Q04945 (ADHB_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-dependent butanol dehydrogenase B
EC=1.1.1.-

Alternative name(s):
BDH II

Gene names

Name:	bdhB
Ordered Locus Names:	CA_C3298

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Pathway	Alcohol metabolism; butanol biosynthesis.
Subunit structure	Homodimer.
Miscellaneous	Primarily NADH-dependent, although some NADPH-dependent activity was observed.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	NADH-dependent butanol dehydrogenase B		PRO_0000087823

Sequences

	Sequence		Length	Mass (Da)
	Q04945 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: BDAA32635C17CAB9	FASTA	390	43,287
	10 20 30 40 50 60 MVDFEYSIPT RIFFGKDKIN VLGRELKKYG SKVLIVYGGG SIKRNGIYDK AVSILEKNSI 70 80 90 100 110 120 KFYELAGVEP NPRVTTVEKG VKICRENGVE VVLAIGGGSA IDCAKVIAAA CEYDGNPWDI 130 140 150 160 170 180 VLDGSKIKRV LPIASILTIA ATGSEMDTWA VINNMDTNEK LIAAHPDMAP KFSILDPTYT 190 200 210 220 230 240 YTVPTNQTAA GTADIMSHIF EVYFSNTKTA YLQDRMAEAL LRTCIKYGGI ALEKPDDYEA 250 260 270 280 290 300 RANLMWASSL AINGLLTYGK DTNWSVHLME HELSAYYDIT HGVGLAILTP NWMEYILNND 310 320 330 340 350 360 TVYKFVEYGV NVWGIDKEKN HYDIAHQAIQ KTRDYFVNVL GLPSRLRDVG IEEEKLDIMA 370 380 390 KESVKLTGGT IGNLRPVNAS EVLQIFKKSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes." Walter K.A., Bennett G.N., Papoutsakis E.T. J. Bacteriol. 174:7149-7158(1992) [PubMed: 1385386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]	"Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum." Petersen D.J., Welch R.W., Walter K.A., Mermelstein L.D., Papoutsakis E.T., Rudolph F.B., Bennett G.N. Ann. N. Y. Acad. Sci. 646:94-98(1991) [PubMed: 1809209] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96946 Genomic DNA. Translation: AAA23207.1. AE001437 Genomic DNA. Translation: AAK81231.1.
PIR	B47013. D97305.
RefSeq	NP_349891.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q04945.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119480.
GenomeReviews	Gene locus CA_C3298 in contig AE001437_GR.
KEGG	cac:CA_C3298.
NMPDR	fig|272562.1.peg.3420.
PATRIC	32041023. VBICloAce74127_3476.
Phylogenomic databases
HOGENOM	HBG742415.
OMA	ILYAGTI.
PhylomeDB	Q04945.
ProtClustDB	CLSK2751995.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3298-MONOMER. MetaCyc:BDHBCLOS-MONOMER.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
KO	K00100.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADHB_CLOAB

Accession

Primary (citable) accession number: Q04945

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program