Signal transduction histidine-protein kinase AfsQ2

Preferred order of sections

Names and origin

Protein names

Recommended name:
Signal transduction histidine-protein kinase AfsQ2
EC=2.7.13.3

Gene names

Name:	afsQ2
Ordered Locus Names:	SCO4906
ORF Names:	2SCK8.32c

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism. May activate AfsQ1 by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 535

535

Signal transduction histidine-protein kinase AfsQ2

PRO_0000074683

Regions

Topological domain

1 – 30

30

Cytoplasmic Potential

Transmembrane

31 – 52

22

Helical; Potential

Topological domain

53 – 198

146

Extracellular Potential

Transmembrane

199 – 219

21

Helical; Potential

Topological domain

220 – 535

316

Cytoplasmic Potential

Domain

224 – 276

53

HAMP

Domain

291 – 510

220

Histidine kinase

Amino acid modifications

Modified residue

294

1

Phosphohistidine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q04943 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D2D02CD5568AF73C
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FASTA

535

57,425

        10         20         30         40         50         60 
MTREHQGGTR GLAAARKGFW SGLRFTSLRL RLVLVFGLVA LTAAVSASGI AYWLNREAVL 

        70         80         90        100        110        120 
TRTQDAVLRD FEQEMQNRAG ALPEHPTQDE VQHTAGQMAN SSQRFSVLLV AENADGTAVY 

       130        140        150        160        170        180 
GSSGGLGGVA LSDVPESLRT AVNKEQKLTS ANKHPYHLYW QRITDDGTPY LVAGTKVIGG 

       190        200        210        220        230        240 
GPTGYMLKSL EPEAKDLNSL AWSLGIATAL ALLGSALLAQ ALATTVLKPV HRLGVAARRL 

       250        260        270        280        290        300 
GEGKLDTRLR VSGTDELADL SRTFNSAAEN LEKRVADMAG REQASRRFVA DMSHELRTPL 

       310        320        330        340        350        360 
TALTAVTEVL EEELEYAGEG EGEGGSFDPM VEPAVRLVVS ETRRLNDLVE NLMEVTRFDA 

       370        380        390        400        410        420 
GTARLVLDDV DVADQITACI DARAWLDAVD LDAERGVHAR LDPRRLDVIL ANLIGNALKH 

       430        440        450        460        470        480 
GGSPVRVSVA RADHEIVIRV RDNGPGIPED VLPHVFDRFY KASASRPRSE GSGLGLSIAL 

       490        500        510        520        530 
ENAHIHGGEI TAENAPEGGA VFTLRLPQDP SPPADEDGGP DEETEDRGKD AKGQV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp." Ishizuka H., Horinouchi S., Kieser H.M., Hopwood D.A., Beppu T. J. Bacteriol. 174:7585-7594(1992) [PubMed: 1339426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / NRRL B-16638.
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10654 Genomic DNA. Translation: BAA01503.1. AL939121 Genomic DNA. Translation: CAC33071.1.
PIR	B45270.
RefSeq	NP_629059.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q04943.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1100347.
GenomeReviews	Gene locus SCO4906 in contig AL645882_GR.
KEGG	sco:SCO4906.
NMPDR	fig\|100226.1.peg.4861.
PATRIC	23739650. VBIStrCoe124346_4985.
Phylogenomic databases
HOGENOM	HBG695485.
OMA	LENAHIH.
PhylomeDB	Q04943.
ProtClustDB	CLSK220567.
Enzyme and pathway databases
BRENDA	2.7.13.3. 5998.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
KO	K07654.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFSQ2_STRCO

Accession

Primary (citable) accession number: Q04943

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents