Q04929 (CRK_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adapter molecule crk

Alternative name(s):
Proto-oncogene c-Crk
p38

Gene names

Name:

CRK

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May mediate attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. May regulate the EFNA5-EPHA3 signaling By similarity.
Subunit structure	Interacts with ABL1, C3G, SOS, MAP4K1, MAPK8 and DOCK1 via its first SH3 domain. Interacts with BCAR1, CBL, PXN and GAB1 via its SH2 domain upon stimulus-induced tyrosine phosphorylation. Interacts with several tyrosine-phosphorylated growth factor receptors such as EGFR, PDGFR and INSR via its SH2 domain By similarity. Ref.2
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Note: Translocated to the plasma membrane upon cell adhesion By similarity.
Post-translational modification	Phosphorylated on Tyr-222 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-222 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway By similarity. Proline isomerization at Pro-238 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form.
Sequence similarities	Belongs to the CRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Disease	Proto-oncogene
Domain	Repeat SH2 domain SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ephrin receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of Rho GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of actin cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 305

305

Adapter molecule crk

PRO_0000079349

Regions

Domain

13 – 119

107

SH2

Domain

133 – 193

61

SH3 1

Domain

238 – 297

60

SH3 2

Sites

Site

238

1

Proline switch

Amino acid modifications

Modified residue

222

1

Phosphotyrosine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

305

Helix Strand Turn

Details...

Beta strand

137 – 140

4

Beta strand

148 – 151

4

Beta strand

156 – 164

9

Beta strand

166 – 174

9

Beta strand

176 – 178

3

Beta strand

180 – 184

5

Helix

185 – 187

3

Beta strand

200 – 203

4

Beta strand

226 – 228

3

Beta strand

234 – 236

3

Beta strand

239 – 245

7

Beta strand

253 – 255

3

Beta strand

263 – 269

7

Beta strand

272 – 274

3

Beta strand

277 – 280

4

Beta strand

283 – 286

4

Helix

289 – 291

3

Beta strand

292 – 295

4

Sequences

	Sequence		Length	Mass (Da)
	Q04929 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0A79AD232E824768	FASTA	305	33,806
	10 20 30 40 50 60 MAGQFDSEDR GSWYWGRLSR GDAVSLLQGQ RHGTFLVRDS GSIPGDFVLS VSESSRVSHY 70 80 90 100 110 120 IVNSLGPAGG RRAGGEGPGA PGLNPTRFRI GDQEFDSLPS LLEFYKIHYL DTTTLIEPVS 130 140 150 160 170 180 RSRQNSGVIL RQEEVEYVRA LFDFNGNDDE DLPFKKGDIL KIRDKPEEQW WNAEDMDGKR 190 200 210 220 230 240 GMIPVPYVEK CRPSSASVST LTGGNQDSSH PQPLGGPEPG PYAQPSINTP LPNLQNGPFY 250 260 270 280 290 300 ARVIQKRVPN AYDKTALALE VGELVKVTKI NMSGQWEGEC NGKRGHFPFT HVRLLDQQNP DEDFS

References

[1]	"The product of the cellular crk gene consists primarily of SH2 and SH3 regions." Reichman C.T., Mayer B.J., Khawer S., Hanafusa H. Cell Growth Differ. 3:451-460(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural basis for regulation of the Crk signaling protein by a proline switch." Sarkar P., Saleh T., Tzeng S.R., Birge R.B., Kalodimos C.G. Nat. Chem. Biol. 7:51-57(2011) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 135-297, ISOMERIZATION AT PRO-238.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L08168 mRNA. Translation: AAA49001.1.

IPI

IPI00581100.

PIR

A49011.

RefSeq

NP_001007847.1. NM_001007846.1.

UniGene

Gga.27345.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L3P	NMR	-	A	220-297	[»]
2L3Q	NMR	-	A	220-297	[»]
2L3S	NMR	-	A	135-297	[»]

ProteinModelPortal

Q04929.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q04929. 1 interaction.

STRING

9031.ENSGALP00000004174.

Proteomic databases

PaxDb

Q04929.

PRIDE

Q04929.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSGALT00000004183; ENSGALP00000004174; ENSGALG00000002656.

GeneID

417553.

KEGG

gga:417553.

Organism-specific databases

CTD

1398.

Phylogenomic databases

eggNOG

NOG292767.

GeneTree

ENSGT00390000001475.

HOGENOM

HOG000236288.

HOVERGEN

HBG105616.

InParanoid

Q04929.

KO

K04438.

OMA

NQDSSHP.

OrthoDB

EOG4WWRK5.

Family and domain databases

Gene3D

3.30.505.10. 1 hit.

InterPro

IPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]

SUPFAM

SSF50044. SH3. 2 hits.

PROSITE

PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q04929.

NextBio

20820838.

Entry information

Entry name

CRK_CHICK

Accession

Primary (citable) accession number: Q04929

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program