1861Cysteine methyl esterBy similarity
Lipidationi186 – 1861S-farnesyl cysteineBy similarity

Post-translational modificationi

Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (By similarity).By similarity
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiQ04970.
PRIDEiQ04970.

PTM databases

PhosphoSiteiQ04970.

Expressioni

Gene expression databases

GenevestigatoriQ04970.

Interactioni

Subunit structurei

Interacts (active GTP-bound form) with RASSF7 (By similarity). Interacts (active GTP-bound form preferentially) with RGS14.By similarity1 Publication

Protein-protein interaction databases

BioGridi246744. 1 interaction.
IntActiQ04970. 2 interactions.
MINTiMINT-4789999.
STRINGi10116.ENSRNOP00000036381.

Structurei

3D structure databases

ProteinModelPortaliQ04970.
SMRiQ04970. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 18520Hypervariable regionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ04970.
KOiK07828.
OMAiRSSYDEI.
OrthoDBiEOG7QVM41.
PhylomeDBiQ04970.
TreeFamiTF312796.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04970-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI
110 120 130 140 150
KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ
160 170 180
GVEDAFYTLV REIRQYRMKK LNSSEDGTQG CMGLPCVVM
Length:189
Mass (Da):21,243
Last modified:February 1, 1994 - v1
Checksum:i6898D3F6815B1F82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68394 Genomic DNA. Translation: CAA48460.1.
BC098659 mRNA. Translation: AAH98659.1.
PIRiI83212. S26621.
RefSeqiNP_542944.1. NM_080766.2.
XP_006233121.1. XM_006233059.2.
UniGeneiRn.217722.

Genome annotation databases

EnsembliENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
GeneIDi24605.
KEGGirno:24605.
UCSCiRGD:3205. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68394 Genomic DNA. Translation: CAA48460.1.
BC098659 mRNA. Translation: AAH98659.1.
PIRiI83212. S26621.
RefSeqiNP_542944.1. NM_080766.2.
XP_006233121.1. XM_006233059.2.
UniGeneiRn.217722.

3D structure databases

ProteinModelPortaliQ04970.
SMRiQ04970. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246744. 1 interaction.
IntActiQ04970. 2 interactions.
MINTiMINT-4789999.
STRINGi10116.ENSRNOP00000036381.

PTM databases

PhosphoSiteiQ04970.

Proteomic databases

PaxDbiQ04970.
PRIDEiQ04970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
GeneIDi24605.
KEGGirno:24605.
UCSCiRGD:3205. rat.

Organism-specific databases

CTDi4893.
RGDi3205. Nras.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ04970.
KOiK07828.
OMAiRSSYDEI.
OrthoDBiEOG7QVM41.
PhylomeDBiQ04970.
TreeFamiTF312796.

Enzyme and pathway databases

ReactomeiREACT_195025. Signaling by SCF-KIT.
REACT_195026. SHC1 events in ERBB4 signaling.
REACT_195029. SHC1 events in ERBB2 signaling.
REACT_195202. FCERI mediated MAPK activation.
REACT_195203. GRB2 events in ERBB2 signaling.
REACT_196848. EGFR Transactivation by Gastrin.
REACT_198665. Signaling by FGFR mutants.
REACT_199246. Activation of RAS in B cells.
REACT_199249. Signaling by constitutively active EGFR.
REACT_204559. SOS-mediated signalling.
REACT_206819. Downstream signal transduction.
REACT_212418. DAP12 signaling.
REACT_239061. Tie2 Signaling.
REACT_240421. FRS2-mediated cascade.
REACT_243035. GRB2 events in EGFR signaling.
REACT_247187. VEGFR2 mediated cell proliferation.
REACT_251180. NCAM signaling for neurite out-growth.
REACT_252691. SHC-mediated signalling.
REACT_254614. SHC1 events in EGFR signaling.
REACT_254788. RAF activation.
REACT_261049. p38MAPK events.
REACT_262476. Signalling to RAS.
REACT_79133. SHC-mediated cascade.

Miscellaneous databases

NextBioi603824.
PROiQ04970.

Gene expression databases

GenevestigatoriQ04970.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway."
    Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.
    Gene 130:155-156(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The rat N-ras gene; interference of pseudogenes with the detection of activating point mutations."
    van Kranen H.J., van Steeg H., Schoren L., Faessen P., de Vries A., van Iersel P.W., van Kreijl C.F.
    Carcinogenesis 15:307-311(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  4. "Possible involvement of c-myc but not ras genes in hepatocellular carcinomas developing after spontaneous hepatitis in LEC rats."
    Fujimoto Y., Ishizaka Y., Tahira T., Sone H., Takahashi H., Enomoto K., Mori M., Sugimura T., Nagao M.
    Mol. Carcinog. 4:269-274(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-96.
  5. "Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors."
    McMahon G., Davis E.F., Huber L.J., Kim Y., Wogan G.N.
    Proc. Natl. Acad. Sci. U.S.A. 87:1104-1108(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 8-22.
  6. Cited for: INTERACTION WITH RGS14.

Entry informationi

Entry nameiRASN_RAT
AccessioniPrimary (citable) accession number: Q04970
Secondary accession number(s): Q4KMB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 4, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.