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Q04970

- RASN_RAT

UniProt

Q04970 - RASN_RAT

Protein

GTPase NRas

Gene

Nras

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTPBy similarity
    Nucleotide bindingi57 – 615GTPBy similarity
    Nucleotide bindingi116 – 1194GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. actin cytoskeleton organization Source: Ensembl
    2. GTP catabolic process Source: InterPro
    3. negative regulation of neuron apoptotic process Source: Ensembl
    4. positive regulation of cell proliferation Source: Ensembl
    5. positive regulation of Rac protein signal transduction Source: Ensembl
    6. regulation of long-term neuronal synaptic plasticity Source: Ensembl
    7. regulation of synaptic transmission, GABAergic Source: Ensembl
    8. small GTPase mediated signal transduction Source: InterPro
    9. striated muscle cell differentiation Source: Ensembl
    10. visual learning Source: Ensembl

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_195025. Signaling by SCF-KIT.
    REACT_195026. SHC1 events in ERBB4 signaling.
    REACT_195029. SHC1 events in ERBB2 signaling.
    REACT_195202. FCERI mediated MAPK activation.
    REACT_195203. GRB2 events in ERBB2 signaling.
    REACT_196848. EGFR Transactivation by Gastrin.
    REACT_198665. Signaling by FGFR mutants.
    REACT_199246. Activation of RAS in B cells.
    REACT_199249. Signaling by constitutively active EGFR.
    REACT_204559. SOS-mediated signalling.
    REACT_206819. Downstream signal transduction.
    REACT_212418. DAP12 signaling.
    REACT_79133. SHC-mediated cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase NRas
    Alternative name(s):
    Transforming protein N-Ras
    Gene namesi
    Name:Nras
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi3205. Nras.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Golgi apparatus membrane By similarity; Lipid-anchor By similarity
    Note: Shuttles between the plasma membrane and the Golgi apparatus.By similarity

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Golgi apparatus, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 186186GTPase NRasPRO_0000043014Add
    BLAST
    Propeptidei187 – 1893Removed in mature formBy similarityPRO_0000043015

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi181 – 1811S-palmitoyl cysteineBy similarity
    Modified residuei186 – 1861Cysteine methyl esterBy similarity
    Lipidationi186 – 1861S-farnesyl cysteineBy similarity

    Post-translational modificationi

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity.By similarity
    Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    PaxDbiQ04970.
    PRIDEiQ04970.

    PTM databases

    PhosphoSiteiQ04970.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04970.

    Interactioni

    Subunit structurei

    Interacts (active GTP-bound form) with RASSF7 By similarity. Interacts (active GTP-bound form preferentially) with RGS14.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi246744. 1 interaction.
    IntActiQ04970. 2 interactions.
    MINTiMINT-4789999.
    STRINGi10116.ENSRNOP00000036381.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04970.
    SMRiQ04970. Positions 1-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 18520Hypervariable regionBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector region

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ras family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000117221.
    HOGENOMiHOG000233973.
    HOVERGENiHBG009351.
    InParanoidiQ04970.
    KOiK07828.
    OMAiSNEDGNQ.
    OrthoDBiEOG7QVM41.
    PhylomeDBiQ04970.
    TreeFamiTF312796.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04970-1 [UniParc]FASTAAdd to Basket

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    MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET    50
    CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI 100
    KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ 150
    GVEDAFYTLV REIRQYRMKK LNSSEDGTQG CMGLPCVVM 189
    Length:189
    Mass (Da):21,243
    Last modified:February 1, 1994 - v1
    Checksum:i6898D3F6815B1F82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68394 Genomic DNA. Translation: CAA48460.1.
    BC098659 mRNA. Translation: AAH98659.1.
    PIRiI83212. S26621.
    RefSeqiNP_542944.1. NM_080766.2.
    XP_006233121.1. XM_006233059.1.
    UniGeneiRn.217722.

    Genome annotation databases

    EnsembliENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
    GeneIDi24605.
    KEGGirno:24605.
    UCSCiRGD:3205. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68394 Genomic DNA. Translation: CAA48460.1 .
    BC098659 mRNA. Translation: AAH98659.1 .
    PIRi I83212. S26621.
    RefSeqi NP_542944.1. NM_080766.2.
    XP_006233121.1. XM_006233059.1.
    UniGenei Rn.217722.

    3D structure databases

    ProteinModelPortali Q04970.
    SMRi Q04970. Positions 1-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246744. 1 interaction.
    IntActi Q04970. 2 interactions.
    MINTi MINT-4789999.
    STRINGi 10116.ENSRNOP00000036381.

    PTM databases

    PhosphoSitei Q04970.

    Proteomic databases

    PaxDbi Q04970.
    PRIDEi Q04970.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000039572 ; ENSRNOP00000036381 ; ENSRNOG00000023079 .
    GeneIDi 24605.
    KEGGi rno:24605.
    UCSCi RGD:3205. rat.

    Organism-specific databases

    CTDi 4893.
    RGDi 3205. Nras.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000117221.
    HOGENOMi HOG000233973.
    HOVERGENi HBG009351.
    InParanoidi Q04970.
    KOi K07828.
    OMAi SNEDGNQ.
    OrthoDBi EOG7QVM41.
    PhylomeDBi Q04970.
    TreeFami TF312796.

    Enzyme and pathway databases

    Reactomei REACT_195025. Signaling by SCF-KIT.
    REACT_195026. SHC1 events in ERBB4 signaling.
    REACT_195029. SHC1 events in ERBB2 signaling.
    REACT_195202. FCERI mediated MAPK activation.
    REACT_195203. GRB2 events in ERBB2 signaling.
    REACT_196848. EGFR Transactivation by Gastrin.
    REACT_198665. Signaling by FGFR mutants.
    REACT_199246. Activation of RAS in B cells.
    REACT_199249. Signaling by constitutively active EGFR.
    REACT_204559. SOS-mediated signalling.
    REACT_206819. Downstream signal transduction.
    REACT_212418. DAP12 signaling.
    REACT_79133. SHC-mediated cascade.

    Miscellaneous databases

    NextBioi 603824.
    PROi Q04970.

    Gene expression databases

    Genevestigatori Q04970.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway."
      Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.
      Gene 130:155-156(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "The rat N-ras gene; interference of pseudogenes with the detection of activating point mutations."
      van Kranen H.J., van Steeg H., Schoren L., Faessen P., de Vries A., van Iersel P.W., van Kreijl C.F.
      Carcinogenesis 15:307-311(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spleen.
    4. "Possible involvement of c-myc but not ras genes in hepatocellular carcinomas developing after spontaneous hepatitis in LEC rats."
      Fujimoto Y., Ishizaka Y., Tahira T., Sone H., Takahashi H., Enomoto K., Mori M., Sugimura T., Nagao M.
      Mol. Carcinog. 4:269-274(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-96.
    5. "Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors."
      McMahon G., Davis E.F., Huber L.J., Kim Y., Wogan G.N.
      Proc. Natl. Acad. Sci. U.S.A. 87:1104-1108(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 8-22.
    6. Cited for: INTERACTION WITH RGS14.

    Entry informationi

    Entry nameiRASN_RAT
    AccessioniPrimary (citable) accession number: Q04970
    Secondary accession number(s): Q4KMB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3