ID   AFSQ2_STRCO             Reviewed;         535 AA.
AC   Q04943;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   16-JUN-2009, entry version 78.
DE   RecName: Full=Signal transduction histidine-protein kinase afsQ2;
DE            EC=2.7.13.3;
GN   Name=afsQ2; OrderedLocusNames=SCO4906; ORFNames=2SCK8.32c;
OS   Streptomyces coelicolor.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1902;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A3(2);
RX   MEDLINE=93077438; PubMed=1339426;
RA   Ishizuka H., Horinouchi S., Kieser H.M., Hopwood D.A., Beppu T.;
RT   "A putative two-component regulatory system involved in secondary
RT   metabolism in Streptomyces spp.";
RL   J. Bacteriol. 174:7585-7594(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   MEDLINE=21996410; PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H.,
RA   Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M.,
RA   Cronin A., Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S.,
RA   Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S.,
RA   Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S.,
RA   Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K.,
RA   Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J.,
RA   Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces
RT   coelicolor A3(2).";
RL   Nature 417:141-147(2002).
CC   -!- FUNCTION: Forms part of a two-component regulatory system
CC       afsQ1/afsQ2 involved in secondary metabolism. May activate afsQ1
CC       by phosphorylation.
CC   -!- CATALYTIC ACTIVITY: ATP + protein L-histidine = ADP + protein N-
CC       phospho-L-histidine.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Contains 1 HAMP domain.
CC   -!- SIMILARITY: Contains 1 histidine kinase domain.
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DR   EMBL; D10654; BAA01503.1; -; Genomic_DNA.
DR   EMBL; AL939121; CAC33071.1; -; Genomic_DNA.
DR   PIR; B45270; B45270.
DR   RefSeq; NP_629059.1; -.
DR   GeneID; 1100347; -.
DR   GenomeReviews; AL645882_GR; SCO4906.
DR   KEGG; sco:SCO4906; -.
DR   NMPDR; fig|100226.1.peg.4861; -.
DR   HOGENOM; Q04943; -.
DR   OMA; Q04943; LENAHIH.
DR   BioCyc; SCOE100226:SCO4906-MON; -.
DR   BRENDA; 2.7.13.3; 1084.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:two-component sensor activity; IEA:InterPro.
DR   GO; GO:0018106; P:peptidyl-histidine phosphorylation; IEA:InterPro.
DR   GO; GO:0000160; P:two-component signal transduction system (p...; IEA:UniProtKB-KW.
DR   InterPro; IPR003594; ATP_bd_ATPase.
DR   InterPro; IPR003660; HAMP_linker_domain.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   InterPro; IPR003661; Sig_transdc_His_kin_sub1_dim/P.
DR   InterPro; IPR005467; Sig_transdc_His_kinase_core.
DR   Gene3D; G3DSA:3.30.565.10; ATP_bd_ATPase; 1.
DR   Pfam; PF00672; HAMP; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00304; HAMP; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Complete proteome; Kinase; Membrane;
KW   Nucleotide-binding; Phosphoprotein; Transferase; Transmembrane;
KW   Two-component regulatory system.
FT   CHAIN         1    535       Signal transduction histidine-protein
FT                                kinase afsQ2.
FT                                /FTId=PRO_0000074683.
FT   TOPO_DOM      1     30       Cytoplasmic (Potential).
FT   TRANSMEM     31     52       Potential.
FT   TOPO_DOM     53    198       Extracellular (Potential).
FT   TRANSMEM    199    219       Potential.
FT   TOPO_DOM    220    535       Cytoplasmic (Potential).
FT   DOMAIN      224    276       HAMP.
FT   DOMAIN      291    510       Histidine kinase.
FT   MOD_RES     294    294       Phosphohistidine (By similarity).
SQ   SEQUENCE   535 AA;  57425 MW;  D2D02CD5568AF73C CRC64;
     MTREHQGGTR GLAAARKGFW SGLRFTSLRL RLVLVFGLVA LTAAVSASGI AYWLNREAVL
     TRTQDAVLRD FEQEMQNRAG ALPEHPTQDE VQHTAGQMAN SSQRFSVLLV AENADGTAVY
     GSSGGLGGVA LSDVPESLRT AVNKEQKLTS ANKHPYHLYW QRITDDGTPY LVAGTKVIGG
     GPTGYMLKSL EPEAKDLNSL AWSLGIATAL ALLGSALLAQ ALATTVLKPV HRLGVAARRL
     GEGKLDTRLR VSGTDELADL SRTFNSAAEN LEKRVADMAG REQASRRFVA DMSHELRTPL
     TALTAVTEVL EEELEYAGEG EGEGGSFDPM VEPAVRLVVS ETRRLNDLVE NLMEVTRFDA
     GTARLVLDDV DVADQITACI DARAWLDAVD LDAERGVHAR LDPRRLDVIL ANLIGNALKH
     GGSPVRVSVA RADHEIVIRV RDNGPGIPED VLPHVFDRFY KASASRPRSE GSGLGLSIAL
     ENAHIHGGEI TAENAPEGGA VFTLRLPQDP SPPADEDGGP DEETEDRGKD AKGQV
//