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Q04958

- NTE1_YEAST

UniProt

Q04958 - NTE1_YEAST

Protein

Lysophospholipase NTE1

Gene

NTE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium.3 Publications

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Positively regulated by SEC14. Inhibited by organophosphorus esters in the order phenyl saligenin phosphate (PSP) > phenyldipentyl phosphinate (PDPP) = diisopropyl fluorophosphate (DFP) > and paraoxon (PXN).2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei1406 – 14061

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi803 – 947145cNMP 1Add
    BLAST
    Nucleotide bindingi943 – 1074132cNMP 2Add
    BLAST

    GO - Molecular functioni

    1. lysophospholipase activity Source: SGD

    GO - Biological processi

    1. phosphatidylcholine catabolic process Source: SGD
    2. regulation of phospholipid biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-32654-MONOMER.
    YEAST:G3O-32654-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipase NTE1 (EC:3.1.1.5)
    Alternative name(s):
    Intracellular phospholipase B
    Neuropathy target esterase homolog
    Gene namesi
    Name:NTE1
    Ordered Locus Names:YML059C
    ORF Names:YM9958.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML059c.
    SGDiS000004524. NTE1.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1406 – 14061S → A: Loss of esterase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16791679Lysophospholipase NTE1PRO_0000172528Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei300 – 3001Phosphoserine1 Publication
    Modified residuei312 – 3121Phosphoserine1 Publication
    Modified residuei632 – 6321Phosphoserine1 Publication
    Modified residuei634 – 6341Phosphoserine3 Publications
    Modified residuei653 – 6531Phosphoserine1 Publication
    Modified residuei661 – 6611Phosphoserine1 Publication
    Modified residuei670 – 6701Phosphoserine1 Publication
    Modified residuei680 – 6801Phosphoserine1 Publication
    Modified residuei739 – 7391Phosphoserine1 Publication
    Modified residuei803 – 8031Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ04958.
    PaxDbiQ04958.
    PeptideAtlasiQ04958.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04958.

    Interactioni

    Protein-protein interaction databases

    BioGridi35107. 24 interactions.
    DIPiDIP-5949N.
    IntActiQ04958. 16 interactions.
    MINTiMINT-653305.
    STRINGi4932.YML059C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04958.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4949CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini71 – 10333LumenalSequence AnalysisAdd
    BLAST
    Topological domaini125 – 16791555CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei104 – 12421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1373 – 1537165PatatinAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1404 – 14085GXSXG

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi259 – 29739Asp-richAdd
    BLAST
    Compositional biasi713 – 7186Poly-Ser

    Sequence similaritiesi

    Belongs to the NTE family.Curated
    Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
    Contains 1 patatin domain.Curated

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0664.
    GeneTreeiENSGT00390000002533.
    HOGENOMiHOG000048680.
    KOiK14676.
    OMAiSPWTQTC.
    OrthoDBiEOG70W3NS.

    Family and domain databases

    Gene3Di2.60.120.10. 5 hits.
    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR001423. LysoPLipase_patatin_CS.
    IPR002641. Patatin/PLipase_A2-rel.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 2 hits.
    PF01734. Patatin. 1 hit.
    [Graphical view]
    SMARTiSM00100. cNMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 4 hits.
    SSF52151. SSF52151. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
    PS01237. UPF0028. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04958-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSMNCTTNN TNNTGQNTKN SLGSSFNSSN YTSYRFQTCL TDQIISEAQT     50
    WSLSSLFNFS WVVSYFVMGA SRMIFRYGWY LATLSLLRIP KWIFFKLHHV 100
    QFTLSFWLIL FALAVIVFVT YTIMKERILS QYKRLTPEFL PLENTGKSGS 150
    SANINAASTQ SANAPPAIGS STTGASSIID SKKHSLKDGN ENETFLSSYL 200
    DQFLSAIKIF GYLEKPVFHD LTKNMKTQKM DEGEILLLDS TIGFAIVVEG 250
    TLQLYHEVDH SDKDHGDETD HSDTDGLDDQ DRDEEDEEED DDIDNYDTKS 300
    CSSNLIDEED ESVGYIHLKN GLGNFQLLNT VKPGNPLTSL VSILNLFTHS 350
    MSSYGNSNFP SELSSPIDTT VSVNNMFCSS EQNFSNTDSM TNSTNSFPTF 400
    PSSMPKLVAR AATDCTIGII PPQSFAKLTA KYPRSASHII QMVLTKLYHV 450
    TFQTAHDYLG LTKEIMDIEV LLNKSIVYEL PYYLKEAVIR KFKTVDKSSG 500
    SADLEPKPKN SNASSKLKKP PKAKPSDGII QSLKIANANA NTSSNSLSLK 550
    PEFTHHPSSR HVVLGSRDQF NPGDLLSNVP LSRTMDILSP NPIHNNNRNK 600
    SNGINTSTSN QHKRSSRSSS NNASVHSKKF SSLSPELRNA QLSTSPLSLD 650
    NTSVHDHIHP SPVHLKGRVS PRPNLLPTTS FSAAQEETED SALRMALVEA 700
    MLTYLGVNKS NMSVSSSSIA NMSSLNSPQL NEMYSRRPSN ASFLMSPHCT 750
    PSDISVASSF ASPQTQPTML RILPKEYTIS NKRHNKSKSQ DKKKPRAYKE 800
    ELTPNLDFED VKKDFAQGIQ LKFFKKGTTI VEQNARGKGL FYIISGKVNV 850
    TTNSSSSVVS SMSKPEQVSA QSSHKGENPH HTQHLLYSVG SGGIVGYLSS 900
    LIGYKSFVNI VAKSDVYVGF LSSATLERLF DKYFLIYLRI SDSLTKLLSS 950
    RLLKLDHALE WVHLRASETL FSQGDSANGI YVVLNGRLRQ LQQQSLSNSN 1000
    TSSEEVETQN IILGELAQGE SFGEVEVLTA MNRYSTIVAV RDSELARIPR 1050
    TLFELLALEH PSIMIRVSRL VAKKIVGDRT VPALTGDPLS IKENDFTSLI 1100
    PPTKASYSSS LSHKPQNITS GTITFRTITI LPITSGLPVE AFAMKLVQAF 1150
    KQVGRTTIGL NQRTTLTHLG RHAFDRLSKL KQSGYFAELE EMYQTVVYIS 1200
    DTPVKSNWTR TCIAQGDCIL LLADARSPSA EIGEYEKLLL NSKTTARTEL 1250
    ILLHPERYVE PGLTHKWLRY RPWVHSHHHI QFSLTGTTLM NEGKMHVLNN 1300
    GALALMDKLI QTEFSRKTQQ NISKLLPDSI KNTVENFSSR FMKSKRQYYT 1350
    PVHRHKNDFL RLARILSGQA IGLVLGGGGA RGISHLGVIQ AIEEQGIPVD 1400
    VIGGTSIGSF VGGLYAKDYD LVPIYGRVKK FAGRISSIWR MLTDLTWPVT 1450
    SYTTGHEFNR GIWKTFGDTR IEDFWIQYYC NSTNITDSVQ EIHSFGYAWR 1500
    YIRASMSLAG LLPPLEENGS MLLDGGYVDN LPVTEMRARG CQTIFAVDVG 1550
    SADDRTPMEY GDSLNGFWII FNRWNPFSSH PNIPNMAEIQ VRLGYVASVN 1600
    ALEKAKNTPG VVYVRPPIEE YATLDFSKFE EIYHVGVDYG RIFLQGLIDD 1650
    DKMPYIPGSQ ETTLNSQVPE FLLHRRNSI 1679
    Length:1,679
    Mass (Da):187,133
    Last modified:November 1, 1997 - v1
    Checksum:i550FFCD4ACAF8E25
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46729 Genomic DNA. Translation: CAA86716.1.
    BK006946 Genomic DNA. Translation: DAA09838.1.
    PIRiS49802.
    RefSeqiNP_013652.1. NM_001182418.1.

    Genome annotation databases

    EnsemblFungiiYML059C; YML059C; YML059C.
    GeneIDi854943.
    KEGGisce:YML059C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46729 Genomic DNA. Translation: CAA86716.1 .
    BK006946 Genomic DNA. Translation: DAA09838.1 .
    PIRi S49802.
    RefSeqi NP_013652.1. NM_001182418.1.

    3D structure databases

    ProteinModelPortali Q04958.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35107. 24 interactions.
    DIPi DIP-5949N.
    IntActi Q04958. 16 interactions.
    MINTi MINT-653305.
    STRINGi 4932.YML059C.

    Proteomic databases

    MaxQBi Q04958.
    PaxDbi Q04958.
    PeptideAtlasi Q04958.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML059C ; YML059C ; YML059C .
    GeneIDi 854943.
    KEGGi sce:YML059C.

    Organism-specific databases

    CYGDi YML059c.
    SGDi S000004524. NTE1.

    Phylogenomic databases

    eggNOGi COG0664.
    GeneTreei ENSGT00390000002533.
    HOGENOMi HOG000048680.
    KOi K14676.
    OMAi SPWTQTC.
    OrthoDBi EOG70W3NS.

    Enzyme and pathway databases

    BioCyci MetaCyc:G3O-32654-MONOMER.
    YEAST:G3O-32654-MONOMER.

    Miscellaneous databases

    NextBioi 977998.
    PROi Q04958.

    Gene expression databases

    Genevestigatori Q04958.

    Family and domain databases

    Gene3Di 2.60.120.10. 5 hits.
    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR001423. LysoPLipase_patatin_CS.
    IPR002641. Patatin/PLipase_A2-rel.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 2 hits.
    PF01734. Patatin. 1 hit.
    [Graphical view ]
    SMARTi SM00100. cNMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 4 hits.
    SSF52151. SSF52151. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
    PS01237. UPF0028. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells."
      Zaccheo O., Dinsdale D., Meacock P.A., Glynn P.
      J. Biol. Chem. 279:24024-24033(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, MUTAGENESIS OF SER-1406.
    6. "Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p."
      Fernandez-Murray J.P., McMaster C.R.
      J. Biol. Chem. 280:8544-8552(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION.
    7. "Inositol induces a profound alteration in the pattern and rate of synthesis and turnover of membrane lipids in Saccharomyces cerevisiae."
      Gaspar M.L., Aregullin M.A., Jesch S.A., Henry S.A.
      J. Biol. Chem. 281:22773-22785(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634 AND THR-803, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-312; SER-632; SER-634; SER-653; SER-661; SER-670; SER-680 AND SER-739, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNTE1_YEAST
    AccessioniPrimary (citable) accession number: Q04958
    Secondary accession number(s): D6VZB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 521 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3