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Q04958

- NTE1_YEAST

UniProt

Q04958 - NTE1_YEAST

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Protein

Lysophospholipase NTE1

Gene
NTE1, YML059C, YM9958.03C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium.3 Publications

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Positively regulated by SEC14. Inhibited by organophosphorus esters in the order phenyl saligenin phosphate (PSP) > phenyldipentyl phosphinate (PDPP) = diisopropyl fluorophosphate (DFP) > and paraoxon (PXN).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1406 – 14061

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi803 – 947145cNMP 1Add
BLAST
Nucleotide bindingi943 – 1074132cNMP 2Add
BLAST

GO - Molecular functioni

  1. lysophospholipase activity Source: SGD

GO - Biological processi

  1. phosphatidylcholine catabolic process Source: SGD
  2. regulation of phospholipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32654-MONOMER.
YEAST:G3O-32654-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase NTE1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:NTE1
Ordered Locus Names:YML059C
ORF Names:YM9958.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML059c.
SGDiS000004524. NTE1.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4949Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei50 – 7021Helical; Reviewed predictionAdd
BLAST
Topological domaini71 – 10333Lumenal Reviewed predictionAdd
BLAST
Transmembranei104 – 12421Helical; Reviewed predictionAdd
BLAST
Topological domaini125 – 16791555Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1406 – 14061S → A: Loss of esterase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16791679Lysophospholipase NTE1PRO_0000172528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei300 – 3001Phosphoserine1 Publication
Modified residuei312 – 3121Phosphoserine1 Publication
Modified residuei632 – 6321Phosphoserine1 Publication
Modified residuei634 – 6341Phosphoserine3 Publications
Modified residuei653 – 6531Phosphoserine1 Publication
Modified residuei661 – 6611Phosphoserine1 Publication
Modified residuei670 – 6701Phosphoserine1 Publication
Modified residuei680 – 6801Phosphoserine1 Publication
Modified residuei739 – 7391Phosphoserine1 Publication
Modified residuei803 – 8031Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04958.
PaxDbiQ04958.
PeptideAtlasiQ04958.

Expressioni

Gene expression databases

GenevestigatoriQ04958.

Interactioni

Protein-protein interaction databases

BioGridi35107. 24 interactions.
DIPiDIP-5949N.
IntActiQ04958. 16 interactions.
MINTiMINT-653305.
STRINGi4932.YML059C.

Structurei

3D structure databases

ProteinModelPortaliQ04958.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1373 – 1537165PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1404 – 14085GXSXG

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi259 – 29739Asp-richAdd
BLAST
Compositional biasi713 – 7186Poly-Ser

Sequence similaritiesi

Belongs to the NTE family.
Contains 1 patatin domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0664.
GeneTreeiENSGT00390000002533.
HOGENOMiHOG000048680.
KOiK14676.
OMAiSPWTQTC.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04958-1 [UniParc]FASTAAdd to Basket

« Hide

MRSMNCTTNN TNNTGQNTKN SLGSSFNSSN YTSYRFQTCL TDQIISEAQT     50
WSLSSLFNFS WVVSYFVMGA SRMIFRYGWY LATLSLLRIP KWIFFKLHHV 100
QFTLSFWLIL FALAVIVFVT YTIMKERILS QYKRLTPEFL PLENTGKSGS 150
SANINAASTQ SANAPPAIGS STTGASSIID SKKHSLKDGN ENETFLSSYL 200
DQFLSAIKIF GYLEKPVFHD LTKNMKTQKM DEGEILLLDS TIGFAIVVEG 250
TLQLYHEVDH SDKDHGDETD HSDTDGLDDQ DRDEEDEEED DDIDNYDTKS 300
CSSNLIDEED ESVGYIHLKN GLGNFQLLNT VKPGNPLTSL VSILNLFTHS 350
MSSYGNSNFP SELSSPIDTT VSVNNMFCSS EQNFSNTDSM TNSTNSFPTF 400
PSSMPKLVAR AATDCTIGII PPQSFAKLTA KYPRSASHII QMVLTKLYHV 450
TFQTAHDYLG LTKEIMDIEV LLNKSIVYEL PYYLKEAVIR KFKTVDKSSG 500
SADLEPKPKN SNASSKLKKP PKAKPSDGII QSLKIANANA NTSSNSLSLK 550
PEFTHHPSSR HVVLGSRDQF NPGDLLSNVP LSRTMDILSP NPIHNNNRNK 600
SNGINTSTSN QHKRSSRSSS NNASVHSKKF SSLSPELRNA QLSTSPLSLD 650
NTSVHDHIHP SPVHLKGRVS PRPNLLPTTS FSAAQEETED SALRMALVEA 700
MLTYLGVNKS NMSVSSSSIA NMSSLNSPQL NEMYSRRPSN ASFLMSPHCT 750
PSDISVASSF ASPQTQPTML RILPKEYTIS NKRHNKSKSQ DKKKPRAYKE 800
ELTPNLDFED VKKDFAQGIQ LKFFKKGTTI VEQNARGKGL FYIISGKVNV 850
TTNSSSSVVS SMSKPEQVSA QSSHKGENPH HTQHLLYSVG SGGIVGYLSS 900
LIGYKSFVNI VAKSDVYVGF LSSATLERLF DKYFLIYLRI SDSLTKLLSS 950
RLLKLDHALE WVHLRASETL FSQGDSANGI YVVLNGRLRQ LQQQSLSNSN 1000
TSSEEVETQN IILGELAQGE SFGEVEVLTA MNRYSTIVAV RDSELARIPR 1050
TLFELLALEH PSIMIRVSRL VAKKIVGDRT VPALTGDPLS IKENDFTSLI 1100
PPTKASYSSS LSHKPQNITS GTITFRTITI LPITSGLPVE AFAMKLVQAF 1150
KQVGRTTIGL NQRTTLTHLG RHAFDRLSKL KQSGYFAELE EMYQTVVYIS 1200
DTPVKSNWTR TCIAQGDCIL LLADARSPSA EIGEYEKLLL NSKTTARTEL 1250
ILLHPERYVE PGLTHKWLRY RPWVHSHHHI QFSLTGTTLM NEGKMHVLNN 1300
GALALMDKLI QTEFSRKTQQ NISKLLPDSI KNTVENFSSR FMKSKRQYYT 1350
PVHRHKNDFL RLARILSGQA IGLVLGGGGA RGISHLGVIQ AIEEQGIPVD 1400
VIGGTSIGSF VGGLYAKDYD LVPIYGRVKK FAGRISSIWR MLTDLTWPVT 1450
SYTTGHEFNR GIWKTFGDTR IEDFWIQYYC NSTNITDSVQ EIHSFGYAWR 1500
YIRASMSLAG LLPPLEENGS MLLDGGYVDN LPVTEMRARG CQTIFAVDVG 1550
SADDRTPMEY GDSLNGFWII FNRWNPFSSH PNIPNMAEIQ VRLGYVASVN 1600
ALEKAKNTPG VVYVRPPIEE YATLDFSKFE EIYHVGVDYG RIFLQGLIDD 1650
DKMPYIPGSQ ETTLNSQVPE FLLHRRNSI 1679
Length:1,679
Mass (Da):187,133
Last modified:November 1, 1997 - v1
Checksum:i550FFCD4ACAF8E25
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46729 Genomic DNA. Translation: CAA86716.1.
BK006946 Genomic DNA. Translation: DAA09838.1.
PIRiS49802.
RefSeqiNP_013652.1. NM_001182418.1.

Genome annotation databases

EnsemblFungiiYML059C; YML059C; YML059C.
GeneIDi854943.
KEGGisce:YML059C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46729 Genomic DNA. Translation: CAA86716.1 .
BK006946 Genomic DNA. Translation: DAA09838.1 .
PIRi S49802.
RefSeqi NP_013652.1. NM_001182418.1.

3D structure databases

ProteinModelPortali Q04958.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35107. 24 interactions.
DIPi DIP-5949N.
IntActi Q04958. 16 interactions.
MINTi MINT-653305.
STRINGi 4932.YML059C.

Proteomic databases

MaxQBi Q04958.
PaxDbi Q04958.
PeptideAtlasi Q04958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML059C ; YML059C ; YML059C .
GeneIDi 854943.
KEGGi sce:YML059C.

Organism-specific databases

CYGDi YML059c.
SGDi S000004524. NTE1.

Phylogenomic databases

eggNOGi COG0664.
GeneTreei ENSGT00390000002533.
HOGENOMi HOG000048680.
KOi K14676.
OMAi SPWTQTC.
OrthoDBi EOG70W3NS.

Enzyme and pathway databases

BioCyci MetaCyc:G3O-32654-MONOMER.
YEAST:G3O-32654-MONOMER.

Miscellaneous databases

NextBioi 977998.
PROi Q04958.

Gene expression databases

Genevestigatori Q04958.

Family and domain databases

Gene3Di 2.60.120.10. 5 hits.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells."
    Zaccheo O., Dinsdale D., Meacock P.A., Glynn P.
    J. Biol. Chem. 279:24024-24033(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, MUTAGENESIS OF SER-1406.
  6. "Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p."
    Fernandez-Murray J.P., McMaster C.R.
    J. Biol. Chem. 280:8544-8552(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  7. "Inositol induces a profound alteration in the pattern and rate of synthesis and turnover of membrane lipids in Saccharomyces cerevisiae."
    Gaspar M.L., Aregullin M.A., Jesch S.A., Henry S.A.
    J. Biol. Chem. 281:22773-22785(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634 AND THR-803, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-312; SER-632; SER-634; SER-653; SER-661; SER-670; SER-680 AND SER-739, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNTE1_YEAST
AccessioniPrimary (citable) accession number: Q04958
Secondary accession number(s): D6VZB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi