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Protein

Glucosidase 2 subunit beta

Gene

GTB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins. Specifically required for the cleavage of the final glucose.1 Publication

GO - Biological processi

  • N-glycan processing Source: SGD
  • polysaccharide biosynthetic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29801-MONOMER.
ReactomeiR-SCE-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
Alpha-glucosidase 2 subunit beta
Alpha-glucosidase II subunit beta
Glucosidase II subunit beta
Gene namesi
Name:GTB1
Ordered Locus Names:YDR221W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR221W.
SGDiS000002629. GTB1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum lumen Source: SGD
  • glucosidase II complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 702682Glucosidase 2 subunit betaPRO_0000245572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi358 – 3581N-linked (GlcNAc...)Sequence analysis
Glycosylationi520 – 5201N-linked (GlcNAc...)Sequence analysis
Glycosylationi525 – 5251N-linked (GlcNAc...)Sequence analysis
Glycosylationi688 – 6881N-linked (GlcNAc...)Sequence analysis
Glycosylationi699 – 6991N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ04924.

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit alpha (ROT2) and a subunit beta (GTB1).

Binary interactionsi

WithEntry#Exp.IntActNotes
ROT2P381382EBI-37493,EBI-21021

Protein-protein interaction databases

BioGridi32273. 42 interactions.
DIPiDIP-4429N.
IntActiQ04924. 1 interaction.
MINTiMINT-508465.

Structurei

3D structure databases

ProteinModelPortaliQ04924.
SMRiQ04924. Positions 532-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili163 – 22866Sequence analysisAdd
BLAST
Coiled coili478 – 51740Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

GeneTreeiENSGT00510000047770.
HOGENOMiHOG000001046.
InParanoidiQ04924.
OMAiCCDCSDE.
OrthoDBiEOG092C19V4.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR026874. Glucosidase_2_bsu.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 4 hits.
PfamiPF12999. PRKCSH-like. 1 hit.
PF13015. PRKCSH_1. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSMFSLFLL LIEQSPLVAS LQQSQRHIVG VPWEKQHLYD SNEPDLTKWH
60 70 80 90 100
CLNHEDIVLD ISQINDGVCD CPDGSDEPGS AACVEDIFKS VAEGGGKVNK
110 120 130 140 150
YFYCDNKGFI PRYIRKSEVA DGICDCCDCS DELLSGYELF DAGSNCSQLK
160 170 180 190 200
NEFDIMASKE LSSYREGKEA LEELERKYGT KEEAITRGNC LEEDKEKASA
210 220 230 240 250
EIKVLSNRLS ENRAKLEQLR GEYFNQLSHD PILYQFEQLN STRLGSDILT
260 270 280 290 300
SFTMVSRVSK GYQDIFKILS DLSEAYTPSL NDKVVNDNIK KFRKVRRRAE
310 320 330 340 350
KAKINADSKI DDEQADNLYL YFTEEVPQIF LKRESENTLR YVIGKSNFVQ
360 370 380 390 400
ALVEGKINYT NDILEYIREF RLIMDDISQN YNVNFQDAGV KSAVDSYKNY
410 420 430 440 450
LGEYGELAEL EPAHPSESLL RSLSEVTSFV NENAPKVLPP DAVESEQDTN
460 470 480 490 500
SDHIGTSGDL RNKLKEILSK LNIFSSRKDL VSLEKRFRSC ESQVSLLENE
510 520 530 540 550
LKQKMDYKKL LDETEDEGTN STAGNLTELL ELMGSQSYCL DDILDNYVYT
560 570 580 590 600
ICFQRPMTEG VIYQAEDKVD GKKVLIGRFK TSGFNVDLNM EKYAEHLKAT
610 620 630 640 650
YDEKSDLISN LAAIQDDDGN MQHYVFGNLN ELNNGLVLEY ENGDQCWNGP
660 670 680 690 700
RRSATVFVRC SDKFKIRSVH EATKCNYIFD VVGPLGCNKT FEYEPPKFNL

SE
Length:702
Mass (Da):79,907
Last modified:November 1, 1996 - v1
Checksum:i237D9756939D57DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48612 Genomic DNA. Translation: CAA88501.1.
BK006938 Genomic DNA. Translation: DAA12063.1.
PIRiS59428.
RefSeqiNP_010507.3. NM_001180529.3.

Genome annotation databases

EnsemblFungiiYDR221W; YDR221W; YDR221W.
GeneIDi851807.
KEGGisce:YDR221W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48612 Genomic DNA. Translation: CAA88501.1.
BK006938 Genomic DNA. Translation: DAA12063.1.
PIRiS59428.
RefSeqiNP_010507.3. NM_001180529.3.

3D structure databases

ProteinModelPortaliQ04924.
SMRiQ04924. Positions 532-688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32273. 42 interactions.
DIPiDIP-4429N.
IntActiQ04924. 1 interaction.
MINTiMINT-508465.

Proteomic databases

MaxQBiQ04924.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR221W; YDR221W; YDR221W.
GeneIDi851807.
KEGGisce:YDR221W.

Organism-specific databases

EuPathDBiFungiDB:YDR221W.
SGDiS000002629. GTB1.

Phylogenomic databases

GeneTreeiENSGT00510000047770.
HOGENOMiHOG000001046.
InParanoidiQ04924.
OMAiCCDCSDE.
OrthoDBiEOG092C19V4.

Enzyme and pathway databases

BioCyciYEAST:G3O-29801-MONOMER.
ReactomeiR-SCE-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiQ04924.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR026874. Glucosidase_2_bsu.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 4 hits.
PfamiPF12999. PRKCSH-like. 1 hit.
PF13015. PRKCSH_1. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLU2B_YEAST
AccessioniPrimary (citable) accession number: Q04924
Secondary accession number(s): D6VSK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.