Q04937 (SCRB_LACLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sucrose-6-phosphate hydrolase
Short name=Sucrase
EC=3.2.1.26

Alternative name(s):
Invertase

Gene names

Name:	scrB
Synonyms:	sacA

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.5-7.0.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Sucrose-6-phosphate hydrolase		PRO_0000169873
Regions
	Region	44 – 47	4	Substrate binding By similarity
	Region	106 – 107	2	Substrate binding By similarity
	Region	167 – 168	2	Substrate binding By similarity
Sites
	Active site	47	1	By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	224	1	Substrate By similarity
Experimental info
	Sequence conflict	4	1	S → Q AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q04937 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: D055ADF8321072E5	FASTA	473	54,785
	10 20 30 40 50 60 MKWSTKQRYR TYDSYSESDL ESLRKLALKS PWKSNFHIEP ETGLLNDPNG FSYFNEKWHL 70 80 90 100 110 120 FYQHFPFGPV HGLKSWVHLV SDDLVHFEKT GLVLYPDTKY DNAGVYSGSA LAFENFLFLI 130 140 150 160 170 180 YTGNHRGEDW VRTPYQLGAK IDKNNQLVKF TEPLIYPDFS QTTDHFRDPQ IFSFQGQIYC 190 200 210 220 230 240 LIGAQSSQKN GIIKLYKAIE NNLTDWKDLG NLDFSKEKMG YMIECPNLIF INGRSVLVFC 250 260 270 280 290 300 PQGLDKSIVK YDNIYPNVYV IADDFTTGSK NQLKNAGQLI NLDEGFDCYA TQSFNAPDGS 310 320 330 340 350 360 AYAISWLGLP ETSYPTDKYN VQGVLSMVKK LSIKDNKLYQ YPVEKMKELR QMEQDLLLAD 370 380 390 400 410 420 NNIITSNSYE LEVDFRQQTS TLLSLMTNEK GDSALKVEID KENNTITLIR NYEKRLAHVK 430 440 450 460 470 IEKMNVFIDQ SIFEIFINDG EKVLSDCRVF PNKNQYSIRS QNPIKIKLWE LKK

References

[1]	"Transcriptional regulation of the Tn5276-located Lactococcus lactis sucrose operon and characterization of the sacA gene encoding sucrose-6-phosphate hydrolase." Rauch P.J., de Vos W.M. Gene 121:55-61(1992) [PubMed: 1330831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NIZO R5.
[2]	"Transposon-encoded sucrose metabolism in Lactococcus lactis. Purification of sucrose-6-phosphate hydrolase and genetic linkage to N5-(L-1-carboxyethyl)-L-ornithine synthase in strain K1." Thompson J., Nguyen N.Y., Sackett D.L., Donkersloot J.A. J. Biol. Chem. 266:14573-14579(1991) [PubMed: 1650362] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12, CHARACTERIZATION. Strain: K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96669 Genomic DNA. Translation: AAA25218.1.
PIR	JH0754.
3D structure databases
ProteinModelPortal	Q04937.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. IPR006232. Suc6P_hydrolase. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
Pfam	PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMs	TIGR01322. ScrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_LACLL

Accession

Primary (citable) accession number: Q04937

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	October 19, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program