Q04934 (IVY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein IVY1

Alternative name(s):
Interaction with VPS33 and YPT7 protein 1

Gene names

Name:	IVY1
Ordered Locus Names:	YDR229W
ORF Names:	YD9934.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be required for vacuolar fusion. Overexpression leads to fragmentation of vacuoles, missorting of the vacuolar enzyme carboxypeptidase Y (CPY) to the exterior of the cell and accumulation of multivesicular bodies inside the cell. Ref.3
Subunit structure	Homomultimer. Interacts with YPT7 and VPS33. Ref.3
Subcellular location	Vacuole membrane; Peripheral membrane protein. Note: Binds to various phospholipids. Ref.3
Miscellaneous	Present with 2840 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vacuole fusion, non-autophagic Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	fungal-type vacuole membrane Inferred from direct assay Ref.3. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction Ref.3. Source: IntAct phospholipid binding Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-35255,EBI-35255
VPS33	P20795	3	EBI-35255,EBI-20395
YPT7	P32939	3	EBI-35255,EBI-29509

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Protein IVY1		PRO_0000084277
Regions
	Coiled coil	102 – 122	21	Potential
	Coiled coil	230 – 257	28	Potential
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.8
	Modified residue	14	1	Phosphoserine Ref.8
	Modified residue	15	1	Phosphoserine Ref.8
	Modified residue	59	1	Phosphoserine Ref.6 Ref.8
	Modified residue	84	1	Phosphoserine Ref.7 Ref.8
	Modified residue	85	1	Phosphoserine Ref.5 Ref.7 Ref.8
	Modified residue	88	1	Phosphoserine Ref.8
	Modified residue	90	1	Phosphothreonine Ref.8
	Modified residue	91	1	Phosphoserine Ref.8
	Modified residue	92	1	Phosphothreonine Ref.8
	Modified residue	242	1	Phosphoserine Ref.8
	Modified residue	335	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q04934 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2DB24DD88BED4522	FASTA	453	49,976
	10 20 30 40 50 60 MPDNNTEQLQ GSPSSDQRLR VDWDNGNHFD VSPDRYAPHL SEFYPIVNSK RPVASSAGSE 70 80 90 100 110 120 NNDHLDDMNH LRSSKVYSKA RRASSITSGT STINDLQTLI TKRDVKETQE ALSTLLRNSN 130 140 150 160 170 180 AYSDSLLKTS QNGAEIAHSL ENIAKLKGCN DETAEKLLSA SGLFYLLSNH QLIMSKYFND 190 200 210 220 230 240 LLGDNLIDDI DEFELQTKIM ENKFKAQSKE QSLKLKLQER HNFDISKRKI RNLISYRESL 250 260 270 280 290 300 SSLQARLDQL ETLKHDFYMD SYELVENTCN KVLSKVATVS RAQVEISENI ARKGWSGGGL 310 320 330 340 350 360 DELLCDADDP FSKKADGPYG TIGGDGETAG EAYNSDEETG GNDVVLNELL EGTSQPSTSK 370 380 390 400 410 420 TSLPKSKGSS TVSTPNHSQS SSNKDGVRNN GGGKNGEDED TDNLMGTENS FSLPPTRNSA 430 440 450 EETTQTFKQL SIKEDNDNHS SDTDGMQDQS SNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p." Lazar T., Scheglmann D., Gallwitz D. Eur. J. Cell Biol. 81:635-646(2002) [PubMed: 12553664] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS33 AND YPT7.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND SER-85, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-14; SER-15; SER-59; SER-84; SER-85; SER-88; THR-90; SER-91; THR-92; SER-242 AND SER-335, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48612 Genomic DNA. Translation: CAA88509.1. BK006938 Genomic DNA. Translation: DAA12071.1.
PIR	S59436.
RefSeq	NP_010515.1. NM_001180537.1.
3D structure databases
ProteinModelPortal	Q04934.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2957N.
IntAct	Q04934. 14 interactions.
MINT	MINT-495311.
STRING	Q04934.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR229W; YDR229W; YDR229W.
GeneID	851815.
KEGG	sce:YDR229W.
NMPDR	fig|4932.3.peg.1272.
Organism-specific databases
CYGD	YDR229w.
SGD	S000002637. IVY1.
Phylogenomic databases
eggNOG	fuNOG08498.
GeneTree	EFGT00050000008529.
OMA	HLSEFYP.
OrthoDB	EOG470XSN.
Gene expression databases
ArrayExpress	Q04934.
Genevestigator	Q04934.
GermOnline	YDR229W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	969677.

Entry information

Entry name

IVY1_YEAST

Accession

Primary (citable) accession number: Q04934
Secondary accession number(s): D6VSL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program