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Q04916

- VP4_ROTGA

UniProt

Q04916 - VP4_ROTGA

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Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.
VP8* forms the head of the spikes By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei206 – 2072Cleavage Reviewed prediction
Sitei213 – 2142Cleavage Reviewed prediction

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifieri10942 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.
Chain Outer capsid protein VP8* : Virion By similarity
Note: Outer capsid protein By similarity.
Chain Outer capsid protein VP5* : Virion By similarity
Note: Outer capsid protein By similarity.

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Outer capsid protein VP4PRO_0000149538Add
BLAST
Chaini1 – 206206Outer capsid protein VP8* Reviewed predictionPRO_0000369838Add
BLAST
Chaini214 – 749536Outer capsid protein VP5* Reviewed predictionPRO_0000369839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi53 – 531N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi109 – 1091N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi133 – 1331N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi407 – 4071N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi527 – 5271N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi568 – 5681N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi620 – 6201N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi681 – 6811N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi698 – 6981N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer Reviewed prediction.

Structurei

3D structure databases

ProteinModelPortaliQ04916.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP4 family.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04916-1 [UniParc]FASTAAdd to Basket

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MLTYLRREWQ SFGETVTIKN TFNAQEDNNQ SGRKTDNRPV KTEGRYCYKA    50
DVNRSKYYHD VQGFSLGQSD LHIDPTQFIM YSGTISNGIS YVNQAPSCVQ 100
LSLKFTPGNS SLIEDLHIEP YKVEVLKIEH VGNVSRATLL SDIVSLSIAQ 150
KKLLLYGFTQ LGIQGLTGDV VSVETKRIPT PTQTNLLTIE DSMQCFTWDM 200
NCANVRSTKQ DSRLIIYEQE DGFWKIVTET LSIKVKPYFK AYGTMGGAFK 250
NWLVDSGFEK YQHDLAYVRD GVTVNAHTIT YVNPSGKAGL QQDWRPATDY 300
NGQITVLQPG DGFSVWYYED KWQINQAIYA KNFQSDTRAQ GYLENVGTLK 350
FKMNYIPAFA EIRNKPGKVN YAYLNGGFAQ VDASGYTGMS IILNFVCTGE 400
RFYASDNNSR VDNKITPFIS YIGDYYTLSG GDFYRQGCCA GFAAGYDDVS 450
PEHGITVSYT VMKPSDPDFI TGGENYGESI TSDLEVSIRN LQDQINSIIA 500
EMNIQQVTSA VFTAITNLGE LPGLFSNITK VFSKTKEALS KLKSRKKTSP 550
MPIAATSIID KTTVDVPNLT IVNKMPEEYE LGIIYNSMRT KKLIEQKKHD 600
FSTFTVATEV KLPYISKATN FSDQFMTSIS SRGITIGKSD IIQYDPMNNI 650
LSAMNRKNAQ IINYKIDPDL AHEVLSQMST NATRSLFSLN VRKQLHINNS 700
FDTPTYGQLV ERILDDGQLL DILGKLNPNS VEELFSEFLH RIQHQLREY 749
Length:749
Mass (Da):84,362
Last modified:June 1, 1994 - v1
Checksum:iD1223527DEAE0F21
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91434 mRNA. Translation: AAA47338.1.
PIRiA45687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91434 mRNA. Translation: AAA47338.1 .
PIRi A45687.

3D structure databases

ProteinModelPortali Q04916.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV."
    Mackow E.R., Werner-Eckert R., Fay M.E., Tao H., Chen G.-M.
    J. Virol. 67:2730-2738(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP4_ROTGA
AccessioniPrimary (citable) accession number: Q04916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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