Q04970 (RASN_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase NRas

Alternative name(s):
Transforming protein N-Ras

Gene names

Name:

Nras

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Enzyme regulation	Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Subunit structure	Interacts (active GTP-bound form preferentially) with RGS14. Ref.6
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Note: Shuttles between the plasma membrane and the Golgi apparatus By similarity.
Post-translational modification	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Membrane
Disease	Proto-oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Palmitate Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: Reactome
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	GTPase NRas		PRO_0000043014
	Propeptide	187 – 189	3	Removed in mature form By similarity		PRO_0000043015
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	116 – 119	4	GTP By similarity
	Region	166 – 185	20	Hypervariable region By similarity
	Motif	32 – 40	9	Effector region By similarity
Amino acid modifications
	Modified residue	2	1	Phosphothreonine By similarity
	Modified residue	4	1	Phosphotyrosine By similarity
	Modified residue	186	1	Cysteine methyl ester By similarity
	Lipidation	181	1	S-palmitoyl cysteine By similarity
	Lipidation	186	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04970 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 6898D3F6815B1F82	FASTA	189	21,243
	10 20 30 40 50 60 MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG 70 80 90 100 110 120 QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL 130 140 150 160 170 180 PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSEDGTQG CMGLPCVVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway." Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E. Gene 130:155-156(1993) [PubMed: 8344525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The rat N-ras gene; interference of pseudogenes with the detection of activating point mutations." van Kranen H.J., van Steeg H., Schoren L., Faessen P., de Vries A., van Iersel P.W., van Kreijl C.F. Carcinogenesis 15:307-311(1994) [PubMed: 8313523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[4]	"Possible involvement of c-myc but not ras genes in hepatocellular carcinomas developing after spontaneous hepatitis in LEC rats." Fujimoto Y., Ishizaka Y., Tahira T., Sone H., Takahashi H., Enomoto K., Mori M., Sugimura T., Nagao M. Mol. Carcinog. 4:269-274(1991) [PubMed: 1714740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-96.
[5]	"Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors." McMahon G., Davis E.F., Huber L.J., Kim Y., Wogan G.N. Proc. Natl. Acad. Sci. U.S.A. 87:1104-1108(1990) [PubMed: 2105496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 8-22.
[6]	"Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector." Willard F.S., Willard M.D., Kimple A.J., Soundararajan M., Oestreich E.A., Li X., Sowa N.A., Kimple R.J., Doyle D.A., Der C.J., Zylka M.J., Snider W.D., Siderovski D.P. PLoS ONE 4:E4884-E4884(2009) [PubMed: 19319189] [Abstract] Cited for: INTERACTION WITH RGS14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68394 Genomic DNA. Translation: CAA48460.1. BC098659 mRNA. Translation: AAH98659.1.
IPI	IPI00190869.
PIR	S26621. I83212.
RefSeq	NP_542944.1. NM_080766.2.
UniGene	Rn.217722.
3D structure databases
ProteinModelPortal	Q04970.
SMR	Q04970. Positions 1-166.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4789999.
STRING	Q04970.
PTM databases
PhosphoSite	Q04970.
Proteomic databases
PRIDE	Q04970.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
GeneID	24605.
KEGG	rno:24605.
UCSC	BC098659. rat.
Organism-specific databases
CTD	4893.
RGD	3205. Nras.
Phylogenomic databases
eggNOG	maNOG17728.
GeneTree	ENSGT00560000076901.
HOVERGEN	HBG009351.
InParanoid	Q04970.
OMA	DIGTYRE.
OrthoDB	EOG4BRWMX.
PhylomeDB	Q04970.
Enzyme and pathway databases
Reactome	REACT_111984. Signal Transduction.
Gene expression databases
ArrayExpress	Q04970.
Genevestigator	Q04970.
GermOnline	ENSRNOG00000023079. Rattus norvegicus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
KO	K07828.
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603824.

Entry information

Entry name

RASN_RAT

Accession

Primary (citable) accession number: Q04970
Secondary accession number(s): Q4KMB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program