Q04947 (RTN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reticulon-like protein 1

Gene names

Name:	RTN1
Ordered Locus Names:	YDR233C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with POM33. Ref.11
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.6.
Miscellaneous	Present with 37105 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 1 reticulon domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	endoplasmic reticulum tubular network organization Inferred from mutant phenotype. Source: UniProtKB protein import into nucleus Inferred from genetic interaction. Source: SGD
Cellular component	Golgi apparatus Inferred from direct assay. Source: SGD cortical endoplasmic reticulum Inferred from direct assay. Source: UniProtKB endoplasmic reticulum tubular network Inferred from direct assay. Source: UniProtKB integral to cytosolic side of endoplasmic reticulum membrane Inferred from direct assay. Source: UniProtKB mitochondrion Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SEY1	Q99287	3	EBI-38020,EBI-37523

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Reticulon-like protein 1		PRO_0000168168
Regions
	Topological domain	1 – 50	50	Cytoplasmic Potential
	Transmembrane	51 – 73	23	Helical; Potential
	Topological domain	74 – 142	69	Lumenal Potential
	Transmembrane	143 – 163	21	Helical; Potential
	Topological domain	164 – 295	132	Cytoplasmic Potential
	Domain	20 – 220	201	Reticulon
	Coiled coil	265 – 295	31	Potential
	Compositional bias	9 – 16	8	Poly-Gln
Amino acid modifications
	Modified residue	186	1	Phosphothreonine Ref.9 Ref.10
	Modified residue	219	1	Phosphothreonine Ref.9
	Modified residue	230	1	Phosphothreonine Ref.10
	Modified residue	232	1	Phosphoserine Ref.10
	Modified residue	234	1	Phosphoserine Ref.10
	Modified residue	265	1	Phosphoserine Ref.10
	Modified residue	267	1	Phosphothreonine Ref.10
	Modified residue	276	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q04947 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 245575E65D3C2193	FASTA	295	32,916
	10 20 30 40 50 60 MSASAQHSQA QQQQQQKSCN CDLLLWRNPV QTGKYFGGSL LALLILKKVN LITFFLKVAY 70 80 90 100 110 120 TILFTTGSIE FVSKLFLGQG LITKYGPKEC PNIAGFIKPH IDEALKQLPV FQAHIRKTVF 130 140 150 160 170 180 AQVPKHTFKT AVALFLLHKF FSWFSIWTIV FVADIFTFTL PVIYHSYKHE IDATVAQGVE 190 200 210 220 230 240 ISKQKTQEFS QMACEKTKPY LDKVESKLGP ISNLVKSKTA PVSSTAGPQT ASTSKLAADV 250 260 270 280 290 PLEPESKAYT SSAQVMPEVP QHEPSTTQEF NVDELSNELK KSTKNLQNEL EKNNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A reticular rhapsody: phylogenic evolution and nomenclature of the RTN/Nogo gene family." Oertle T., Klinger M., Stuermer C.A.O., Schwab M.E. FASEB J. 17:1238-1247(2003) [PubMed: 12832288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Bienvenut W.V., Peters C. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 18-27; 219-247 AND 285-295, MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186 AND THR-219, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186; THR-230; SER-232; SER-234; SER-265; THR-267 AND SER-276, MASS SPECTROMETRY.
[11]	"Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution." Chadrin A., Hess B., San Roman M., Gatti X., Lombard B., Loew D., Barral Y., Palancade B., Doye V. J. Cell Biol. 189:795-811(2010) [PubMed: 20498018] [Abstract] Cited for: INTERACTION WITH POM33.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY164798 mRNA. Translation: AAP47373.1. Z48612 Genomic DNA. Translation: CAA88512.1. AY558579 Genomic DNA. Translation: AAS56905.1. BK006938 Genomic DNA. Translation: DAA12074.1.
PIR	S59439.
RefSeq	NP_010519.1. NM_001180541.1.
3D structure databases
ProteinModelPortal	Q04947.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4090N.
IntAct	Q04947. 98 interactions.
MINT	MINT-510849.
STRING	Q04947.
Proteomic databases
PeptideAtlas	Q04947.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR233C; YDR233C; YDR233C.
GeneID	851819.
KEGG	sce:YDR233C.
NMPDR	fig|4932.3.peg.1276.
Organism-specific databases
CYGD	YDR233c.
SGD	S000002641. RTN1.
Phylogenomic databases
eggNOG	fuNOG09841.
GeneTree	EFGT00050000006485.
HOGENOM	HBG396976.
OMA	AFIFAAR.
OrthoDB	EOG44BFB7.
Gene expression databases
ArrayExpress	Q04947.
Genevestigator	Q04947.
GermOnline	YDR233C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013326. ApoA/E_ApoLp. IPR003388. Reticulon. [Graphical view]
Gene3D	G3DSA:1.20.120.20. ApoA/E_ApoLp. 2 hits.
PANTHER	PTHR10994. Reticulon. 1 hit.
Pfam	PF02453. Reticulon. 1 hit. [Graphical view]
PROSITE	PS50845. RETICULON. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969686.

Entry information

Entry name

RTN1_YEAST

Accession

Primary (citable) accession number: Q04947
Secondary accession number(s): D6VSL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program