Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q04962

- FA12_CAVPO

UniProt

Q04962 - FA12_CAVPO

Protein

Coagulation factor XII

Gene

F12

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa By similarity.By similarity

    Catalytic activityi

    Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei398 – 3981Charge relay systemBy similarity
    Active sitei447 – 4471Charge relay systemBy similarity
    Active sitei551 – 5511Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. blood coagulation Source: UniProtKB-KW
    2. fibrinolysis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Blood coagulation, Fibrinolysis, Hemostasis

    Protein family/group databases

    MEROPSiS01.211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coagulation factor XII (EC:3.4.21.38)
    Alternative name(s):
    Hageman factor
    Short name:
    HAF
    Cleaved into the following 2 chains:
    Gene namesi
    Name:F12
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: InterPro

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 18›181 PublicationAdd
    BLAST
    Chaini19 – 358340Coagulation factor XIIa heavy chainPRO_0000027831Add
    BLAST
    Chaini359 – 603245Coagulation factor XIIa light chainPRO_0000027832Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi46 ↔ 72By similarity
    Disulfide bondi60 ↔ 87By similarity
    Disulfide bondi97 ↔ 109By similarity
    Disulfide bondi103 ↔ 118By similarity
    Disulfide bondi120 ↔ 129By similarity
    Disulfide bondi134 ↔ 162By similarity
    Disulfide bondi160 ↔ 169By similarity
    Disulfide bondi177 ↔ 188By similarity
    Disulfide bondi182 ↔ 197By similarity
    Disulfide bondi199 ↔ 208By similarity
    Disulfide bondi216 ↔ 294By similarity
    Disulfide bondi237 ↔ 276By similarity
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi265 ↔ 289By similarity
    Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi345 ↔ 472By similarity
    Disulfide bondi383 ↔ 399By similarity
    Disulfide bondi391 ↔ 461By similarity
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi422 ↔ 425By similarity
    Disulfide bondi488 ↔ 557By similarity
    Disulfide bondi520 ↔ 536By similarity
    Disulfide bondi547 ↔ 578By similarity

    Post-translational modificationi

    O- and N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Interactioni

    Subunit structurei

    Interacts with HRG; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding, inhibits factor XII autoactivation and contact-initiated coagulation.By similarity

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000018858.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04962.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 8949Fibronectin type-IIPROSITE-ProRule annotationAdd
    BLAST
    Domaini93 – 13038EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini132 – 17241Fibronectin type-IPROSITE-ProRule annotationAdd
    BLAST
    Domaini173 – 20937EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini216 – 29479KringlePROSITE-ProRule annotationAdd
    BLAST
    Domaini359 – 602244Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi312 – 34231Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 fibronectin type-I domain.PROSITE-ProRule annotation
    Contains 1 fibronectin type-II domain.PROSITE-ProRule annotation
    Contains 1 kringle domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Kringle, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000237314.
    HOVERGENiHBG004345.
    InParanoidiQ04962.

    Family and domain databases

    Gene3Di2.10.10.10. 1 hit.
    2.40.20.10. 1 hit.
    InterProiIPR014394. Coagulation_fac_XIIa/HGFA.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000083. Fibronectin_type1.
    IPR000562. FN_type2_col-bd.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00008. EGF. 2 hits.
    PF00039. fn1. 1 hit.
    PF00040. fn2. 1 hit.
    PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001146. Factor_XII_HGFA. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00181. EGF. 2 hits.
    SM00058. FN1. 1 hit.
    SM00059. FN2. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 2 hits.
    PROSITEiPS00022. EGF_1. 2 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01253. FN1_1. 1 hit.
    PS51091. FN1_2. 1 hit.
    PS00023. FN2_1. 1 hit.
    PS51092. FN2_2. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04962-1 [UniParc]FASTAAdd to Basket

    « Hide

    GRLLLGSLLV SLESALSAPP PWKAPKERRH RAEEFTVGLT VTGEPCYFPF    50
    QYNRQLYHHC IHKGRPGPRP WCATTPNFDQ DQQWAYCLEP KKVKDHCSKH 100
    NPCQRGGICV NTLSSPHCLC PDHLTGKHCQ REKCFEPQLH RFFHENEIWF 150
    RTGPAGVAKC HCKGPDAHCK QMHSQECQTN PCLNGGRCLE VEGHHLCDCP 200
    MGYTGPFCDL DTTASCYEGR GVSYRGMART TVSGAKCQRW ASEATYRNMT 250
    AEQALRRGLG HHTFCRNPDN DTRPWCFVWM GNRLSWEYCD LAQCQYPPQP 300
    TATPHDRFEH PKLPSSRLSI LQTPQPTTQN QALANELPET SSLLCGQRLR 350
    KRLSSLSRIV GGLVALPGAH PYIAALYWGS NFCSGSLIAP CWVLTAAHCL 400
    QNRPAPEELK VVLGQDRHNQ SCEHCQTLAV HSYRLHEAFS PSSYLNDLAL 450
    LRLQKSADGS CAQLSPYVQT VCLPSGPAPP SESETTCCEV AGWGHQFEGA 500
    EEYSSFLQEA QVPLISSERC SSPEVHGDAF LSGMLCAGFL EGGTDACQGD 550
    SGGPLVCEDE AAEHRLILRG IVSWGSGCGD RNKPGVYTDV ASYLTWIQKH 600
    TAS 603
    Length:603
    Mass (Da):66,795
    Last modified:February 1, 1996 - v1
    Checksum:i48DC6B946FB9ED59
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68615 mRNA. Translation: CAA48600.1.
    PIRiS28941.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68615 mRNA. Translation: CAA48600.1 .
    PIRi S28941.

    3D structure databases

    ProteinModelPortali Q04962.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000018858.

    Protein family/group databases

    MEROPSi S01.211.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000237314.
    HOVERGENi HBG004345.
    InParanoidi Q04962.

    Family and domain databases

    Gene3Di 2.10.10.10. 1 hit.
    2.40.20.10. 1 hit.
    InterProi IPR014394. Coagulation_fac_XIIa/HGFA.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000083. Fibronectin_type1.
    IPR000562. FN_type2_col-bd.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00008. EGF. 2 hits.
    PF00039. fn1. 1 hit.
    PF00040. fn2. 1 hit.
    PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001146. Factor_XII_HGFA. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00181. EGF. 2 hits.
    SM00058. FN1. 1 hit.
    SM00059. FN2. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 2 hits.
    PROSITEi PS00022. EGF_1. 2 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01253. FN1_1. 1 hit.
    PS51091. FN1_2. 1 hit.
    PS00023. FN2_1. 1 hit.
    PS51092. FN2_2. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of guinea-pig Hageman factor: sequence around the cleavage site differs from the human molecule."
      Semba U., Yamamoto T., Kunisada T., Shibuya Y., Tanase S., Kambara T., Okabe H.
      Biochim. Biophys. Acta 1159:113-121(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-37; 318-332 AND 359-373.
      Tissue: Liver.

    Entry informationi

    Entry nameiFA12_CAVPO
    AccessioniPrimary (citable) accession number: Q04962
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3