Q04944 (ADHA_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-dependent butanol dehydrogenase A
EC=1.1.1.-

Alternative name(s):
BDH I

Gene names

Name:	bdhA
Ordered Locus Names:	CA_C3299

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Pathway	Alcohol metabolism; butanol biosynthesis.
Subunit structure	Homodimer.
Miscellaneous	Primarily NADH-dependent, although some NADPH-dependent activity was observed.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	NADH-dependent butanol dehydrogenase A		PRO_0000087822
Experimental info
	Sequence conflict	217	1	A → R in AAA23206. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q04944 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 49E3B120F0E22252	FASTA	389	42,982
	10 20 30 40 50 60 MLSFDYSIPT KVFFGKGKID VIGEEIKKYG SRVLIVYGGG SIKRNGIYDR ATAILKENNI 70 80 90 100 110 120 AFYELSGVEP NPRITTVKKG IEICRENNVD LVLAIGGGSA IDCSKVIAAG VYYDGDTWDM 130 140 150 160 170 180 VKDPSKITKV LPIASILTLS ATGSEMDQIA VISNMETNEK LGVGHDDMRP KFSVLDPTYT 190 200 210 220 230 240 FTVPKNQTAA GTADIMSHTF ESYFSGVEGA YVQDGIAEAI LRTCIKYGKI AMEKTDDYEA 250 260 270 280 290 300 RANLMWASSL AINGLLSLGK DRKWSCHPME HELSAYYDIT HGVGLAILTP NWMEYILNDD 310 320 330 340 350 360 TLHKFVSYGI NVWGIDKNKD NYEIAREAIK NTREYFNSLG IPSKLREVGI GKDKLELMAK 370 380 QAVRNSGGTI GSLRPINAED VLEIFKKSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes." Walter K.A., Bennett G.N., Papoutsakis E.T. J. Bacteriol. 174:7149-7158(1992) [PubMed: 1385386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]	"Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum." Petersen D.J., Welch R.W., Walter K.A., Mermelstein L.D., Papoutsakis E.T., Rudolph F.B., Bennett G.N. Ann. N. Y. Acad. Sci. 646:94-98(1991) [PubMed: 1809209] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96945 Genomic DNA. Translation: AAA23206.1. AE001437 Genomic DNA. Translation: AAK81232.1.
PIR	A47013. E97305.
RefSeq	NP_349892.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q04944.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119481.
GenomeReviews	Gene locus CA_C3299 in contig AE001437_GR.
KEGG	cac:CA_C3299.
NMPDR	fig|272562.1.peg.3421.
PATRIC	32041025. VBICloAce74127_3477.
Phylogenomic databases
HOGENOM	HBG742415.
OMA	DINPEES.
PhylomeDB	Q04944.
ProtClustDB	CLSK2751995.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3299-MONOMER. MetaCyc:BDHACLOS-MONOMER.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
KO	K00100.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADHA_CLOAB

Accession

Primary (citable) accession number: Q04944

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program