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Q04916 (VP4_ROTGA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifier10942 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length749 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion By similarity. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 749749Outer capsid protein VP4
PRO_0000149538
Chain1 – 206206Outer capsid protein VP8* Potential
PRO_0000369838
Chain214 – 749536Outer capsid protein VP5* Potential
PRO_0000369839

Sites

Site206 – 2072Cleavage Potential
Site213 – 2142Cleavage Potential

Amino acid modifications

Glycosylation291N-linked (GlcNAc...); by host Potential
Glycosylation531N-linked (GlcNAc...); by host Potential
Glycosylation1091N-linked (GlcNAc...); by host Potential
Glycosylation1331N-linked (GlcNAc...); by host Potential
Glycosylation4071N-linked (GlcNAc...); by host Potential
Glycosylation5271N-linked (GlcNAc...); by host Potential
Glycosylation5681N-linked (GlcNAc...); by host Potential
Glycosylation6201N-linked (GlcNAc...); by host Potential
Glycosylation6811N-linked (GlcNAc...); by host Potential
Glycosylation6981N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q04916 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D1223527DEAE0F21

FASTA74984,362
        10         20         30         40         50         60 
MLTYLRREWQ SFGETVTIKN TFNAQEDNNQ SGRKTDNRPV KTEGRYCYKA DVNRSKYYHD 

        70         80         90        100        110        120 
VQGFSLGQSD LHIDPTQFIM YSGTISNGIS YVNQAPSCVQ LSLKFTPGNS SLIEDLHIEP 

       130        140        150        160        170        180 
YKVEVLKIEH VGNVSRATLL SDIVSLSIAQ KKLLLYGFTQ LGIQGLTGDV VSVETKRIPT 

       190        200        210        220        230        240 
PTQTNLLTIE DSMQCFTWDM NCANVRSTKQ DSRLIIYEQE DGFWKIVTET LSIKVKPYFK 

       250        260        270        280        290        300 
AYGTMGGAFK NWLVDSGFEK YQHDLAYVRD GVTVNAHTIT YVNPSGKAGL QQDWRPATDY 

       310        320        330        340        350        360 
NGQITVLQPG DGFSVWYYED KWQINQAIYA KNFQSDTRAQ GYLENVGTLK FKMNYIPAFA 

       370        380        390        400        410        420 
EIRNKPGKVN YAYLNGGFAQ VDASGYTGMS IILNFVCTGE RFYASDNNSR VDNKITPFIS 

       430        440        450        460        470        480 
YIGDYYTLSG GDFYRQGCCA GFAAGYDDVS PEHGITVSYT VMKPSDPDFI TGGENYGESI 

       490        500        510        520        530        540 
TSDLEVSIRN LQDQINSIIA EMNIQQVTSA VFTAITNLGE LPGLFSNITK VFSKTKEALS 

       550        560        570        580        590        600 
KLKSRKKTSP MPIAATSIID KTTVDVPNLT IVNKMPEEYE LGIIYNSMRT KKLIEQKKHD 

       610        620        630        640        650        660 
FSTFTVATEV KLPYISKATN FSDQFMTSIS SRGITIGKSD IIQYDPMNNI LSAMNRKNAQ 

       670        680        690        700        710        720 
IINYKIDPDL AHEVLSQMST NATRSLFSLN VRKQLHINNS FDTPTYGQLV ERILDDGQLL 

       730        740 
DILGKLNPNS VEELFSEFLH RIQHQLREY 

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References

[1]"Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV."
Mackow E.R., Werner-Eckert R., Fay M.E., Tao H., Chen G.-M.
J. Virol. 67:2730-2738(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91434 mRNA. Translation: AAA47338.1.
PIRA45687.

3D structure databases

ProteinModelPortalQ04916.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameVP4_ROTGA
AccessionPrimary (citable) accession number: Q04916
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families