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Protein

FACT complex subunit SSRP1

Gene

Ssrp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA. Also acts as a transcriptional coactivator for p63/TP63 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi547 – 61569HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6804756. Regulation of TP53 Activity through Phosphorylation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
FACT complex subunit SSRP1
Alternative name(s):
Facilitates chromatin transcription complex subunit SSRP1
Recombination signal sequence recognition protein 1
Structure-specific recognition protein 1
T160
Gene namesi
Name:Ssrp1
Synonyms:Ciidbp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621143. Ssrp1.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity
  • Nucleusnucleolus By similarity

  • Note: Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 709708FACT complex subunit SSRP1PRO_0000048608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei170 – 1701PhosphothreonineBy similarity
Modified residuei233 – 2331N6-acetyllysineBy similarity
Modified residuei413 – 4131N6-acetyllysineBy similarity
Modified residuei441 – 4411PhosphotyrosineBy similarity
Modified residuei444 – 4441PhosphoserineCombined sources
Modified residuei452 – 4521PhosphotyrosineBy similarity
Modified residuei471 – 4711PhosphoserineBy similarity
Modified residuei510 – 5101Phosphoserine; by CK2By similarity
Modified residuei542 – 5421N6-acetyllysineBy similarity
Modified residuei657 – 6571Phosphoserine; by CK2By similarity
Modified residuei659 – 6591PhosphoserineBy similarity
Modified residuei667 – 6671PhosphoserineBy similarity
Modified residuei668 – 6681PhosphoserineBy similarity
Modified residuei671 – 6711PhosphoserineBy similarity
Modified residuei672 – 6721PhosphoserineBy similarity
Modified residuei673 – 6731PhosphoserineBy similarity
Modified residuei688 – 6881Phosphoserine; by CK2By similarity

Post-translational modificationi

Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity (By similarity).By similarity
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein (By similarity).By similarity
Sumoylated.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei450 – 4512Cleavage; by caspase-3 and/or caspase-7By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ04931.
PRIDEiQ04931.

PTM databases

iPTMnetiQ04931.
PhosphoSiteiQ04931.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008825.
GenevisibleiQ04931. RN.

Interactioni

Subunit structurei

Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Binds to histone H3-H4 tetramers, but not to intact nucleosomes. Interacts with isoform gamma of p63/TP63. Interacts with FYTTD1/UIF and NEK9. Interacts with MYOG (via C-terminal region) (By similarity). Interacts with SRF.By similarity1 Publication

Protein-protein interaction databases

BioGridi249657. 1 interaction.
STRINGi10116.ENSRNOP00000012022.

Structurei

3D structure databases

ProteinModelPortaliQ04931.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi439 – 49658Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi497 – 51115Ser-richAdd
BLAST
Compositional biasi512 – 53423Arg/Lys-rich (basic)Add
BLAST
Compositional biasi623 – 64018Arg/Lys-rich (basic)Add
BLAST
Compositional biasi641 – 70969Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the SSRP1 family.Curated
Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0526. Eukaryota.
COG5165. LUCA.
GeneTreeiENSGT00560000076898.
HOGENOMiHOG000180790.
HOVERGENiHBG002932.
InParanoidiQ04931.
KOiK09272.
OMAiHEVFTTV.
OrthoDBiEOG091G04JP.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR013719. DUF1747.
IPR009071. HMG_box_dom.
IPR011993. PH_dom-like.
IPR000969. SSrcognition.
IPR024954. SSRP1_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF08512. Rtt106. 1 hit.
PF03531. SSrecog. 1 hit.
[Graphical view]
PRINTSiPR00887. SSRCOGNITION.
SMARTiSM00398. HMG. 1 hit.
SM01287. Rtt106. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETLEFNDI FQEVKGSMND GRLRLSRQGI IFKNSKTGKV DNIQAGELTE
60 70 80 90 100
GIWRRVALGH GLKLLTKNGH VYKYDGFRES EFEKLSDFFK THYRLELMEK
110 120 130 140 150
DLCVKGWNWG TVKFGGQLLS FDIGDQPVFE IPLSNVSQCT TGKNEVTLEF
160 170 180 190 200
HQNDDAEVSL MEVRFYVPPT QEDGVDPVEA FAQNVLSKAD VIQATGDAIC
210 220 230 240 250
IFRELQCLTP RGRYDIRIYP TFLHLHGKTF DYKIPYTTVL RLFLLPHKDQ
260 270 280 290 300
RQMFFVISLD PPIKQGQTRY HFLILLFSKD EDISLTLNMN EEEVEKRFEG
310 320 330 340 350
RLTKNMSGSL YEMVSRVMKA LVNRKITVPG NFQGHSGAQC ITCSYKASSG
360 370 380 390 400
LLYPLERGFI YVHKPPVHIR FDEISFVNFA RGTTTTRSFD FEIETKQGTQ
410 420 430 440 450
YTFSSIEREE YGKLFDFVNA KKLNIKNRGL KEGINPGYDD YADSDEDQHD
460 470 480 490 500
AYLERMKEEG KIREENANDS SDDSGEETDE SFNPGEEEED VAEEFDSNAS
510 520 530 540 550
ASSSSNEGDS DREEKKRKQL KRAKMAKDRK SRKKSSEGKK GKDPNAPKRP
560 570 580 590 600
MSAYMLWLNA SREKIKSDHP GISITDLSKK AGEIWKGMSK EKKEEWDRKA
610 620 630 640 650
EDARREYEKA MKEYEGGRGD SSKRDKSKKK KKVKAKLEKK STPSRGSSSK
660 670 680 690 700
SSSRQLSDSF KSKEFVSSDE SSSGENKSKK KRRRSEDSDE EELASTPPSS

EDSASGSDE
Length:709
Mass (Da):80,914
Last modified:December 6, 2005 - v2
Checksum:i3A2FFC192820882F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti382 – 3821G → A in AAA40927 (PubMed:8464746).Curated
Sequence conflicti391 – 3911F → S in AAA40927 (PubMed:8464746).Curated
Sequence conflicti600 – 6001A → G in AAA40927 (PubMed:8464746).Curated
Sequence conflicti609 – 6091K → E in AAA40927 (PubMed:8464746).Curated
Sequence conflicti651 – 6511S → A in AAA40927 (PubMed:8464746).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083588 mRNA. Translation: AAH83588.1.
L08814 mRNA. Translation: AAA40927.1.
PIRiS35637.
RefSeqiNP_112383.1. NM_031121.1.
XP_008760216.1. XM_008761994.1.
UniGeneiRn.35908.

Genome annotation databases

EnsembliENSRNOT00000012022; ENSRNOP00000012022; ENSRNOG00000008825.
ENSRNOT00000091901; ENSRNOP00000073084; ENSRNOG00000008825.
GeneIDi81785.
KEGGirno:81785.
UCSCiRGD:621143. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083588 mRNA. Translation: AAH83588.1.
L08814 mRNA. Translation: AAA40927.1.
PIRiS35637.
RefSeqiNP_112383.1. NM_031121.1.
XP_008760216.1. XM_008761994.1.
UniGeneiRn.35908.

3D structure databases

ProteinModelPortaliQ04931.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249657. 1 interaction.
STRINGi10116.ENSRNOP00000012022.

PTM databases

iPTMnetiQ04931.
PhosphoSiteiQ04931.

Proteomic databases

PaxDbiQ04931.
PRIDEiQ04931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012022; ENSRNOP00000012022; ENSRNOG00000008825.
ENSRNOT00000091901; ENSRNOP00000073084; ENSRNOG00000008825.
GeneIDi81785.
KEGGirno:81785.
UCSCiRGD:621143. rat.

Organism-specific databases

CTDi6749.
RGDi621143. Ssrp1.

Phylogenomic databases

eggNOGiKOG0526. Eukaryota.
COG5165. LUCA.
GeneTreeiENSGT00560000076898.
HOGENOMiHOG000180790.
HOVERGENiHBG002932.
InParanoidiQ04931.
KOiK09272.
OMAiHEVFTTV.
OrthoDBiEOG091G04JP.

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6804756. Regulation of TP53 Activity through Phosphorylation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

PROiQ04931.

Gene expression databases

BgeeiENSRNOG00000008825.
GenevisibleiQ04931. RN.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR013719. DUF1747.
IPR009071. HMG_box_dom.
IPR011993. PH_dom-like.
IPR000969. SSrcognition.
IPR024954. SSRP1_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF08512. Rtt106. 1 hit.
PF03531. SSrecog. 1 hit.
[Graphical view]
PRINTSiPR00887. SSRCOGNITION.
SMARTiSM00398. HMG. 1 hit.
SM01287. Rtt106. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSRP1_RAT
AccessioniPrimary (citable) accession number: Q04931
Secondary accession number(s): Q5XIT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.