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Q04970 (RASN_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase NRas
Alternative name(s):
Transforming protein N-Ras
Gene names
Name:Nras
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Subunit structure

Interacts (active GTP-bound form) with RASSF7 By similarity. Interacts (active GTP-bound form preferentially) with RGS14. Ref.6

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Note: Shuttles between the plasma membrane and the Golgi apparatus By similarity.

Post-translational modification

Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity.

Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs) By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
   Cellular componentCell membrane
Golgi apparatus
Membrane
   DiseaseProto-oncogene
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Lipoprotein
Methylation
Palmitate
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from electronic annotation. Source: InterPro

actin cytoskeleton organization

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of Rac protein signal transduction

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

regulation of long-term neuronal synaptic plasticity

Inferred from electronic annotation. Source: Ensembl

regulation of synaptic transmission, GABAergic

Inferred from electronic annotation. Source: Ensembl

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

striated muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

visual learning

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.6. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 186186GTPase NRas
PRO_0000043014
Propeptide187 – 1893Removed in mature form By similarity
PRO_0000043015

Regions

Nucleotide binding10 – 178GTP By similarity
Nucleotide binding57 – 615GTP By similarity
Nucleotide binding116 – 1194GTP By similarity
Region166 – 18520Hypervariable region By similarity
Motif32 – 409Effector region

Amino acid modifications

Modified residue1861Cysteine methyl ester By similarity
Lipidation1811S-palmitoyl cysteine By similarity
Lipidation1861S-farnesyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04970 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 6898D3F6815B1F82

FASTA18921,243
        10         20         30         40         50         60 
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG 

        70         80         90        100        110        120 
QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL 

       130        140        150        160        170        180 
PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSEDGTQG 


CMGLPCVVM 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway."
Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.
Gene 130:155-156(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"The rat N-ras gene; interference of pseudogenes with the detection of activating point mutations."
van Kranen H.J., van Steeg H., Schoren L., Faessen P., de Vries A., van Iersel P.W., van Kreijl C.F.
Carcinogenesis 15:307-311(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[4]"Possible involvement of c-myc but not ras genes in hepatocellular carcinomas developing after spontaneous hepatitis in LEC rats."
Fujimoto Y., Ishizaka Y., Tahira T., Sone H., Takahashi H., Enomoto K., Mori M., Sugimura T., Nagao M.
Mol. Carcinog. 4:269-274(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-96.
[5]"Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors."
McMahon G., Davis E.F., Huber L.J., Kim Y., Wogan G.N.
Proc. Natl. Acad. Sci. U.S.A. 87:1104-1108(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 8-22.
[6]"Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector."
Willard F.S., Willard M.D., Kimple A.J., Soundararajan M., Oestreich E.A., Li X., Sowa N.A., Kimple R.J., Doyle D.A., Der C.J., Zylka M.J., Snider W.D., Siderovski D.P.
PLoS ONE 4:E4884-E4884(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGS14.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68394 Genomic DNA. Translation: CAA48460.1.
BC098659 mRNA. Translation: AAH98659.1.
PIRS26621. I83212.
RefSeqNP_542944.1. NM_080766.2.
XP_006233121.1. XM_006233059.1.
UniGeneRn.217722.

3D structure databases

ProteinModelPortalQ04970.
SMRQ04970. Positions 1-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246744. 1 interaction.
IntActQ04970. 2 interactions.
MINTMINT-4789999.
STRING10116.ENSRNOP00000036381.

PTM databases

PhosphoSiteQ04970.

Proteomic databases

PaxDbQ04970.
PRIDEQ04970.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000039572; ENSRNOP00000036381; ENSRNOG00000023079.
GeneID24605.
KEGGrno:24605.
UCSCRGD:3205. rat.

Organism-specific databases

CTD4893.
RGD3205. Nras.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00750000117221.
HOGENOMHOG000233973.
HOVERGENHBG009351.
InParanoidQ04970.
KOK07828.
OMASNEDGNQ.
OrthoDBEOG7QVM41.
PhylomeDBQ04970.
TreeFamTF312796.

Enzyme and pathway databases

ReactomeREACT_212996. Signal Transduction.

Gene expression databases

GenevestigatorQ04970.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603824.
PROQ04970.

Entry information

Entry nameRASN_RAT
AccessionPrimary (citable) accession number: Q04970
Secondary accession number(s): Q4KMB1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families