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Protein

Glucosidase 2 subunit beta

Gene

GTB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins. Specifically required for the cleavage of the final glucose.1 Publication

Miscellaneous

Present with 9760 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • N-glycan processing Source: SGD
  • polysaccharide biosynthetic process Source: SGD

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29801-MONOMER
YEAST:G3O-29801-MONOMER
ReactomeiR-SCE-901042 Calnexin/calreticulin cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
Alpha-glucosidase 2 subunit beta
Alpha-glucosidase II subunit beta
Glucosidase II subunit beta
Gene namesi
Name:GTB1
Ordered Locus Names:YDR221W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR221W
SGDiS000002629 GTB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000024557221 – 702Glucosidase 2 subunit betaAdd BLAST682

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi358N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi520N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi525N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi688N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi699N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ04924
PaxDbiQ04924
PRIDEiQ04924

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit alpha (ROT2) and a subunit beta (GTB1).

Binary interactionsi

WithEntry#Exp.IntActNotes
ROT2P3813817EBI-37493,EBI-21021

Protein-protein interaction databases

BioGridi32273, 89 interactors
DIPiDIP-4429N
IntActiQ04924, 8 interactors
MINTiQ04924
STRINGi4932.YDR221W

Structurei

3D structure databases

ProteinModelPortaliQ04924
SMRiQ04924
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili163 – 228Sequence analysisAdd BLAST66
Coiled coili478 – 517Sequence analysisAdd BLAST40

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

GeneTreeiENSGT00510000047770
HOGENOMiHOG000001046
InParanoidiQ04924
OMAiVCDCCDC
OrthoDBiEOG092C19V4

Family and domain databases

InterProiView protein in InterPro
IPR026874 Glucosidase_2_bsu
IPR036607 PRKCSH
IPR028146 PRKCSH_N
PANTHERiPTHR12630:SF1 PTHR12630:SF1, 1 hit
PfamiView protein in Pfam
PF12999 PRKCSH-like, 1 hit
PF13015 PRKCSH_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSMFSLFLL LIEQSPLVAS LQQSQRHIVG VPWEKQHLYD SNEPDLTKWH
60 70 80 90 100
CLNHEDIVLD ISQINDGVCD CPDGSDEPGS AACVEDIFKS VAEGGGKVNK
110 120 130 140 150
YFYCDNKGFI PRYIRKSEVA DGICDCCDCS DELLSGYELF DAGSNCSQLK
160 170 180 190 200
NEFDIMASKE LSSYREGKEA LEELERKYGT KEEAITRGNC LEEDKEKASA
210 220 230 240 250
EIKVLSNRLS ENRAKLEQLR GEYFNQLSHD PILYQFEQLN STRLGSDILT
260 270 280 290 300
SFTMVSRVSK GYQDIFKILS DLSEAYTPSL NDKVVNDNIK KFRKVRRRAE
310 320 330 340 350
KAKINADSKI DDEQADNLYL YFTEEVPQIF LKRESENTLR YVIGKSNFVQ
360 370 380 390 400
ALVEGKINYT NDILEYIREF RLIMDDISQN YNVNFQDAGV KSAVDSYKNY
410 420 430 440 450
LGEYGELAEL EPAHPSESLL RSLSEVTSFV NENAPKVLPP DAVESEQDTN
460 470 480 490 500
SDHIGTSGDL RNKLKEILSK LNIFSSRKDL VSLEKRFRSC ESQVSLLENE
510 520 530 540 550
LKQKMDYKKL LDETEDEGTN STAGNLTELL ELMGSQSYCL DDILDNYVYT
560 570 580 590 600
ICFQRPMTEG VIYQAEDKVD GKKVLIGRFK TSGFNVDLNM EKYAEHLKAT
610 620 630 640 650
YDEKSDLISN LAAIQDDDGN MQHYVFGNLN ELNNGLVLEY ENGDQCWNGP
660 670 680 690 700
RRSATVFVRC SDKFKIRSVH EATKCNYIFD VVGPLGCNKT FEYEPPKFNL

SE
Length:702
Mass (Da):79,907
Last modified:November 1, 1996 - v1
Checksum:i237D9756939D57DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48612 Genomic DNA Translation: CAA88501.1
BK006938 Genomic DNA Translation: DAA12063.1
PIRiS59428
RefSeqiNP_010507.3, NM_001180529.3

Genome annotation databases

EnsemblFungiiYDR221W; YDR221W; YDR221W
GeneIDi851807
KEGGisce:YDR221W

Similar proteinsi

Entry informationi

Entry nameiGLU2B_YEAST
AccessioniPrimary (citable) accession number: Q04924
Secondary accession number(s): D6VSK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health