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Protein

1,3-beta-glucan synthase component FKS3

Gene

FKS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for spore wall assembly.1 Publication

Catalytic activityi

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

GO - Molecular functioni

  • 1,3-beta-D-glucan synthase activity Source: SGD

GO - Biological processi

  • (1->3)-beta-D-glucan biosynthetic process Source: InterPro
  • ascospore wall assembly Source: SGD
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32970-MONOMER.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
1,3-beta-glucan synthase component FKS3 (EC:2.4.1.34)
Alternative name(s):
1,3-beta-D-glucan-UDP glucosyltransferase
FK506 sensitivity protein 3
Gene namesi
Name:FKS3
Ordered Locus Names:YMR306W
ORF Names:YM9952.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR306W.
SGDiS000004923. FKS3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei337 – 35721HelicalSequence analysisAdd
BLAST
Transmembranei375 – 39521HelicalSequence analysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence analysisAdd
BLAST
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Transmembranei508 – 52821HelicalSequence analysisAdd
BLAST
Transmembranei547 – 56721HelicalSequence analysisAdd
BLAST
Transmembranei572 – 59221HelicalSequence analysisAdd
BLAST
Transmembranei712 – 73221HelicalSequence analysisAdd
BLAST
Transmembranei1215 – 123521HelicalSequence analysisAdd
BLAST
Transmembranei1268 – 128821HelicalSequence analysisAdd
BLAST
Transmembranei1303 – 132321HelicalSequence analysisAdd
BLAST
Transmembranei1370 – 139021HelicalSequence analysisAdd
BLAST
Transmembranei1394 – 141421HelicalSequence analysisAdd
BLAST
Transmembranei1475 – 149521HelicalSequence analysisAdd
BLAST
Transmembranei1514 – 153421HelicalSequence analysisAdd
BLAST
Transmembranei1549 – 156921HelicalSequence analysisAdd
BLAST
Transmembranei1585 – 160521HelicalSequence analysisAdd
BLAST
Transmembranei1655 – 167521HelicalSequence analysisAdd
BLAST
Transmembranei1713 – 173321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178517851,3-beta-glucan synthase component FKS3PRO_0000121727Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi844 – 8441N-linked (GlcNAc...)Sequence analysis
Glycosylationi874 – 8741N-linked (GlcNAc...)Sequence analysis
Glycosylationi955 – 9551N-linked (GlcNAc...)Sequence analysis
Glycosylationi1002 – 10021N-linked (GlcNAc...)Sequence analysis
Glycosylationi1170 – 11701N-linked (GlcNAc...)Sequence analysis
Glycosylationi1360 – 13601N-linked (GlcNAc...)Sequence analysis
Glycosylationi1579 – 15791N-linked (GlcNAc...)Sequence analysis
Glycosylationi1761 – 17611N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

PTM databases

iPTMnetiQ04952.

Interactioni

Protein-protein interaction databases

BioGridi35486. 18 interactions.
DIPiDIP-7253N.
IntActiQ04952. 3 interactions.
MINTiMINT-1359239.

Structurei

3D structure databases

ProteinModelPortaliQ04952.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004828.
HOGENOMiHOG000216604.
InParanoidiQ04952.
KOiK00706.
OMAiIKLLYRI.
OrthoDBiEOG7JT74G.

Family and domain databases

InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFMSPKFSL TDVEYPAWCQ DDEVPITMQE IREIFVELMD KFGFQKSSME
60 70 80 90 100
NMYQHLMGQL DSRASRTGAQ NALVSLHVSY IGGEHANYRK WYFAAQLDLD
110 120 130 140 150
EEIGFQNMRL HGKARQRNVK MAKKRGVSIK EQIKQWNEKE QEFINNHPKI
160 170 180 190 200
TLTQEQLEDQ TNLKSADYKW KLKMKKLTPE NMIRQLALYL LCWGEANQVR
210 220 230 240 250
FAPECLCFIF KCALDYDIST SSSEKTVKSP EYSYLNDVIT PLYEFLRGQV
260 270 280 290 300
YKKDAKGNWK RREKDHKNII GYDDINQLFW YPEGFERIIL NNGERLVDKP
310 320 330 340 350
LEERYLYFKD VAWSKVFYKT YRETRSWKHC FTNFNRFWII HFAPFWFFTT
360 370 380 390 400
FNSPTLYTKN YIQLLNNQPT PQVRLSVIAF GGTIACLVQI LATVFEWGFV
410 420 430 440 450
PREWPGAQHL SSRMIGLLFC LAINLGPSVY VLGFFEWDVH SKSAYIVSIV
460 470 480 490 500
QLIIAFLTTF FFAVRPLGGL FRPYLNKDKK HRRYISSQTF TASFPKLTGR
510 520 530 540 550
SKWFSYGLWV FVYLAKYIES YFFLTLSLRD PIRVLSIMDL SRCQGEYLLG
560 570 580 590 600
PILCKWQAKI TLVLMLLSDL GLFFLDTYLW YIICNCIFSI VLSFSLGTSI
610 620 630 640 650
LTPWKNVYSR LPKRIYSKIL ATSEMDVKFK AKILISQVWN AIVISMYREH
660 670 680 690 700
LLSIEHLQRL LFQQVDSLMG DTRTLKSPTF FVAQDDSTFK SMEFFPSNSE
710 720 730 740 750
AKRRISFFAQ SLATPISEPV PVDCMPTFTV LVPHYSEKIL LGLKEIIREE
760 770 780 790 800
SPKSKITVLE YLKHLHPTEW ECFVKDTKLL SMEKSFLKEA ESSHDEDRLE
810 820 830 840 850
IPDALYDPRS SPLSDHTESR KLPTEDDLIK EKINDLPFSY FGFNSSEPSY
860 870 880 890 900
TLRTRIWASL RTQTLYRTLS GFMNYSKAIK LLYRIENPSL VSLYRGNNEA
910 920 930 940 950
LENDLENMAS RKFRMVVAMQ RYAKFNKDEV EATELLLRAY PNMFISYLLE
960 970 980 990 1000
ELEQNESEKT YYSCLTNGYA EFDEESGLRK PIFKIRLSGN PILGDGKSDN
1010 1020 1030 1040 1050
QNHSIIFYRG EYIQVIDANQ DNYLEECLKI RSVLSEFEEL ELNPTIPYIP
1060 1070 1080 1090 1100
GIEYEEEPPP IAIVGSREYI FSENIGVLGD IAAGKEQTFG TLFARTLAEI
1110 1120 1130 1140 1150
GGKLHYGHPD FLNGIFMTTR GGLSKAQRGL HLNEDIYAGM NAICRGGKIK
1160 1170 1180 1190 1200
HSDYYQCGKG RDLGFGSILN FTTKIGAGMG EQLLSREYYY LGTQLPMDRF
1210 1220 1230 1240 1250
LSFFYAHPGF HLNNLFISFS VQLFFVLLLN LGALNHEIIA CFYDKDAPIT
1260 1270 1280 1290 1300
NLETPVGCYN IQPALHWVSI FVLSIFIVFF IAFAPLLIQE VLEKGIWRAA
1310 1320 1330 1340 1350
SRFLHHLLSM APLFEVFVCQ VYSNSLLMDL TFGGAKYIST GRGFAITRLD
1360 1370 1380 1390 1400
FFTLYSRFVN ISIYSGFQVF FMLLFAIISM WQPALLWFWI TVISMCFAPF
1410 1420 1430 1440 1450
IFNPHQFAFM DFFIDYKTFI HWLFSGNTKY QKESWANFVK SSRSRFTGYK
1460 1470 1480 1490 1500
SKTVDDISED SGHDSKKARF WNVFFAELFL PFCVFLFNFT AFSFINAQTG
1510 1520 1530 1540 1550
VSDSTPTSAV FRLLLVTFLP IFLNSIVLFL LFWVSLFVVP GLSYCCKDAG
1560 1570 1580 1590 1600
AVIAFIAHTF SVLVYLLDFE LMWFLQGWNF TRTLILLITC INMHLILFKV
1610 1620 1630 1640 1650
FTTIFLTREY KNNKAHLAWW NGKWYNTGMG WSIILQPIRE YFVKIMESSY
1660 1670 1680 1690 1700
FAADFFLGHF LLFIQTPIIL LPFIDYWHTM VLFWMNPRSI IAHKRILTRK
1710 1720 1730 1740 1750
QRALRSRIVS KYFSLYFVML GVLLFMLIAP FFAGDFVSSP QELLEGTLFE
1760 1770 1780
GIFQPNNQNN NDTGPNAPST ILTTTPTLPT FRTVA
Length:1,785
Mass (Da):207,483
Last modified:November 1, 1997 - v1
Checksum:i3475446DA46C6120
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA. Translation: CAA89139.1.
BK006946 Genomic DNA. Translation: DAA10207.1.
PIRiS53976.
RefSeqiNP_014036.1. NM_001182817.1.

Genome annotation databases

EnsemblFungiiYMR306W; YMR306W; YMR306W.
GeneIDi855353.
KEGGisce:YMR306W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA. Translation: CAA89139.1.
BK006946 Genomic DNA. Translation: DAA10207.1.
PIRiS53976.
RefSeqiNP_014036.1. NM_001182817.1.

3D structure databases

ProteinModelPortaliQ04952.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35486. 18 interactions.
DIPiDIP-7253N.
IntActiQ04952. 3 interactions.
MINTiMINT-1359239.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.

PTM databases

iPTMnetiQ04952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR306W; YMR306W; YMR306W.
GeneIDi855353.
KEGGisce:YMR306W.

Organism-specific databases

EuPathDBiFungiDB:YMR306W.
SGDiS000004923. FKS3.

Phylogenomic databases

GeneTreeiENSGT00390000004828.
HOGENOMiHOG000216604.
InParanoidiQ04952.
KOiK00706.
OMAiIKLLYRI.
OrthoDBiEOG7JT74G.

Enzyme and pathway databases

BioCyciYEAST:G3O-32970-MONOMER.

Miscellaneous databases

PROiQ04952.

Family and domain databases

InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  4. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "Homologous subunits of 1,3-beta-glucan synthase are important for spore wall assembly in Saccharomyces cerevisiae."
    Ishihara S., Hirata A., Nogami S., Beauvais A., Latge J.-P., Ohya Y.
    Eukaryot. Cell 6:143-156(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
    Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
    Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFKS3_YEAST
AccessioniPrimary (citable) accession number: Q04952
Secondary accession number(s): D6W0D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.