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Protein

GTPase NRas

Gene

Nras

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-112412. SOS-mediated signalling.
R-RNO-1169092. Activation of RAS in B cells.
R-RNO-1250347. SHC1 events in ERBB4 signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-167044. Signalling to RAS.
R-RNO-171007. p38MAPK events.
R-RNO-179812. GRB2 events in EGFR signaling.
R-RNO-180336. SHC1 events in EGFR signaling.
R-RNO-186763. Downstream signal transduction.
R-RNO-1963640. GRB2 events in ERBB2 signaling.
R-RNO-210993. Tie2 Signaling.
R-RNO-2179392. EGFR Transactivation by Gastrin.
R-RNO-2424491. DAP12 signaling.
R-RNO-2428933. SHC-related events triggered by IGF1R.
R-RNO-2871796. FCERI mediated MAPK activation.
R-RNO-375165. NCAM signaling for neurite out-growth.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5621575. CD209 (DC-SIGN) signaling.
R-RNO-5654688. SHC-mediated cascade:FGFR1.
R-RNO-5654693. FRS-mediated FGFR1 signaling.
R-RNO-5654699. SHC-mediated cascade:FGFR2.
R-RNO-5654700. FRS-mediated FGFR2 signaling.
R-RNO-5654704. SHC-mediated cascade:FGFR3.
R-RNO-5654706. FRS-mediated FGFR3 signaling.
R-RNO-5654712. FRS-mediated FGFR4 signaling.
R-RNO-5654719. SHC-mediated cascade:FGFR4.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5673000. RAF activation.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5675221. Negative regulation of MAPK pathway.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-74751. Insulin receptor signalling cascade.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-RNO-8851805. MET activates RAS signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase NRas
Alternative name(s):
Transforming protein N-Ras
Gene namesi
Name:Nras
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3205. Nras.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000430141 – 186GTPase NRasAdd BLAST186
PropeptideiPRO_0000043015187 – 189Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi181S-palmitoyl cysteineBy similarity1
Modified residuei186Cysteine methyl esterBy similarity1
Lipidationi186S-farnesyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (By similarity).By similarity
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiQ04970.
PRIDEiQ04970.

PTM databases

PhosphoSitePlusiQ04970.

Expressioni

Gene expression databases

BgeeiENSRNOG00000023079.

Interactioni

Subunit structurei

Interacts (active GTP-bound form) with RASSF7 (By similarity). Interacts (active GTP-bound form preferentially) with RGS14.By similarity1 Publication

Protein-protein interaction databases

BioGridi246744. 1 interactor.
IntActiQ04970. 2 interactors.
MINTiMINT-4789999.
STRINGi10116.ENSRNOP00000036381.

Structurei

3D structure databases

ProteinModelPortaliQ04970.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 185Hypervariable regionBy similarityAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ04970.
KOiK07828.
OMAiRSSYDEI.
OrthoDBiEOG091G0UAU.
PhylomeDBiQ04970.
TreeFamiTF312796.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI
110 120 130 140 150
KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ
160 170 180
GVEDAFYTLV REIRQYRMKK LNSSEDGTQG CMGLPCVVM
Length:189
Mass (Da):21,243
Last modified:February 1, 1994 - v1
Checksum:i6898D3F6815B1F82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68394 Genomic DNA. Translation: CAA48460.1.
BC098659 mRNA. Translation: AAH98659.1.
PIRiI83212. S26621.
RefSeqiNP_542944.1. NM_080766.2.
XP_006233121.1. XM_006233059.2.
UniGeneiRn.217722.

Genome annotation databases

EnsembliENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
GeneIDi24605.
KEGGirno:24605.
UCSCiRGD:3205. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68394 Genomic DNA. Translation: CAA48460.1.
BC098659 mRNA. Translation: AAH98659.1.
PIRiI83212. S26621.
RefSeqiNP_542944.1. NM_080766.2.
XP_006233121.1. XM_006233059.2.
UniGeneiRn.217722.

3D structure databases

ProteinModelPortaliQ04970.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246744. 1 interactor.
IntActiQ04970. 2 interactors.
MINTiMINT-4789999.
STRINGi10116.ENSRNOP00000036381.

PTM databases

PhosphoSitePlusiQ04970.

Proteomic databases

PaxDbiQ04970.
PRIDEiQ04970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
GeneIDi24605.
KEGGirno:24605.
UCSCiRGD:3205. rat.

Organism-specific databases

CTDi4893.
RGDi3205. Nras.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ04970.
KOiK07828.
OMAiRSSYDEI.
OrthoDBiEOG091G0UAU.
PhylomeDBiQ04970.
TreeFamiTF312796.

Enzyme and pathway databases

ReactomeiR-RNO-112412. SOS-mediated signalling.
R-RNO-1169092. Activation of RAS in B cells.
R-RNO-1250347. SHC1 events in ERBB4 signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-167044. Signalling to RAS.
R-RNO-171007. p38MAPK events.
R-RNO-179812. GRB2 events in EGFR signaling.
R-RNO-180336. SHC1 events in EGFR signaling.
R-RNO-186763. Downstream signal transduction.
R-RNO-1963640. GRB2 events in ERBB2 signaling.
R-RNO-210993. Tie2 Signaling.
R-RNO-2179392. EGFR Transactivation by Gastrin.
R-RNO-2424491. DAP12 signaling.
R-RNO-2428933. SHC-related events triggered by IGF1R.
R-RNO-2871796. FCERI mediated MAPK activation.
R-RNO-375165. NCAM signaling for neurite out-growth.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5621575. CD209 (DC-SIGN) signaling.
R-RNO-5654688. SHC-mediated cascade:FGFR1.
R-RNO-5654693. FRS-mediated FGFR1 signaling.
R-RNO-5654699. SHC-mediated cascade:FGFR2.
R-RNO-5654700. FRS-mediated FGFR2 signaling.
R-RNO-5654704. SHC-mediated cascade:FGFR3.
R-RNO-5654706. FRS-mediated FGFR3 signaling.
R-RNO-5654712. FRS-mediated FGFR4 signaling.
R-RNO-5654719. SHC-mediated cascade:FGFR4.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5673000. RAF activation.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5675221. Negative regulation of MAPK pathway.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-74751. Insulin receptor signalling cascade.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-RNO-8851805. MET activates RAS signaling.

Miscellaneous databases

PROiQ04970.

Gene expression databases

BgeeiENSRNOG00000023079.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASN_RAT
AccessioniPrimary (citable) accession number: Q04970
Secondary accession number(s): Q4KMB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.