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Protein

Probable family 17 glucosidase SCW10

Gene

SCW10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei326 – 3261NucleophileBy similarity
Active sitei380 – 3801Proton donorBy similarity

GO - Molecular functioni

  • glucosidase activity Source: SGD

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YMR305C-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable family 17 glucosidase SCW10 (EC:3.2.1.-)
Alternative name(s):
Soluble cell wall protein 10
Gene namesi
Name:SCW10
Ordered Locus Names:YMR305C
ORF Names:YM9952.07C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR305C.
SGDiS000004921. SCW10.

Subcellular locationi

  • Secretedcell wall 1 Publication

GO - Cellular componenti

  • extracellular region Source: SGD
  • fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Propeptidei19 – 29111 PublicationPRO_0000011901Add
BLAST
Chaini30 – 389360Probable family 17 glucosidase SCW10PRO_0000011902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Proteomic databases

MaxQBiQ04951.
PeptideAtlasiQ04951.

Miscellaneous databases

PMAP-CutDBQ04951.

Interactioni

Protein-protein interaction databases

BioGridi35485. 19 interactions.
DIPiDIP-3845N.
MINTiMINT-574800.

Structurei

3D structure databases

ProteinModelPortaliQ04951.
SMRiQ04951. Positions 138-389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi65 – 13470Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000008245.
HOGENOMiHOG000216601.
InParanoidiQ04951.
OMAiFITETGW.
OrthoDBiEOG73FQWG.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFSNFLTVS ALLTGALGAP AVRHKHEKRD VVTATVHAQV TVVVSGNSGE
60 70 80 90 100
TIVPVNENAV VATTSSTAVA SQATTSTLEP TTSANVVTSQ QQTSTLQSSE
110 120 130 140 150
AASTVGSSTS SSPSSSSSTS SSASSSASSS ISASGAKGIT YSPYNDDGSC
160 170 180 190 200
KSTAQVASDL EQLTGFDNIR LYGVDCSQVE NVLQAKTSSQ KLFLGIYYVD
210 220 230 240 250
KIQDAVDTIK SAVESYGSWD DITTVSVGNE LVNGGSATTT QVGEYVSTAK
260 270 280 290 300
SALTSAGYTG SVVSVDTFIA VINNPDLCNY SDYMAVNAHA YFDENTAAQD
310 320 330 340 350
AGPWVLEQIE RVYTACGGKK DVVITETGWP SKGDTYGEAV PSKANQEAAI
360 370 380
SSIKSSCGSS AYLFTAFNDL WKDDGQYGVE KYWGILSSD
Length:389
Mass (Da):40,469
Last modified:November 1, 1997 - v1
Checksum:i21F394BD41337DE1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 392AQ → QE AA sequence (PubMed:9748433).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA. Translation: CAA89138.1.
BK006946 Genomic DNA. Translation: DAA10206.1.
PIRiS53975.
RefSeqiNP_014035.1. NM_001182815.1.

Genome annotation databases

EnsemblFungiiYMR305C; YMR305C; YMR305C.
GeneIDi855352.
KEGGisce:YMR305C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA. Translation: CAA89138.1.
BK006946 Genomic DNA. Translation: DAA10206.1.
PIRiS53975.
RefSeqiNP_014035.1. NM_001182815.1.

3D structure databases

ProteinModelPortaliQ04951.
SMRiQ04951. Positions 138-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35485. 19 interactions.
DIPiDIP-3845N.
MINTiMINT-574800.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Proteomic databases

MaxQBiQ04951.
PeptideAtlasiQ04951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR305C; YMR305C; YMR305C.
GeneIDi855352.
KEGGisce:YMR305C.

Organism-specific databases

EuPathDBiFungiDB:YMR305C.
SGDiS000004921. SCW10.

Phylogenomic databases

GeneTreeiENSGT00390000008245.
HOGENOMiHOG000216601.
InParanoidiQ04951.
OMAiFITETGW.
OrthoDBiEOG73FQWG.

Enzyme and pathway databases

BioCyciYEAST:YMR305C-MONOMER.

Miscellaneous databases

PMAP-CutDBQ04951.
PROiQ04951.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating."
    Cappellaro C., Mrsa V., Tanner W.
    J. Bacteriol. 180:5030-5037(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-39, SUBCELLULAR LOCATION.
    Strain: ATCC 96099 / S288c / SEY6210.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSCW10_YEAST
AccessioniPrimary (citable) accession number: Q04951
Secondary accession number(s): D6W0D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.