Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q04916

- VP4_ROTGA

UniProt

Q04916 - VP4_ROTGA

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.By similarity
    VP8* forms the head of the spikes.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei206 – 2072CleavageSequence Analysis
    Sitei213 – 2142CleavageSequence Analysis

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
    Taxonomic identifieri10942 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion By similarity
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion By similarity
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 749749Outer capsid protein VP4PRO_0000149538Add
    BLAST
    Chaini1 – 206206Outer capsid protein VP8*Sequence AnalysisPRO_0000369838Add
    BLAST
    Chaini214 – 749536Outer capsid protein VP5*Sequence AnalysisPRO_0000369839Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi53 – 531N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi109 – 1091N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi133 – 1331N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi407 – 4071N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi527 – 5271N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi568 – 5681N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi620 – 6201N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi681 – 6811N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi698 – 6981N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ04916.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04916-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTYLRREWQ SFGETVTIKN TFNAQEDNNQ SGRKTDNRPV KTEGRYCYKA    50
    DVNRSKYYHD VQGFSLGQSD LHIDPTQFIM YSGTISNGIS YVNQAPSCVQ 100
    LSLKFTPGNS SLIEDLHIEP YKVEVLKIEH VGNVSRATLL SDIVSLSIAQ 150
    KKLLLYGFTQ LGIQGLTGDV VSVETKRIPT PTQTNLLTIE DSMQCFTWDM 200
    NCANVRSTKQ DSRLIIYEQE DGFWKIVTET LSIKVKPYFK AYGTMGGAFK 250
    NWLVDSGFEK YQHDLAYVRD GVTVNAHTIT YVNPSGKAGL QQDWRPATDY 300
    NGQITVLQPG DGFSVWYYED KWQINQAIYA KNFQSDTRAQ GYLENVGTLK 350
    FKMNYIPAFA EIRNKPGKVN YAYLNGGFAQ VDASGYTGMS IILNFVCTGE 400
    RFYASDNNSR VDNKITPFIS YIGDYYTLSG GDFYRQGCCA GFAAGYDDVS 450
    PEHGITVSYT VMKPSDPDFI TGGENYGESI TSDLEVSIRN LQDQINSIIA 500
    EMNIQQVTSA VFTAITNLGE LPGLFSNITK VFSKTKEALS KLKSRKKTSP 550
    MPIAATSIID KTTVDVPNLT IVNKMPEEYE LGIIYNSMRT KKLIEQKKHD 600
    FSTFTVATEV KLPYISKATN FSDQFMTSIS SRGITIGKSD IIQYDPMNNI 650
    LSAMNRKNAQ IINYKIDPDL AHEVLSQMST NATRSLFSLN VRKQLHINNS 700
    FDTPTYGQLV ERILDDGQLL DILGKLNPNS VEELFSEFLH RIQHQLREY 749
    Length:749
    Mass (Da):84,362
    Last modified:June 1, 1994 - v1
    Checksum:iD1223527DEAE0F21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91434 mRNA. Translation: AAA47338.1.
    PIRiA45687.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91434 mRNA. Translation: AAA47338.1 .
    PIRi A45687.

    3D structure databases

    ProteinModelPortali Q04916.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV."
      Mackow E.R., Werner-Eckert R., Fay M.E., Tao H., Chen G.-M.
      J. Virol. 67:2730-2738(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiVP4_ROTGA
    AccessioniPrimary (citable) accession number: Q04916
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3