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Q04958

- NTE1_YEAST

UniProt

Q04958 - NTE1_YEAST

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Protein

Lysophospholipase NTE1

Gene

NTE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium.3 Publications

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Positively regulated by SEC14. Inhibited by organophosphorus esters in the order phenyl saligenin phosphate (PSP) > phenyldipentyl phosphinate (PDPP) = diisopropyl fluorophosphate (DFP) > and paraoxon (PXN).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1406 – 14061

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi803 – 947145cNMP 1Add
BLAST
Nucleotide bindingi943 – 1074132cNMP 2Add
BLAST

GO - Molecular functioni

  1. lysophospholipase activity Source: SGD

GO - Biological processi

  1. phosphatidylcholine catabolic process Source: SGD
  2. regulation of phospholipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32654-MONOMER.
YEAST:G3O-32654-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase NTE1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:NTE1
Ordered Locus Names:YML059C
ORF Names:YM9958.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML059c.
SGDiS000004524. NTE1.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1406 – 14061S → A: Loss of esterase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16791679Lysophospholipase NTE1PRO_0000172528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei300 – 3001Phosphoserine1 Publication
Modified residuei312 – 3121Phosphoserine1 Publication
Modified residuei632 – 6321Phosphoserine1 Publication
Modified residuei634 – 6341Phosphoserine3 Publications
Modified residuei653 – 6531Phosphoserine1 Publication
Modified residuei661 – 6611Phosphoserine1 Publication
Modified residuei670 – 6701Phosphoserine1 Publication
Modified residuei680 – 6801Phosphoserine1 Publication
Modified residuei739 – 7391Phosphoserine1 Publication
Modified residuei803 – 8031Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04958.
PaxDbiQ04958.
PeptideAtlasiQ04958.

Expressioni

Gene expression databases

GenevestigatoriQ04958.

Interactioni

Protein-protein interaction databases

BioGridi35107. 24 interactions.
DIPiDIP-5949N.
IntActiQ04958. 16 interactions.
MINTiMINT-653305.
STRINGi4932.YML059C.

Structurei

3D structure databases

ProteinModelPortaliQ04958.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4949CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini71 – 10333LumenalSequence AnalysisAdd
BLAST
Topological domaini125 – 16791555CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei50 – 7021HelicalSequence AnalysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1373 – 1537165PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1404 – 14085GXSXG

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi259 – 29739Asp-richAdd
BLAST
Compositional biasi713 – 7186Poly-Ser

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 patatin domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0664.
GeneTreeiENSGT00390000002533.
HOGENOMiHOG000048680.
InParanoidiQ04958.
KOiK14676.
OMAiSPWTQTC.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04958-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRSMNCTTNN TNNTGQNTKN SLGSSFNSSN YTSYRFQTCL TDQIISEAQT
60 70 80 90 100
WSLSSLFNFS WVVSYFVMGA SRMIFRYGWY LATLSLLRIP KWIFFKLHHV
110 120 130 140 150
QFTLSFWLIL FALAVIVFVT YTIMKERILS QYKRLTPEFL PLENTGKSGS
160 170 180 190 200
SANINAASTQ SANAPPAIGS STTGASSIID SKKHSLKDGN ENETFLSSYL
210 220 230 240 250
DQFLSAIKIF GYLEKPVFHD LTKNMKTQKM DEGEILLLDS TIGFAIVVEG
260 270 280 290 300
TLQLYHEVDH SDKDHGDETD HSDTDGLDDQ DRDEEDEEED DDIDNYDTKS
310 320 330 340 350
CSSNLIDEED ESVGYIHLKN GLGNFQLLNT VKPGNPLTSL VSILNLFTHS
360 370 380 390 400
MSSYGNSNFP SELSSPIDTT VSVNNMFCSS EQNFSNTDSM TNSTNSFPTF
410 420 430 440 450
PSSMPKLVAR AATDCTIGII PPQSFAKLTA KYPRSASHII QMVLTKLYHV
460 470 480 490 500
TFQTAHDYLG LTKEIMDIEV LLNKSIVYEL PYYLKEAVIR KFKTVDKSSG
510 520 530 540 550
SADLEPKPKN SNASSKLKKP PKAKPSDGII QSLKIANANA NTSSNSLSLK
560 570 580 590 600
PEFTHHPSSR HVVLGSRDQF NPGDLLSNVP LSRTMDILSP NPIHNNNRNK
610 620 630 640 650
SNGINTSTSN QHKRSSRSSS NNASVHSKKF SSLSPELRNA QLSTSPLSLD
660 670 680 690 700
NTSVHDHIHP SPVHLKGRVS PRPNLLPTTS FSAAQEETED SALRMALVEA
710 720 730 740 750
MLTYLGVNKS NMSVSSSSIA NMSSLNSPQL NEMYSRRPSN ASFLMSPHCT
760 770 780 790 800
PSDISVASSF ASPQTQPTML RILPKEYTIS NKRHNKSKSQ DKKKPRAYKE
810 820 830 840 850
ELTPNLDFED VKKDFAQGIQ LKFFKKGTTI VEQNARGKGL FYIISGKVNV
860 870 880 890 900
TTNSSSSVVS SMSKPEQVSA QSSHKGENPH HTQHLLYSVG SGGIVGYLSS
910 920 930 940 950
LIGYKSFVNI VAKSDVYVGF LSSATLERLF DKYFLIYLRI SDSLTKLLSS
960 970 980 990 1000
RLLKLDHALE WVHLRASETL FSQGDSANGI YVVLNGRLRQ LQQQSLSNSN
1010 1020 1030 1040 1050
TSSEEVETQN IILGELAQGE SFGEVEVLTA MNRYSTIVAV RDSELARIPR
1060 1070 1080 1090 1100
TLFELLALEH PSIMIRVSRL VAKKIVGDRT VPALTGDPLS IKENDFTSLI
1110 1120 1130 1140 1150
PPTKASYSSS LSHKPQNITS GTITFRTITI LPITSGLPVE AFAMKLVQAF
1160 1170 1180 1190 1200
KQVGRTTIGL NQRTTLTHLG RHAFDRLSKL KQSGYFAELE EMYQTVVYIS
1210 1220 1230 1240 1250
DTPVKSNWTR TCIAQGDCIL LLADARSPSA EIGEYEKLLL NSKTTARTEL
1260 1270 1280 1290 1300
ILLHPERYVE PGLTHKWLRY RPWVHSHHHI QFSLTGTTLM NEGKMHVLNN
1310 1320 1330 1340 1350
GALALMDKLI QTEFSRKTQQ NISKLLPDSI KNTVENFSSR FMKSKRQYYT
1360 1370 1380 1390 1400
PVHRHKNDFL RLARILSGQA IGLVLGGGGA RGISHLGVIQ AIEEQGIPVD
1410 1420 1430 1440 1450
VIGGTSIGSF VGGLYAKDYD LVPIYGRVKK FAGRISSIWR MLTDLTWPVT
1460 1470 1480 1490 1500
SYTTGHEFNR GIWKTFGDTR IEDFWIQYYC NSTNITDSVQ EIHSFGYAWR
1510 1520 1530 1540 1550
YIRASMSLAG LLPPLEENGS MLLDGGYVDN LPVTEMRARG CQTIFAVDVG
1560 1570 1580 1590 1600
SADDRTPMEY GDSLNGFWII FNRWNPFSSH PNIPNMAEIQ VRLGYVASVN
1610 1620 1630 1640 1650
ALEKAKNTPG VVYVRPPIEE YATLDFSKFE EIYHVGVDYG RIFLQGLIDD
1660 1670
DKMPYIPGSQ ETTLNSQVPE FLLHRRNSI
Length:1,679
Mass (Da):187,133
Last modified:November 1, 1997 - v1
Checksum:i550FFCD4ACAF8E25
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46729 Genomic DNA. Translation: CAA86716.1.
BK006946 Genomic DNA. Translation: DAA09838.1.
PIRiS49802.
RefSeqiNP_013652.1. NM_001182418.1.

Genome annotation databases

EnsemblFungiiYML059C; YML059C; YML059C.
GeneIDi854943.
KEGGisce:YML059C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46729 Genomic DNA. Translation: CAA86716.1 .
BK006946 Genomic DNA. Translation: DAA09838.1 .
PIRi S49802.
RefSeqi NP_013652.1. NM_001182418.1.

3D structure databases

ProteinModelPortali Q04958.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35107. 24 interactions.
DIPi DIP-5949N.
IntActi Q04958. 16 interactions.
MINTi MINT-653305.
STRINGi 4932.YML059C.

Proteomic databases

MaxQBi Q04958.
PaxDbi Q04958.
PeptideAtlasi Q04958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML059C ; YML059C ; YML059C .
GeneIDi 854943.
KEGGi sce:YML059C.

Organism-specific databases

CYGDi YML059c.
SGDi S000004524. NTE1.

Phylogenomic databases

eggNOGi COG0664.
GeneTreei ENSGT00390000002533.
HOGENOMi HOG000048680.
InParanoidi Q04958.
KOi K14676.
OMAi SPWTQTC.
OrthoDBi EOG70W3NS.

Enzyme and pathway databases

BioCyci MetaCyc:G3O-32654-MONOMER.
YEAST:G3O-32654-MONOMER.

Miscellaneous databases

NextBioi 977998.
PROi Q04958.

Gene expression databases

Genevestigatori Q04958.

Family and domain databases

Gene3Di 2.60.120.10. 5 hits.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells."
    Zaccheo O., Dinsdale D., Meacock P.A., Glynn P.
    J. Biol. Chem. 279:24024-24033(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, MUTAGENESIS OF SER-1406.
  6. "Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p."
    Fernandez-Murray J.P., McMaster C.R.
    J. Biol. Chem. 280:8544-8552(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  7. "Inositol induces a profound alteration in the pattern and rate of synthesis and turnover of membrane lipids in Saccharomyces cerevisiae."
    Gaspar M.L., Aregullin M.A., Jesch S.A., Henry S.A.
    J. Biol. Chem. 281:22773-22785(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634 AND THR-803, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-312; SER-632; SER-634; SER-653; SER-661; SER-670; SER-680 AND SER-739, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNTE1_YEAST
AccessioniPrimary (citable) accession number: Q04958
Secondary accession number(s): D6VZB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3