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Protein

DNA polymerase I

Gene

polA

Organism
Bacillus caldotenax
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
OrganismiBacillus caldotenax
Taxonomic identifieri1395 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877DNA polymerase IPRO_0000101233Add
BLAST

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.

Structurei

Secondary structure

1
877
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi309 – 3124
Beta strandi314 – 3218
Beta strandi324 – 3263
Beta strandi334 – 3396
Beta strandi342 – 3465
Helixi348 – 3514
Helixi355 – 3628
Beta strandi366 – 3727
Helixi373 – 3819
Turni382 – 3843
Beta strandi390 – 3934
Helixi394 – 4018
Helixi403 – 4053
Helixi410 – 4156
Turni416 – 4183
Helixi425 – 4295
Helixi432 – 4343
Helixi440 – 46728
Helixi471 – 4766
Helixi478 – 49114
Beta strandi493 – 4953
Helixi497 – 52226
Helixi531 – 5399
Helixi558 – 5647
Helixi565 – 5673
Helixi570 – 5756
Helixi577 – 58610
Turni587 – 5893
Helixi590 – 5934
Turni598 – 6003
Beta strandi606 – 6105
Beta strandi618 – 6225
Helixi632 – 6354
Helixi636 – 6405
Beta strandi648 – 65710
Helixi658 – 66710
Helixi670 – 6778
Helixi682 – 69110
Helixi695 – 6973
Helixi700 – 71516
Helixi719 – 7268
Helixi730 – 74314
Helixi745 – 76117
Beta strandi762 – 7654
Beta strandi771 – 7733
Helixi775 – 7784
Helixi782 – 81837
Beta strandi824 – 8285
Beta strandi830 – 8389
Helixi839 – 8413
Helixi842 – 85413
Beta strandi865 – 8728
Turni873 – 8753

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HHXX-ray2.26A298-877[»]
ProteinModelPortaliQ04957.
SMRiQ04957. Positions 297-877.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04957.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 3103105'-3' exonucleaseAdd
BLAST
Domaini311 – 4651553'-5' exonucleaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni469 – 877409PolymeraseBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 3'-5' exonuclease domain.Curated
Contains 1 5'-3' exonuclease domain.Curated

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKLVLIDG SSVAYRAFFA LPLLHNDKGI HTNAVYGFTM MLNKILAEEE
60 70 80 90 100
PTHMLVAFDA GKTTFRHEAF QEYKGGRQQT PPELSEQFPL LRELLRAYRI
110 120 130 140 150
PAYELENYEA DDIIGTLAAR AEQEGFEVKV ISGDRDLTQL ASPHVTVDIT
160 170 180 190 200
KKGITDIEPY TPEAVREKYG LTPEQIVDLK GLMGDKSDNI PGVPGIGEKT
210 220 230 240 250
AVKLLRQFGT VENVLASIDE IKGEKLKETL RQHREMALLS KKLAAIRRDA
260 270 280 290 300
PVELSLDDIA YQGEDREKVV ALFKELGFQS FLEKMESPSS EEEKPLAKMA
310 320 330 340 350
FTLADRVTEE MLADKAALVV EVVEENYHDA PIVGIAVVNE HGRFFLRPET
360 370 380 390 400
ALADPQFVAW LGDETKKKSM FDSKRAAVAL KWKGIELCGV SFDLLLAAYL
410 420 430 440 450
LDPAQGVDDV AAAAKMKQYE AVRPDEAVYG KGAKRAVPDE PVLAEHLVRK
460 470 480 490 500
AAAIWALERP FLDELRRNEQ DRLLVELEQP LSSILAEMEF AGVKVDTKRL
510 520 530 540 550
EQMGEELAEQ LRTVEQRIYE LAGQEFNINS PKQLGVILFE KLQLPVLKKS
560 570 580 590 600
KTGYSTSADV LEKLAPYHEI VENILQHYRQ LGKLQSTYIE GLLKVVRPDT
610 620 630 640 650
KKVHTIFNQA LTQTGRLSST EPNLQNIPIR LEEGRKIRQA FVPSESDWLI
660 670 680 690 700
FAADYSQIEL RVLAHIAEDD NLMEAFRRDL DIHTKTAMDI FQVSEDEVTP
710 720 730 740 750
NMRRQAKAVN FGIVYGISDY GLAQNLNISR KEAAEFIERY FESFPGVKRY
760 770 780 790 800
MENIVQEAKQ KGYVTTLLHR RRYLPDITSR NFNVRSFAER MAMNTPIQGS
810 820 830 840 850
AADIIKKAMI DLNARLKEER LQARLLLQVH DELILEAPKE EMERLCRLVP
860 870
EVMEQAVTLR VPLKVDYHYG STWYDAK
Length:877
Mass (Da):99,475
Last modified:October 1, 1993 - v1
Checksum:iDA5FC7F5B6DFA6F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12982 Genomic DNA. Translation: BAA02361.1.
PIRiJX0256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12982 Genomic DNA. Translation: BAA02361.1.
PIRiJX0256.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HHXX-ray2.26A298-877[»]
ProteinModelPortaliQ04957.
SMRiQ04957. Positions 297-877.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ04957.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO1_BACCA
AccessioniPrimary (citable) accession number: Q04957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 14, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.